Abstract:
:This study aims to investigate the apoptotic and anti-angiogenic effect of Zoledronic acid on hormone-refractory prostate cancer cell lines. XTT cell proliferation assay used to assess cytotoxicity. Caspase 3/7 activity and DNA fragmentation were measured to verify apoptosis. Angiogenic cytokine profiles investigated using the human angiogenesis antibody array I. Administration of Zoledronic acid on PC-3 and DU-145 prostate cancer cell lines resulted in increased cytotoxicity and apoptosis with a time- and dose-related manner. Also, the administration of Zoledronic acid significantly reduced several angiogenic cytokine productions in PC-3 and DU-145 cell lines. Zoledronic acid successfully induced apoptosis and reduced various angiogenic cytokine production in hormone-refractory prostate cancer cell lines. Novel treatment protocols may be developed in the future with chemotherapeutic combinations for the treatment of prostate cancer.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Gokalp Cdoi
10.1007/s11033-020-05840-6subject
Has Abstractpub_date
2020-10-01 00:00:00pages
7675-7683issue
10eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-05840-6journal_volume
47pub_type
杂志文章abstract::The full length cDNA sequence of the Tpt1/TCTP (Tumor protein, Translationally-controlled1) gene was identified from Common Carp (Cyprinus carpio, Cyprinidae), and was designated as CcTpt1 gene. The CDS is 510 bp and encodes a 170-amino acid peptide with a typical Tpt1 signature 2 domain, and is a typical Tpt1 protein...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2806-x
更新日期:2013-10-13 00:00:00
abstract::To gain genetic insights into the protein-rich microalga, the transcriptome of Arthrospira platensis was sequenced using Illumina technology and de novo assembly was carried out. A total of 6023 transcripts were present in the transcriptome among which 4616 transcripts were annotated with specific functions. Gene onto...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4229-1
更新日期:2018-10-01 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) in adiponectin gene [rs1501299 (+276G/T) and rs266729 (-11377C/G)] and one SNP of leptin gene [rs7799039 (-2548G/A)] are known to influence plasma levels of adiponectin and leptin respectively. Literature is scarce on the association of adiponectin gene polymorphism rs266729 with...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05070-5
更新日期:2019-12-01 00:00:00
abstract::Osteoporosis is a common health problem in Mexico, so it is essential to investigate the status of different gene polymorphisms that could serve as genetic susceptibility markers in the Mexican population. Genes with a role in bone metabolism are excellent candidates for association studies. In this study were determi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2357-6
更新日期:2013-03-01 00:00:00
abstract::Isolated, deproteinized nucleic acids from calf thymus and rat liver nuclei were fractionated on Bio-Gel A-5m into DNA and RNA fractions. Extraction of deproteinized DNA and nuclear RNA with 80% ethanol at pH 9.5 yielded low molecular weight peptides (deprimerones) capable of inhibiting DNA transcription in a cell-fre...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00777243
更新日期:1982-04-16 00:00:00
abstract::The present study was designed to investigate the protective effect of ferulic acid (FA) against isoproterenol (ISO)-induced cardiac toxicity in rats. Isoproterenol challenged in a dose of 85 mg/kg body weight (b.w.) subcutaneously for two consecutive days in the experimental group resulted in acute cardiac toxicity a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4297-2
更新日期:2018-10-01 00:00:00
abstract::Gibberellic acid (GA) is involved in the regulation of plant growth and development. We defined GA-stimulated transcript (GAST) gene family and characterized its four members (TaGAST1, 2, 3, and 4) in wheat spikes. Triticum aestivum whole spikes were collected at ten developmental stages and dehulled spikelets were ob...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-4077-9
更新日期:2016-12-01 00:00:00
abstract::Dengue virus and Zika virus are arthropod-borne flaviviruses that cause millions of infections worldwide. The co-circulation of both viruses makes serological diagnosis difficult as they share high amino acid similarities in viral proteins. Antigens are one of the key reagents in the differential diagnosis of these vi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05772-1
更新日期:2020-10-01 00:00:00
abstract::A nucleic acid sequence MC, encoding Momordica Chanrantia anti-hyperglycaemic peptide MC6 (accession: AAX06814) synthesized according to Escherichia coli preferred codons, was cloned and expressed in E. coli. Recombinant protein pQE8-MC (about 3.5 kDa) was purified and analyzed by 20% SDS-PAGE and western blot. It rev...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9609-0
更新日期:2010-04-01 00:00:00
abstract::Lac insects are commercial scale insects with high economic value. The combined molecular phylogeny of 20 lac insect populations was generated using elongation factor 1 alpha, mitochondrial cytochrome c oxidase subunit I and the small subunit ribosomal RNA gene loci. The 20 populations of lac insects clustered into fo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2701-5
更新日期:2013-10-01 00:00:00
abstract::The aim of this study was to elucidate the effects of sex hormones on activity of the ABCG2 promoter in different cell lines. T47D cells and BeWo cells were used as models for ABCG2-expressing cell lines, and luciferase assays using ABCG2 promoter-luciferase constructs were performed. It was shown that progesterone in...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9395-0
更新日期:2009-09-01 00:00:00
abstract::Nuclear magnetic resonance (NMR) spectroscopy is a useful biophysical technique to study the ligand-protein interaction. In this report, we have used bacterially produced ERβ and its domains for studying the functional analysis of ligand-protein interaction. Briefly, ERβ and its transactivation domain (TAD) and ligand...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0600-6
更新日期:2011-10-01 00:00:00
abstract::Molluscs are an extraordinarily diverse group of animals and to discriminate them based on one molecular marker/gene is very difficult because of the too fast or slow rate of nucleotide substitution. In the study, the tropomyosin cds (coding sequences) of 43 animal species were analyzed, the results of which suggested...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0591-3
更新日期:2011-10-01 00:00:00
abstract::Our previous study revealed that CRB3 protein expression was reduced in clear cell renal cell carcinoma (ccRCC) and was associated with TNM stage, pathological grade, and poor prognosis of ccRCC. This study aimed to investigate if DNA methylation of CRB3 decreases its expression, subsequently leading to the progressio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04892-7
更新日期:2019-08-01 00:00:00
abstract::The rapid progress made over the last 10 years in the identification of individual autoantigens and in the localization of the epitopes involved, has resulted in a parallel reduction in the complexity of the antigen required for the detection of autoantibodies. The ability to use synthetic peptides as antigens is a re...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00464709
更新日期:1992-06-01 00:00:00
abstract::Methylene tetrahydrofolate reductase (MTHFR) C677T polymorphism shows considerable heterogeneity in its distribution in humans worldwide. The current study was conducted to investigate whether this polymorphism exhibited adaptive developmental plasticity in the control of the TT-genotype frequency. We screened 1,818 S...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3163-0
更新日期:2014-05-01 00:00:00
abstract::Sheep are valuable resources for the wool industry. Wool growth of Aohan fine wool sheep has cycled during different seasons in 1 year. Therefore, identifying genes that control wool growth cycling might lead to ways for improving the quality and yield of fine wool. In this study, we employed Agilent sheep gene expres...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3405-1
更新日期:2014-08-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that function in transcriptional and post-transcriptional regulation of gene expression. Several miRNAs have been implicated in regulating prostate cancer (PCa) progression. Deregulations of miRNA regulatory networks have been reported in ERG positive PCa, which accounts fo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-016-3948-4
更新日期:2016-04-01 00:00:00
abstract::Selenium compounds have been widely investigated as novel anticancer agents due to high efficacy and selectivity against cancer cells in recent years. This study aimed to research the potential inhibitory effects of seleno-β-lactoglobulin (Se-β-Lg) on HepG2 cells in vitro. MTT results demonstrated that the synthetized...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04953-x
更新日期:2019-10-01 00:00:00
abstract::Wnts are secreted lipid-modified signaling proteins. Activation of Wnt signalling in many tissues has also been associated with cancer. In many eukaryotes, expression of nuclear-encoded mRNA can be strongly inhibited by the presence of a small double-stranded RNA corresponding to exon sequences in the mRNA. In this st...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9055-9
更新日期:2008-06-01 00:00:00
abstract::Previous data on association between CYP2E1 Rsa I/Pst I polymorphism and oral cancer risk were controversial. To investigate the association between CYP2E1 Rsa I/Pst I polymorphism and oral cancer risk. We performed a meta-analysis to assess the relationship between oral cancer and genotype with English language until...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-011-0777-3
更新日期:2012-01-01 00:00:00
abstract::Osteoporosis is a degenerative disease of the skeletal system, and its major complication is fracture that severely influences the living quality of the middle-aged and the aged. The purpose of this study was to investigate the significance of sex hormones and some biochemical indicators related to bone metabolism in ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0187-y
更新日期:2011-02-01 00:00:00
abstract::Microsatellites, or simple sequence repeats (SSRs), are very useful molecular markers for a number of plant species. We used a new publicly available module (TROLL) to extract microsatellites from the public database of soybean expressed sequence tag (EST) sequences. A total of 12,833 sequences containing di- to penta...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1776-8
更新日期:2012-09-01 00:00:00
abstract::Echinococcus granulosus is a parasitic helminth which affects both man and animals. During infection with larval stage of the organism secretory and membrane-bound (S/M) proteins play a meaningful role for evasion of immune system. Antigen B (AgB) is one of them. Present investigation has defined sequence diversity of...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0332-7
更新日期:2011-03-01 00:00:00
abstract::Mouse U26 has been defined as a 2-aminoadipic 6-semialdehyde dehydrogenase. It was speculated to be a PQQ-dependent AAS dehydrogenase due to the research of demonstrating PQQ as a new B vitamin. We isolated a novel human cDNA from the human fetal brain cDNA library we constructed. Its deduced protein was most related ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-003-2716-4
更新日期:2005-03-01 00:00:00
abstract::Ebselen, an organoselenium compound, mimics glutathione peroxidase activity. It is a multifunctional compound, which catalyzes several essential reactions for the protection of cellular components from oxidative and free radical damage. Based on a number of in vitro and in vivo studies, various mechanisms are proposed...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3417-x
更新日期:2014-08-01 00:00:00
abstract::Apolipoprotein AV (apoAV) modulates plasma triglyceride levels, which is an independent risk factor for cardiovascular disease. ApoAV is also involved in atherosclerosis lesion formation. In order to systematically evaluate the apolipoprotein-related gene profile in tree shrew, a model for its insusceptibility to athe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2641-0
更新日期:2013-09-01 00:00:00
abstract::An RNA polymerase III transcript, 7SK nuclear RNA, was found to bear sequence homology to the B2 class of highly repeated elements in the mouse genome. Northern blot hybridizations between the small RNAs and two B2 clones showed that only one of them (p49C8) hybridized to 7SK RNA. Both clones, however, hybridized to 4...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00580650
更新日期:1987-01-01 00:00:00
abstract::Tumor microenvironment (TME) crucially functions in tumor initiation and progression. Stroma-tumor interactions and cellular transdifferentiation are the prerequisite for tumor formation. Transforming growth factor-β (TGF-β), a major cytokine secreted by tumor-associated fibroblasts (TAFs) and cancer cells, is a cruci...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04719-5
更新日期:2019-06-01 00:00:00
abstract::Hereditary hearing loss is a genetically heterogeneous disorder. Mutations in connexin 26 (CX26), are a major cause in many countries and are largely dependent on ethnic groups. The purpose of our study was to evaluate the prevalence of GJB2 mutations among affected individuals from south of Iran. Fifty patients prese...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1929-9
更新日期:2012-12-01 00:00:00