A strategy based on Amplified Fragment Length Polymorphism (AFLP) for routine genotyping of nontuberculous mycobacteria at the clinical laboratory.

Abstract:

:The increasing worldwide incidence of mycobacteriosis and the need to achieve improved clinical management makes nontuberculous mycobacteria (NTM) genotyping a useful tool. However, because of technical difficulties, medium size microbiology laboratories do not attempt to compare the genetic patterns that each of their isolates present. We have aimed to optimize a genotyping method with a reduced hands-on experimental time and that requires few technical resources. A strategy based on the Amplified Fragment Length Polymorphism (AFLP) methodology was developed using two rare-cutters enzymes (SacI and BglII). One out of seven primers was sequentially used in each amplification reaction that was analyzed by agarose gel electrophoresis. This approach makes it possible the timely genotyping of a moderate number of strains and its characterization without the need of image analysis software. We have genotyped 28 Mycobacterium intracellulare and 4 M. abscessus. Clinical researchers are encouraged to routinely genotype their NTM isolates.

journal_name

Mol Biol Rep

authors

Blanco-Conde S,González-Cortés C,López-Medrano R,Palacios-Gutiérrez JJ,Diez-Tascón C,Nebreda-Mayoral T,Sierra-García MJ,Rivero-Lezcano OM

doi

10.1007/s11033-020-05420-8

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

3397-3405

issue

5

eissn

0301-4851

issn

1573-4978

pii

10.1007/s11033-020-05420-8

journal_volume

47

pub_type

杂志文章
  • Association between the CTLA-4 +49 A/G polymorphism and susceptibility to rheumatoid arthritis: a meta-analysis.

    abstract::The aim of this study was to explore whether the cytotoxic T lymphocyte antigen-4 (CTLA-4) +49 A/G polymorphism confers susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between CTLA-4 +49 A/G polymorphism and RA using; 1) allele contrast, 2) the recessive model, 3) the dom...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-011-1364-3

    authors: Lee YH,Bae SC,Choi SJ,Ji JD,Song GG

    更新日期:2012-05-01 00:00:00

  • In vitrocleavage of 45S pre-ribosomal RNA and of giant heterogeneous RNA extracted from human leukemic cells.

    abstract::45S ribosomal precursor RNA and large heterogeneous RNA molecules (greater than 45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments sedimenting at 28S a...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00808381

    authors: Torelli UL,Ferrari S,Torelli GM,Cadossi R,Ferrari S,Montagnani G,Narni F

    更新日期:1977-12-01 00:00:00

  • Vector-free in vivo trans-determination of adult hepatic stem cells to insulin-producing cells.

    abstract::A reduction in the number of functional β-cells is the central pathological event in diabetes. Liver and ventral pancreas differentiates simultaneously in the same general domain of cells within embryonic endoderm. In addition, the precursor cell population being bi-potential may be targeted for either pancreas or liv...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04870-z

    authors: Sarkar S,Munshi C,Chatterjee S,Mukherjee S,Bhattacharya S

    更新日期:2019-10-01 00:00:00

  • Familial Mediterranean fever in Syrian patients: MEFV gene mutations and genotype-phenotype correlation.

    abstract::Familial Mediterranean fever is an autosomal recessive disorder characterized by recurrent attacks of abdominal pain, synovitis and pleuritis. MEFV gene mutations are responsible for the disease. The objective of this study was to identify the frequency and distribution of 12 MEFV mutations in 153 Syrian patients and ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-009-9475-9

    authors: Jarjour RA

    更新日期:2010-01-01 00:00:00

  • Computational analysis of human N-acetylgalactosamine-6-sulfate sulfatase enzyme: an update in genotype-phenotype correlation for Morquio A.

    abstract::Mucopolysaccharidosis IV A (MPS IV A) is a lysosomal storage disease produced by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme. Although genotype-phenotype correlations have been reported, these approaches have not enabled to establish a complete genotype-phenotype correlation, and they ha...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3383-3

    authors: Olarte-Avellaneda S,Rodríguez-López A,Alméciga-Díaz CJ,Barrera LA

    更新日期:2014-11-01 00:00:00

  • Identification, characterization, and expression analysis of a serotonin receptor involved in the reproductive process of the Pacific abalone, Haliotis discus hannai.

    abstract::Serotonin receptor (5-HT) is a biogenic amine acting as a neurotransmitter and neuromodulator that mediates various aspects of reproduction and gametogenesis. The full-length nucleotide sequence of Haliotis discus hannai encodes a protein of 417 amino acids with a predicted molecular mass of 46.54 kDa and isoelectric ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05162-2

    authors: Sharker MR,Sukhan ZP,Kim SC,Lee WK,Kho KH

    更新日期:2020-01-01 00:00:00

  • New uracil analogs as downregulators of ABC transporters in 5-fluorouracil-resistant human leukemia HL-60 cell line.

    abstract::Overexpression of ATP-binding cassette (ABC) transporters causing multidrug resistance (MDR) in cancer cells is one of the major obstacles in cancer chemotherapy. The 5-FU resistant subclone (HL-60/5FU) of the human HL-60 promyelocytic leukemia cell line was selected by the conventional method of continuous exposure o...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05017-w

    authors: Długosz-Pokorska A,Pięta M,Janecki T,Janecka A

    更新日期:2019-12-01 00:00:00

  • Isolation and characterization of a cotton cdh-like gene.

    abstract::Cotton fiber cells elongate without dividing to form economically valuable spinnable fiber. Reports of the ploidy level of fiber cells are variable. Early reports indicated an increase in nuclear DNA content in young fibers; however, subsequent reports failed to observe such a significant increase in ploidy level. Eva...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-009-9496-4

    authors: Taliercio E,Ray J,Scheffler J

    更新日期:2010-02-01 00:00:00

  • Quantitative expression of microRNAs in Brassica oleracea infected with Xanthomonas campestris pv. campestris.

    abstract::Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). MicroRNAs (miRNAs) play an important role in plant defense modulation and therefore the analysis of these molecules can help better understand plant-pathogen ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04779-7

    authors: Santos LS,Maximiano MR,Megias E,Pappas M,Ribeiro SG,Mehta A

    更新日期:2019-06-01 00:00:00

  • Cytokine gene polymorphism and graft-versus-host disease: a survey in Iranian bone marrow transplanted patients.

    abstract::Graft versus host disease (GVHD) is a major complication of bone marrow transplantation (BMT). Numerous studies have shown the potential role of cytokine genotypes in the occurrence of GVHD. In this retrospective, case-control study we aimed to investigate the association between 13 cytokine genes and acute GVHD (aGVH...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2585-4

    authors: Noori-Daloii MR,Jalilian N,Izadi P,Sobhani M,Rabii-Gilani Z,Yekaninejad MS

    更新日期:2013-08-01 00:00:00

  • Identification of two AFLP markers linked to bacterial wilt resistance in tomato and conversion to SCAR markers.

    abstract::Tomato bacterial wilt (BW) incited by Ralstonia solanacearum is a constraint on tomato production in tropical, subtropical and humid regions of the world. In this paper, we present the results of a research aimed at the identification of PCR-based markers amplified fragment length polymorphism (AFLP) linked to the gen...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-007-9204-1

    authors: Miao L,Shou S,Cai J,Jiang F,Zhu Z,Li H

    更新日期:2009-03-01 00:00:00

  • Functional characterization of a Δ5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.

    abstract::Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3633-4

    authors: Liu H,Guo Z,Zheng H,Wang S,Wang Y,Liu W,Zhang G

    更新日期:2014-11-01 00:00:00

  • Perspectives on zinc finger protein function and evolution--an update.

    abstract::Complexity is one of the hallmarks that applies to C2H2 type zinc finger proteins (ZFPs). Structurally distinct clusters of zinc finger modules define an extremely large superfamily of nucleic acid binding proteins with several hundred, perhaps thousands of different members in vertebrates. Recent discoveries have pro...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1007/BF00999848

    authors: Pieler T,Bellefroid E

    更新日期:1994-07-01 00:00:00

  • Plasma lipids and lipoproteins in children and young adults with major β-thalassemia from western Iran: influence of genotype.

    abstract::To determine the plasma lipid and lipoprotein profiles and their possible association with the type of β-thalassemia mutation we studied 103 major β-thalassemia patients including 71 children and 32 young adults compared to 102 healthy subjects consisted of 90 children and 12 young healthy adults. The plasma lipid and...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0397-3

    authors: Madani H,Rahimi Z,Manavi-Shad M,Mozafari H,Akramipour R,Vaisi-Raygani A,Rezaei M,Malek-Khosravi S,Shakiba E,Parsian A

    更新日期:2011-04-01 00:00:00

  • Phenylalanyl-tRNA synthetase from E. coli MRE-600: localization of the phenylalanine binding sites on the subunits by affinity reagents.

    abstract::The phenylalanine and the phenylalanyl-tRNAPhe binding sites on the subunits of phenylalanyl-tRNA synthetase from E. coli MRE-600 were localized using p-azidoanilidate of [14C]phenylalanine and N-bromoacetyl-[14C]phenylalanyl-tRNAPhe. The phenylalanine recognizing site was shown to be situated on the alpha subunit of ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00778515

    authors: Lavrik OI,Moor NA,Khodyreva SN

    更新日期:1982-03-31 00:00:00

  • Vascular endothelial growth factor (VEGF) gene polymorphisms and risk of head and neck cancer: a meta-analysis involving 2,444 individuals.

    abstract::The association between vascular endothelial growth factor (VEGF) gene polymorphisms and risk of head and neck cancer (HNC) were investigated in many published studies; however, the currently available results are inconclusive. Therefore, we conducted this meta-analysis for deriving a more precise estimation of associ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-013-2708-y

    authors: Leng WD,He MN,Chen QL,Gong H,Zhang L,Zeng XT

    更新日期:2013-10-01 00:00:00

  • Genome-wide pathway analysis of genome-wide association studies on systemic lupus erythematosus and rheumatoid arthritis.

    abstract::The aim of this study was to explore candidate single nucleotide polymorphisms (SNPs) and candidate mechanisms of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Two SLE genome-wide association studies (GWASs) datasets were included in this study. Meta-analysis was conducted using 737,984 SNPs in 1,5...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-012-1952-x

    authors: Lee YH,Bae SC,Choi SJ,Ji JD,Song GG

    更新日期:2012-12-01 00:00:00

  • Association of genetic variants for FABP3 gene with back fat thickness and intramuscular fat content in pig.

    abstract::The swine fatty acid binding protein gene (FABP3) has been screened with 4 primer sets for 270 purebred Yorkshire pigs from National Institute of Animal Science. The selection of primers was based on the swine cDNA sequence (AJ416019), and primer sets (FABP3-2 and FABP3-3) amplified target segments in real time PCR to...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0344-3

    authors: Cho KH,Kim MJ,Jeon GJ,Chung HY

    更新日期:2011-03-01 00:00:00

  • Optimizing mechanical stretching protocols for hypertrophic and anti-apoptotic responses in cardiomyocyte-like H9C2 cells.

    abstract::Cardiomyocytes possess the ability to respond to mechanical stimuli by reprogramming their gene expression. This study investigated the effects of different loading protocols on signaling and expression responses of myogenic, anabolic, inflammatory, atrophy and pro-apoptotic genes in cardiomyocyte-like H9C2 cells. Dif...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-06112-z

    authors: Zevolis E,Philippou A,Moustogiannis A,Chatzigeorgiou A,Koutsilieris M

    更新日期:2021-01-04 00:00:00

  • Development of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts.

    abstract::Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4329-y

    authors: Ascari IJ,Martins SC,Camargo LSA,Mendez-Otero R,Jasmin

    更新日期:2018-12-01 00:00:00

  • Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height.

    abstract::Seed weight and plant height are important agronomic traits and contribute to seed yield. The objective of this study was to identify QTLs underlying these traits using an intra-specific mapping population of chickpea. A F11 population of 177 recombinant inbred lines derived from a cross between SBD377 (100-seed weigh...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-015-3925-3

    authors: Gupta S,Kumar T,Verma S,Bharadwaj C,Bhatia S

    更新日期:2015-11-01 00:00:00

  • The polymorphisms of LYRM1 gene and their association with body measurement and ultrasound traits of Qinchuan cattle.

    abstract::Body measurement and meat quality traits which play important roles in the assessment of productivity and economy in cattle were influenced by genes and environmental factors. Latest studies showed that LYR motif containing 1 (LYRM1) may be involved in influencing fatness deposition in animals. The objective of this s...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2195-6

    authors: Li Y,Gao J,Yang N,Fu C,Cheng G,Wang H,Zan L

    更新日期:2013-02-01 00:00:00

  • Association of matrix metalloproteinase 3 and endogenous inhibitors with inflammatory markers in mitral valve disease and calcification.

    abstract::Calcific mitral valve stenosis (MVS) is a common disease characterized by extensive remodeling of the extracellular matrix via matrix metalloproteinases (MMPs). The mechanism of calcification due to extensive matrix remodeling remains unclear. In this study, we investigated the relationship between MMP-3, tissue inhib...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4372-8

    authors: Aloui S,Zidi W,Ouali S,Guizani I,Hadj-Taieb S,Mourali MS,Feki M,Allal-Elasmi M

    更新日期:2018-12-01 00:00:00

  • Evaluation of synthetic hexaploid wheats (derivative of durum wheats and Aegilops tauschii accessions) for studying genetic diversity using randomly amplified polymorphic DNA (RAPD) markers.

    abstract::Synthetic hexaploid (SH) wheat derived from crossing tetraploid durum wheat and diploid Aegilops tauschii provide germplasm for wheat improvement as the conventional wheat varieties possess very low genetic diversity. This study aims to identify diverse SH lines which can be used in breeding programs for transferring ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1943-y

    authors: Shakeel M,Ilyas M,Kazi M

    更新日期:2013-01-01 00:00:00

  • Denaturation of mouse satellite and ribosomal DNA during hydroxyapatite thermal chromatography of chromatin.

    abstract::Mouse DNA and chromatin were melted on hydroxyapatite and the denaturation profiles of ribosomal and satellite DNAs were followed by hybridization with their complementary RNAs. Neither ribosomal nor bulk DNA had significantly different melting profiles in chromatin as compared to DNA. However, most of satellite DNA e...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00368300

    authors: Pashev IG

    更新日期:1977-06-01 00:00:00

  • Study of molecular mechanism of Prostaglandin E1 in inhibiting coronary heart disease.

    abstract::Prostaglandin E1 has been used clinically for improving heart diseases. In this study, we examined the effect of Prostaglandin E1 on blood lipid levels, heart protein and genes expression in coronary heart disease (CHD) rats. Female rats were fed either a control diet or hypercholesterolemic diet for 14 weeks. The fee...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2785-y

    authors: Liu HJ,Ma JW,Qiao ZY,Xu B

    更新日期:2013-12-01 00:00:00

  • Molecular and morphological discrimination of Chrysanthemum indicum using allele-specific PCR and T-shaped trichome.

    abstract::Chrysanthemum indicum L. is a traditional oriental medicinal herb prepared as a tea from flowers that have been used in China and South Korea since ancient times. It has a long history in the treatment of hypertension, inflammation, and respiratory diseases. Among Chrysanthemum species, C. indicum has more active chem...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05844-2

    authors: Abid S,Kaliraj L,Arif MH,Hurh J,Ahn JC,Yang DC,Jung SK

    更新日期:2020-10-01 00:00:00

  • Multifactor dimensionality reduction analysis to elucidate the cross-talk between one-carbon and xenobiotic metabolic pathways in multi-disease models.

    abstract::Putatively functional polymorphisms of one-carbon and xenobiotic metabolic pathways influence susceptibility for wide spectrum of diseases. The current study was aimed to explore gene-gene interactions among these two metabolic pathways in four diseases i.e. breast cancer, systemic lupus erythematosus (SLE), coronary ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-015-3856-z

    authors: Naushad SM,Vijayalakshmi SV,Rupasree Y,Kumudini N,Sowganthika S,Naidu JV,Ramaiah MJ,Rao DN,Kutala VK

    更新日期:2015-07-01 00:00:00

  • Investigation of possible effects of microRNAs involved in regulation of lipid metabolism in the pathogenesis of atherosclerosis.

    abstract::Due to being stable in the circulatory system plasma miRNAs have been detected in various diseases including coronary artery disease (CAD) which is the major cause of mortality and morbidity worldwide. Atherosclerosis is the major cause of CAD. The first and most important event in the progression of atherosclerosis i...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4547-3

    authors: Gorur A,Celik A,Yildirim DD,Gundes A,Tamer L

    更新日期:2019-02-01 00:00:00

  • Impact of polymorphism of the regulatory subunit of the μ-calpain (CAPN1S) on the proteolysis process and meat tenderness of young cattle.

    abstract::The objective of this study was to estimate the impact of the polymorphism of μ-calpain (CAPN1S) gene on protein changes of the cattle muscle tissue and its tenderness during 10-day cold storage. The analysis was performed on the longest dorsal and lumbar muscles collected from 76 bulls 6 to 12 months of age. Polymorp...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0229-5

    authors: Iwanowska A,Grześ B,Mikołajczak B,Iwańska E,Juszczuk-Kubiak E,Rosochacki SJ,Pospiech E

    更新日期:2011-02-01 00:00:00