Abstract:
:J The [Ca2+] regulation of contractile ATPase flux, Jp, in skeletal muscle was analysed by computation of the Response R(Jp) Ca2+ for a 10 Hz range of electrical stimulation frequencies. Results of our analysis of the kinetic controls in ATP free energy metabolism in a network model of contracting muscle (J.A.L. Jeneson, H.V. Westerhoff and M.J. Kushmerick (2000) Am. J Physiol. 279, C813-C832) formed the basis for the computations of R(Jp) Ca2+. We found that neural regulation of sustained force generation via simple [Ca2+]cyto frequency encoding in the network was robust for frequencies up to 2 Hz. Above 2 Hz, however, this regulation design broke down because of a shift in contractile ATPase flux control from the Ca2+-sensitive contractile filaments to mitochondria with low Ca2+ sensitivity. The role of glyco(geno)lytic ATP production at high contraction workloads is discussed in the context of this result.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Jeneson JAdoi
10.1023/a:1020317610863keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
167-70issue
1-2eissn
0301-4851issn
1573-4978journal_volume
29pub_type
杂志文章abstract::Ebselen, an organoselenium compound, mimics glutathione peroxidase activity. It is a multifunctional compound, which catalyzes several essential reactions for the protection of cellular components from oxidative and free radical damage. Based on a number of in vitro and in vivo studies, various mechanisms are proposed...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3417-x
更新日期:2014-08-01 00:00:00
abstract::Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional requirements during periods of reproductive activity. Therefore, a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0666-1
更新日期:2011-11-01 00:00:00
abstract::A full length spermidine synthase (PgSPD) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The cDNA was 1,188 nucleotides long and had an open reading frame of 1,002 bp with a deduced amino acid sequence of 333 residues. The calculated molecular mass of the matured protein is approximate...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9725-x
更新日期:2010-02-01 00:00:00
abstract::Biochemical networks comprise many diverse components and interactions between them. It has intracellular signaling, metabolic and gene regulatory pathways which are highly integrated and whose responses are elicited by extracellular actions. Previous modeling techniques mostly consider each pathway independently with...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2153-3
更新日期:2013-02-01 00:00:00
abstract::45S ribosomal precursor RNA and large heterogeneous RNA molecules (greater than 45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments sedimenting at 28S a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00808381
更新日期:1977-12-01 00:00:00
abstract::Haemoglobin meseenger RNA was isolated from spleen erythroïd cells of anaemic rabbits. The mRNA fraction looks quite homogeneous when analyzed by sucrose gradient centrifugation; it sediments in the 9S region. When added to an ascites cell free system, spleen 9S RNA stimulates the incorporation of radioactive leucine ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00357405
更新日期:1973-06-01 00:00:00
abstract::Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats. We have mo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-006-9010-1
更新日期:2007-03-01 00:00:00
abstract::Long term intake of high-glucose diet (HGD) may induce many diseases such as dyslipidemia, fatty liver and diabetes disease. Most of the research for molecular mechanisms of the association between HGD and the above diseases focus on the metabolism of glucose and lipid. However, there are few studies on molecular mech...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9622-3
更新日期:2010-04-01 00:00:00
abstract::Hypertension is a risk factor for the cardiovascular diseases. Although, several drugs are used to treat hypertension, the success of the antihypertensive therapy is limited. Resveratrol decreases blood pressure in animal models of hypertension. This study researched the mechanisms behind the effects of resveratrol on...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3737-x
更新日期:2015-01-01 00:00:00
abstract::Adipose differentiation-related protein gene (ADP) plays an important role in controlling lipid accumulation in mammals. It may also affect lipid deposition in birds. However, the molecular mechanism of its actions in birds remains unknown. In the present study, the coding sequence of ADP cDNA for Chinese native breed...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9836-4
更新日期:2010-07-01 00:00:00
abstract::Genetic susceptibility and environment exposures are associated risk factors in carcinogenesis. Gene polymorphisms that decrease the activity of detoxifying carcinogen substances may modify the effect of exposures. We investigated whether the polymorphisms PON1 rs662 (Q192R), and PON1 rs854560 (L55M) would be associat...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3489-7
更新日期:2014-09-01 00:00:00
abstract::The q11.2 region on chromosome 22 contains numerous low-copy repeats that lead to deleted or duplicated regions in the chromosome, thereby resulting in different syndromes characterized by intellectual disabilities or congenital anomalies. The association between patient phenotypes and 22q11.2 copy number abnormalitie...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05815-7
更新日期:2020-10-01 00:00:00
abstract::The human dopamine transporter gene (hDAT) encodes the dopamine transporter in dopamine (DA) neurons to regulate DA transmission. hDAT expression varies significantly from neuron to neuron, and from individual to individual so that dysregulation of hDAT is related to many neuropsychiatric disorders. It is critical to ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1339-4
更新日期:2012-05-01 00:00:00
abstract::PNAS-4 is a novel pro-apoptotic protein activated during the early response to DNA damage; however, the molecular mechanisms and pathways regulating PNAS-4 expression in tumors are not well understood. We hypothesized that PNAS-4 is a p53 down-stream target gene and designed this study. We searched online for putative...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0732-3
更新日期:2012-01-01 00:00:00
abstract::Sheep are valuable resources for the wool industry. Wool growth of Aohan fine wool sheep has cycled during different seasons in 1 year. Therefore, identifying genes that control wool growth cycling might lead to ways for improving the quality and yield of fine wool. In this study, we employed Agilent sheep gene expres...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3405-1
更新日期:2014-08-01 00:00:00
abstract::Echinococcus granulosus is a parasitic helminth which affects both man and animals. During infection with larval stage of the organism secretory and membrane-bound (S/M) proteins play a meaningful role for evasion of immune system. Antigen B (AgB) is one of them. Present investigation has defined sequence diversity of...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0332-7
更新日期:2011-03-01 00:00:00
abstract::Familial hypercholesterolemia (FH) (OMIM 143890) is an autosomal dominantly inherited disease mainly caused by mutations of the gene encoding the low density lipoprotein receptor (LDLR) and Apolipoprotein (Apo) B. First the common mutation R3500Q in ApoB gene was determined using PCR/RFLP method. Then the LDLR gene wa...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9416-z
更新日期:2009-11-01 00:00:00
abstract::A nucleic acid sequence MC, encoding Momordica Chanrantia anti-hyperglycaemic peptide MC6 (accession: AAX06814) synthesized according to Escherichia coli preferred codons, was cloned and expressed in E. coli. Recombinant protein pQE8-MC (about 3.5 kDa) was purified and analyzed by 20% SDS-PAGE and western blot. It rev...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9609-0
更新日期:2010-04-01 00:00:00
abstract::Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). MicroRNAs (miRNAs) play an important role in plant defense modulation and therefore the analysis of these molecules can help better understand plant-pathogen ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04779-7
更新日期:2019-06-01 00:00:00
abstract::Nerve growth factor (NGF) is required for the differentiation and maintenance of sympathetic and sensory neurons. In the present study, the recombinant expression of human nerve growth factor beta (hNGF-β) gene in rabbit bone marrow mesenchymal stem cells (rMSCs) was undertaken. Recombinant vector containing hNGF-β wa...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0068-4
更新日期:2010-12-01 00:00:00
abstract::Xenin is a regulatory peptide first isolated from the human gastric mucosa. Using an open-access protein database MEDLINE (33 million molecules; 11 billion amino acid residues) and our original computer program, we conducted a search for the xenin motifs in the primary structure of proteins across almost the entire ta...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4147-2
更新日期:2018-04-01 00:00:00
abstract::Fusarium head blight (FHB), predominantly caused by Fusarium graminearum, is a destructive disease that poses a serious threat to wheat (Triticum aestivum L.) production around the world. A suppression subtractive hybridization (SSH) cDNA library was constructed from F. graminearum infected spikes of a resistant Belgi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1823-5
更新日期:2012-10-01 00:00:00
abstract::Dendropoma petraeum, considered the primary vermetid reef-building species in the Mediterranean, has recently been shown to be a species complex of at least four cryptic species. These species have highly restricted, non-overlapping distributions, causing concern for their conservation status. To better study the gene...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4346-x
更新日期:2018-12-01 00:00:00
abstract::Recently a GWAS study had identified 38 genomic variants commonly found in humans that influence migraine risk. For further replicate these findings, we selected two SNPs; rs2651899 on chromosome 1p36.32 in PRDM16 gene and rs10166942 on chromosome 2q37.1 close to TRPM8 gene for their associations with migraine in the ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04593-1
更新日期:2019-02-01 00:00:00
abstract::As an essential signaling modulator, Src gene appears to be necessary for increased expression of the prolactin receptor, normal downstream signaling, and alveolar cell organization. In this study, we detected the polymorphism of Src gene by polymerase chain reaction-restriction fragment length polymorphism assay (PCR...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0031-4
更新日期:2010-12-01 00:00:00
abstract::Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3633-4
更新日期:2014-11-01 00:00:00
abstract::The mechanism by which external Bax releases cytochrome c is still controversial and may also depend on the type of mitochondria and the actual localisation of cytochrome c. Outer membrane porin acquires high binding affinity for hexokinase by interacting with the adenine nucleotide translocator (ANT) in the contact s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1020383108620
更新日期:2002-01-01 00:00:00
abstract::HSG1 (Heat shock-induced gene 1) is one of the high-temperature-induced proteins in grapevine. Sequence analysis has demonstrated that HSG1 is a grape Bcl-2-associated athanogene (BAG) that contains IQ and BAG domains in its sequence. HSG1 expression was not detected in leaves without heat treatment, while tendrils, s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1224-1
更新日期:2012-04-01 00:00:00
abstract::Using a membrane filter retention technique we have studied the interaction between DNA and lysine rich histone H5 in vitro. It is found that, depending on the ionic conditions, H5 can bind DNA in a random or cooperative manner and exhibits a preference to DNA with high molecular weight and/or high A + T content, as a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775749
更新日期:1980-03-31 00:00:00
abstract::Methicillin resistant Staphylococcus aureus (MRSA) is one of the challenging bacterial pathogen due to its acquired resistance to the β lactam antibiotics. The Sortase A is an enzyme of Gram-positive bacteria including S. aureus to anchor surface proteins to the cell wall. Sortase A is well studied enzyme and consider...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3384-2
更新日期:2014-08-01 00:00:00