Abstract:
:The complete coding sequences of three porcine genes-SDHB, SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs. These three genes were then deposited into GenBank database and assigned to GeneID: 100125544, 768109 and 780429. The phylogenetic tree analysis revealed that the swine SDHB and SNRPA have closer genetic relationships with the human SDHB and SNRPA, but swine CRYBB1 has a closer genetic relationship with the bovine CRYBB1.The tissue expression analysis indicated that that swine SDHB, SNRPA and CRYBB1 gene were differentially expressed in tissues including fat, lung, muscle, small intestine, kidney, large intestine, spleen and liver. Our experiment is the first to establish the primary foundation for further research on these three swine genes.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
An QC,Liu GYdoi
10.1007/s11033-008-9229-0subject
Has Abstractpub_date
2009-04-01 00:00:00pages
683-90issue
4eissn
0301-4851issn
1573-4978journal_volume
36pub_type
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