Abstract:
:The aim of this study was to describe the microbial communities in the distal gut of wild wolves (Canis lupus). Fecal samples were collected from three healthy unrelated adult wolves captured at the nearby of Dalai Lake Nature Reserve in Inner Mongolia of China. The diversity of fecal bacteria was investigated by constructing PCR-amplified 16S rRNA gene clone libraries using the universal bacterial primers 27 F and 1493 R. A total of 307 non-chimeric near-full-length 16S rRNA gene sequences were analyzed and 65 non-redundant bacteria phylotypes (operational taxonomical units, OTUs) were identified. Seventeen OTUs (26%) showed less than 98% sequence similarity to 16S rRNA gene sequences were reported previously. Five different bacterial phyla were identified, with the majority of OTUs being classified within the phylum Firmicutes (60%), followed by Bacteroidetes (16.9%), Proteobacteria (9.2%), Fusobacteria (9.2%) and Actinobacteria (4.6%). The majority of clones fell within the order Clostridiales (53.8% of OTUs). It was predominantly affiliated with five families: Lachnospiraceae was the most diverse bacterial family in this order, followed by Ruminococcaceae, Clostridiaceae, Peptococcaceae and Peptostreptococcaceae.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Zhang H,Chen Ldoi
10.1007/s11033-010-0060-zsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
4013-22issue
8eissn
0301-4851issn
1573-4978journal_volume
37pub_type
杂志文章abstract::STAT4 is a transcription factor that has been implicated in the pathogenesis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Recently, several reports has documented that a STAT4 haplotype is associated with RA, SLE and Sjogren's syndrome. To summarize and review these findings, we conducted a met...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-009-9553-z
更新日期:2010-01-01 00:00:00
abstract::The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and coding regions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Compar...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0431-5
更新日期:2011-04-01 00:00:00
abstract::The majority of the genomic DNA sequence in mammalian and other higher organisms can be transcribed into abundant functional RNA transcripts, especially regulatory non-coding RNAs (ncRNAs) that are expressed in a developmentally and species-specific regulated manner. Here, we review various regulatory non-coding RNAs,...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3259-6
更新日期:2014-06-01 00:00:00
abstract::An RNA polymerase III transcript, 7SK nuclear RNA, was found to bear sequence homology to the B2 class of highly repeated elements in the mouse genome. Northern blot hybridizations between the small RNAs and two B2 clones showed that only one of them (p49C8) hybridized to 7SK RNA. Both clones, however, hybridized to 4...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00580650
更新日期:1987-01-01 00:00:00
abstract::Type 2 diabetes is one of the most prevalent diseases, which increases resistance to insulin in target tissues. The measurement of miRNAs quantity is a molecular approach for diagnosis of diabetes. miRNAs are small non-coding RNA strings of 21-23 long nucleotides that act as inhibitors in proteins translation. Several...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04959-5
更新日期:2019-10-01 00:00:00
abstract::The stem cells in freshwater flatworms (planarian) are called neoblasts. Neoblasts are capable of proliferation and differentiation into every cell type, including the gametes. For the investigation of the mechanisms of stem cells proliferation and differentiation the proper evaluation of changes in the cell cycle of ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1070-1
更新日期:2012-03-01 00:00:00
abstract::The complete mitochondrial genomes of five tiger samples from three subspecies (P. t. sumatrae, P. t. altica, and P. t. tigris) were successfully obtained by using 26 specifically designed Panthera-specific primer sets. The genome organization and gene arrangement of the five tiger samples were similar to each other; ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1384-z
更新日期:2012-05-01 00:00:00
abstract::Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low ex...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF01674434
更新日期:1993-10-01 00:00:00
abstract::Actins are ubiquitous and highly conserved proteins that play key roles in cell formation and cellular activities. In this study, an actin gene was isolated from chickpea for the first time and designated as CarACT1 (for Cicer arietinum L. actin gene 1; Genbank accession no. EU529707). It encoded a putative protein wi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9844-4
更新日期:2010-02-01 00:00:00
abstract::Stable inheritance and expression of transgene are important parameters for successful use of a transgenic crop. We previously transformed a Bt cry1Ba3 gene into cabbage inbred line CA21-3. To evaluate the stability of our Bt cabbage lineages, transgene inheritance and expression were examined in four successive gener...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05344-3
更新日期:2020-04-01 00:00:00
abstract::DNA repair capacity (DRC) can be altered based on sequence variations in DNA repair genes, which may result in cancer susceptibility. The current study was to evaluate the association between genetic polymorphisms, including associated haplotypes of xeroderma pigmentosum complementary group D (XPD), and individual sus...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0258-0
更新日期:2011-03-01 00:00:00
abstract::As a kind of binding protein, the type 1 Na(+)/H(+) exchanger (NHE1) is a receptor for the highly pathogenic Avian leukosis viruses-J subgroup (ALV-J) in chicken. In order to investigate the potential effect of chicken NHE1 gene on leukosis, we compared its expression between ALV-J-affected and -unaffected chicken, sc...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3535-5
更新日期:2014-10-01 00:00:00
abstract::ERBB2/HER2/NEU, a member of the epidermal growth factor receptor family, is overexpressed in more than 25 % of non-small cell lung cancer and is considered to be a significant and independent prognostic factor in lung cancer. Here we generated a lung specific HER2 overexpressing transgenic mouse model. In this model, ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1635-7
更新日期:2012-08-01 00:00:00
abstract::Synthetic hexaploid (SH) wheat derived from crossing tetraploid durum wheat and diploid Aegilops tauschii provide germplasm for wheat improvement as the conventional wheat varieties possess very low genetic diversity. This study aims to identify diverse SH lines which can be used in breeding programs for transferring ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1943-y
更新日期:2013-01-01 00:00:00
abstract::We report here a cDNA and its deduced amino acid sequence encoding a cathepsin D-like, aspartic protease from Chlamys farreri (denoted as CfCD) by expressed sequence tag and rapid amplification of cDNA ends techniques. The cDNA of CfCD consisted of 1,810 nucleotides with a canonical polyadenylation signal sequence AAT...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9534-2
更新日期:2010-03-01 00:00:00
abstract::Rheumatoid arthritis (RA) is considered as a long-term autoimmune disorder. Gene polymorphism and oxidative stress might be involved in the pathogenesis of the disease. We aimed to determine the association between PON-1L55M polymorphism and its effects on inflammatory markers such as anti-cytroline circulated-peptide...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4530-z
更新日期:2019-02-01 00:00:00
abstract::Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that plays an important role in breast cancer pathophysiology and risk definition, since it regulates the bioavailable fraction of circulating estradiol. Epidemiological studies have evaluated the association between SHBG Asp327Asn polymorphism and breast ca...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-1680-2
更新日期:2012-08-01 00:00:00
abstract::Nuclear magnetic resonance (NMR) spectroscopy is a useful biophysical technique to study the ligand-protein interaction. In this report, we have used bacterially produced ERβ and its domains for studying the functional analysis of ligand-protein interaction. Briefly, ERβ and its transactivation domain (TAD) and ligand...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0600-6
更新日期:2011-10-01 00:00:00
abstract::Cordyceps sinensis (CS) is an entomogenous fungus used as a tonic food and Chinese medicine to replenish health. This study investigated the protective effects of CS in rats post-renal ischemia-reperfusion (I/R) sequence by analyzing the influence on stromal cell-derived factor-1α (SDF-1α and chemokine (C-X-C motif) r...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2316-2
更新日期:2013-03-01 00:00:00
abstract::Responses of plants to salinity stress and the development of salt tolerance are extremely complex. Proteomics is a powerful technique to identify proteins associated with a particular environmental or developmental signal. We employed a proteomic approach to further understand the mechanism of plant responses to sali...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1460-z
更新日期:2012-05-01 00:00:00
abstract::Recently genetics and epigenetics alterations have been found to be characteristic of malignancy and hence can be used as targets for detection of neoplasia. RAS association domain family protein 1A (RASSF1A) gene hypermethylation has been a subject of interest in recent researches on cancer breast patients. The aim o...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2837-3
更新日期:2014-01-01 00:00:00
abstract::Vascular endothelial growth factor B (VEGF-B) was reported to be angiogenic, and it was considered as a neuroprotective agent in mouse retinal ganglion cells following optic nerve crush. Thus, it was necessary to investigate whether VEGF-B contributes to the process of retinal and choroidal neovascularization. We aime...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2917-4
更新日期:2014-02-01 00:00:00
abstract::It is well known that transcriptionally inactive rRNA genes are correlated with DNA hyper-methylation and histone hypo-methylation and there is clear evidence in humans that DNA and histone modification which alter chromatin structure are related to chromosome fragility. Very little is known about the biological cause...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2112-z
更新日期:2013-01-01 00:00:00
abstract::Chemerin, as a new member of adipokines family, is highly expressed in adipose tissue in rodent and its expression increases with obesity. Moreover, chemerin has been reported to have significant relationship with metabolic syndrome and insulin sensitivity. Here, the gene encoding chemerin from Xiang pig was cloned. T...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0934-8
更新日期:2012-02-01 00:00:00
abstract::Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-3002-8
更新日期:2014-03-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) has been investigated extensively at the molecular level. Nevertheless, genome wide study on the temporal and spatial dynamics of the virus is non-existed. To explore this topic, we determined complete coding genome sequences for four PRRSV isolates and analy...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2802-1
更新日期:2013-10-12 00:00:00
abstract::The aim of this study was to determine whether the Glutathione S-transferase M1 (GSTM1) and P1 (GSTP1) polymorphisms confer susceptibility to rheumatoid arthritis (RA). Meta-analysis was performed on the associations between the GSTM1 and GSTP1 null genotypes and RA, and on the association between smoking or seroposit...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-1965-5
更新日期:2012-12-01 00:00:00
abstract::The complete coding sequences of three porcine genes-SDHB, SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs. These three genes were then deposited into GenBa...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9229-0
更新日期:2009-04-01 00:00:00
abstract::Heterogeneous nuclear ribonucleoprotein particles (HnRNP) were separated in metrizamide density gradients, into two fractions migrating to 1.31 g ml-1 and 1.18 g ml-1, respectively. Proteins associated with each of these fractions were analysed by SDS-acrylamide gel electrophoresis. It is shown that the whole proteins...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00643481
更新日期:1977-03-01 00:00:00
abstract::The rapid progress made over the last 10 years in the identification of individual autoantigens and in the localization of the epitopes involved, has resulted in a parallel reduction in the complexity of the antigen required for the detection of autoantibodies. The ability to use synthetic peptides as antigens is a re...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00464709
更新日期:1992-06-01 00:00:00