Abstract:
:Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Sagong B,Bae JW,Rhyu MR,Kim UK,Ye MKdoi
10.1007/s11033-013-3002-8subject
Has Abstractpub_date
2014-03-01 00:00:00pages
1563-7issue
3eissn
0301-4851issn
1573-4978journal_volume
41pub_type
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