Multiplex minisequencing screening for PTC genotype associated with bitter taste perception.

Abstract:

:Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.

journal_name

Mol Biol Rep

authors

Sagong B,Bae JW,Rhyu MR,Kim UK,Ye MK

doi

10.1007/s11033-013-3002-8

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

1563-7

issue

3

eissn

0301-4851

issn

1573-4978

journal_volume

41

pub_type

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