Abstract:
:The circular dichroism spectra of poly(dG). poly(dC) have been studied as a function of pH in 0.15 M NaCl solution. Acid titration to pH 2.5 showed two transitions, one around pH 5, the second below pH 3. These transitions are disproportionation reactions and are not due to the dissociation of the complexes. Alkaline back titration to neutrality showed only one step above pH 7, without appearance of the intermediate form. The hysteresis loop observed gave rise to a metastable (probably protonated) form at pH 7 which reverted to the neutral form upon heating or alkali treatment. In order to obtain again the metastable hysteretic form, a whole titration cycle to pH 2.5 had to be performed.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Thiele D,Sarocchi MT,Guschlbauer W,Marck Cdoi
10.1007/BF00357155subject
Has Abstractpub_date
1973-10-01 00:00:00pages
149-54issue
3eissn
0301-4851issn
1573-4978journal_volume
1pub_type
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