Abstract:
:Transcriptional factors are the major regulators of plant signaling pathways in response to environmental stresses i.e., drought, salinity and cold. Hereby, the GhMYB108-like was characterized to determine whether it regulate these stresses. The GhMYB108-like cDNA consisted of 1107 base pairs (bp) with 807 open reading frame encoded a protein of 268 amino acids. Its isoelectric point and molecular weight are 5.51 and 30.3 kDa respectively. Phylogenetic analysis and online databases revealed that GhMYB108-like proteins are closely related with the Arabidopsis thaliana MYB2. Important cis-elements were detected in the promotor region of GhMYB108-like responding to stresses and phytohormones. The 3D structure of GhMYB108-like protein has been predicted. In addition, various physico-chemical properties of GhMYB108-like have been determined. Subcellular localization confirmed that GhMYB108-like are nuclear localized protein. Quantitative expression analysis showed that polyethylene glycol and salt treatments significantly induced the expression of GhMYB108-like. Overall, our findings suggest that GhMYB108-like is an important gene that would plays important regulatory role in response to drought and salt stresses.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Ullah A,Ul Qamar MT,Nisar M,Hazrat A,Rahim G,Khan AH,Hayat K,Ahmed S,Ali W,Khan A,Yang Xdoi
10.1007/s11033-020-05244-6subject
Has Abstractpub_date
2020-03-01 00:00:00pages
1573-1581issue
3eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-05244-6journal_volume
47pub_type
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