High-level expression of orange fluorescent protein in the silkworm larvae by the Bac-to-Bac system.

Abstract:

:This novel orange fluorescent protein (OFP) emits brilliant orange fluorescent light. OFP has high fluorescence quantum yield, fast maturation rate, and stability, which imply this protein should be the most favorable biotechnological tools used to investigate the function of target gene by visualizing, monitoring, and quantifying in living cells. B. mori, silkworm has been used as an important bioreactor for the production of recombinant proteins through baculovirus expression system (BES). In this paper, we used infection technique which introduced the baculovirus DNA into silkworms using a cationic lipofectin reagent instead of directly injecting the virus, and demonstrated a high-level expression of the orange fluorescent protein (OFP) gene in the Bombyx mori, silkworm larvae. When recombinant rBacmid/BmNPV/OFP DNA ranging from 50-100 ng/larval was injected, a sufficient OFP expression in hemolymph was harvested. The recombinant viruses could be obtained from the hemolymph of infected larvae and stored as seed which could be used for the large-scale expression. This procedure omitted the costly and labor-consumed insect cell culture. Further investigation of OFP should provide us with more insight in unlocking the mystery of the mechanisms of autocatalytic bioluminescence and its utilization in biotechnology.

journal_name

Mol Biol Rep

authors

Liu JM,David WC,Ip DT,Li XH,Li GL,Wu XF,Yue WF,Zhang CX,Miao YG

doi

10.1007/s11033-007-9183-2

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

329-35

issue

2

eissn

0301-4851

issn

1573-4978

journal_volume

36

pub_type

杂志文章
  • Expression analysis of turkey (Meleagris gallopavo) toll-like receptors and molecular characterization of avian specific TLR15.

    abstract::Toll-like receptors (TLRs) constitute a multi-gene family, which plays a pivotal role in sensing invading pathogens by virtue of conserved microbial patterns. TLR repertoire of chicken and zebra finch has been well studied. However TLR family of other avian species is yet to be characterized. In the present study, we ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1709-6

    authors: Ramasamy KT,Reddy MR,Verma PC,Murugesan S

    更新日期:2012-08-01 00:00:00

  • Association analysis, genetic diversity and structure analysis of tobacco based on AFLP markers.

    abstract::Knowledge in the area of genetic diversity could aid in providing useful information in the selection of material for breeding such as hybridization programs and quantitative trait loci mapping. To this end, 50 Nicotiana tabacum genotypes were genotyped with 21 primer combination of amplified fragment length polymorph...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3194-6

    authors: Dadras AR,Sabouri H,Nejad GM,Sabouri A,Shoai-Deylami M

    更新日期:2014-05-01 00:00:00

  • Adipose tissue mRNA expression of HDAC1, HDAC3 and HDAC9 in obese women in relation to obesity indices and insulin resistance.

    abstract::It is well-established that an impaired adipose tissue function and morphology caused by a dysregulated gene expression contribute substantially to obesity. Nowadays, animal model studies and in vitro surveys provide evidence for possible roles of HDACs as emerging epigenetic players in the pathogenesis of obesity. Ho...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05431-5

    authors: Jannat Ali Pour N,Meshkani R,Toolabi K,Mohassel Azadi S,Zand S,Emamgholipour S

    更新日期:2020-05-01 00:00:00

  • Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor.

    abstract::Latexin, a carboxypeptidase A inhibitor, is expressed in a cell type-specific manner in both central and peripheral nervous systems in the rat. It is used as a molecular marker for the regional specification of the neocortex. In this study, a cDNA was isolated from a human fetal brain cDNA library. The cDNA (LXN) cont...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1010971219806

    authors: Liu Q,Yu L,Gao J,Fu Q,Zhang J,Zhang P,Chen J,Zhao S

    更新日期:2000-01-01 00:00:00

  • Sulfadiazine binds and unfolds bovine serum albumin: an in vitro study.

    abstract::Sulfonamide derivatives, such as sulfadiazine (SD) are used as antibiotics and, very recently, anti-amyloid properties of these have been reported. We have evaluated binding of SD with bovine serum albumin (BSA) followed by unfolding of protein. Studies were accomplished at physiological conditions of temperature (37 ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2719-8

    authors: Al-Lohedan HA,Sajih Ali M

    更新日期:2013-11-01 00:00:00

  • Anesthetic ketamine counteracts repetitive mechanical stress-induced learning and memory impairment in developing mice.

    abstract::The aim of this study is to investigate whether ketamine, a noncompetitive N-methyl-D: -aspartate receptor (NMDAR) antagonist, had an influence on learning and memory in developing mice. Fifty Kunming mice aged 21 days were randomly divided into 5 subgroups (n = 10 for each) to receive intraperitoneal injection of equ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0561-9

    authors: Peng S,Zhang Y,Wang H,Ren B,Zhang J

    更新日期:2011-10-01 00:00:00

  • Targeted killing effects of double CD and TK suicide genes controlled by survivin promoter on gastric cancer cell.

    abstract::Suicide genes such as cytosine deaminase (CD) and herpes simplex virus thymidine kinase (TK) encode products that convert nontoxic substances (prodrugs) into toxic metabolites. Studies in recent years indicated that survivin(sur) expression was associated with the biological behaviors of gastric carcinoma. In the pres...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0218-8

    authors: Luo XR,Li JS,Niu Y,Miao L

    更新日期:2011-02-01 00:00:00

  • Association of Fas gene polymorphisms with systemic lupus erythematosus: a meta-analysis.

    abstract::The Fas gene polymorphisms -670A/G (rs1800682) and -1377G/A (rs2234767) have been shown to be associated with systemic lupus erythematosus (SLE), but findings are not consistent. To clarify this point, a meta-analysis was performed. We searched PubMed, CNKI, CBM and Wanfang database. Meta-odds ratios (ORs) and 95 % co...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-012-2075-0

    authors: Xiang N,Li XM,Wang GS,Tao JH,Li XP

    更新日期:2013-01-01 00:00:00

  • Hydrostatic and osmotic pressure study of the RNA hydration.

    abstract::The tertiary structure of nucleic acids results from an equilibrium between electrostatic interactions of phosphates, stacking interactions of bases, hydrogen bonds between polar atoms and water molecules. Water interactions with ribonucleic acid play a key role in its structure formation, stabilization and dynamics. ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1452-z

    authors: Giel-Pietraszuk M,Barciszewski J

    更新日期:2012-05-01 00:00:00

  • Computational analysis of human N-acetylgalactosamine-6-sulfate sulfatase enzyme: an update in genotype-phenotype correlation for Morquio A.

    abstract::Mucopolysaccharidosis IV A (MPS IV A) is a lysosomal storage disease produced by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme. Although genotype-phenotype correlations have been reported, these approaches have not enabled to establish a complete genotype-phenotype correlation, and they ha...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3383-3

    authors: Olarte-Avellaneda S,Rodríguez-López A,Alméciga-Díaz CJ,Barrera LA

    更新日期:2014-11-01 00:00:00

  • Gel based in vitro 3D model exploring the osteocytic potentiality of human CD34+ stem cells.

    abstract::Osteocytic potentiality of human CD34+ stem cells explored in the present study by generating in vitro agarose gel 3D model to understand the bone ossification process. The G-CSF and IL-3 mobilized human CD34+ stem cells isolated apheretically from donor peripheral blood and purity of the cells was assessed by FACS an...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-016-4053-4

    authors: Srikanth L,Sunitha MM,Kumar PS,Chandrasekhar C,Vengamma B,Sarma PV

    更新日期:2016-11-01 00:00:00

  • Different methylation patterns in BWS/SRS cases clarified by MS-MLPA.

    abstract::Molecular abnormalities in the 11p15.5 imprinted gene cluster lead to two different growth diseases: Beckwith-Wiedemann syndrome (BWS) and Silver-Russell syndrome (SRS). They are mainly caused by epigenetic alterations in one of the two imprinting 11p15 control regions (ICR1 and ICR2). These CpG-rich regions are diffe...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2057-2

    authors: Lukova M,Todorova A,Todorov T,Mitev V

    更新日期:2013-01-01 00:00:00

  • Difference of TGF-β/Smads signaling pathway in epithelial-mesenchymal transition of normal colonic epithelial cells induced by tumor-associated fibroblasts and colon cancer cells.

    abstract::Tumor microenvironment (TME) crucially functions in tumor initiation and progression. Stroma-tumor interactions and cellular transdifferentiation are the prerequisite for tumor formation. Transforming growth factor-β (TGF-β), a major cytokine secreted by tumor-associated fibroblasts (TAFs) and cancer cells, is a cruci...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04719-5

    authors: Wang XL,Huang C

    更新日期:2019-06-01 00:00:00

  • Towards an understanding of wheat chloroplasts: a methodical investigation of thylakoid proteome.

    abstract::We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This e...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1302-4

    authors: Kamal AH,Cho K,Komatsu S,Uozumi N,Choi JS,Woo SH

    更新日期:2012-05-01 00:00:00

  • The increased expression of DEC1 gene is related to HIF-1α protein in gastric cancer cell lines.

    abstract::Overexpression of differentiated embryo chondrocyte 1 (DEC1) has been reported to contribute to the cellular differentiation, proliferation, and apoptosis of various cancers. Our previous studies have shown that DEC1 was highly expressed in gastric cancer (GCa) tissues. However, there is no report about the expression...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1209-0

    authors: Zheng Y,Shi X,Wang M,Jia Y,Li B,Zhang Y,Liu Q,Wang Y

    更新日期:2012-04-01 00:00:00

  • Transcriptome profiling and molecular marker discovery in red pepper, Capsicum annuum L. TF68.

    abstract::Transcriptome from high throughput sequencing-by-synthesis is a good resource of molecular markers. In this study, we present utility of massively parallel sequencing by synthesis for profiling the transcriptome of red pepper (Capsicum annuum L. TF68) using 454 GS-FLX pyrosequencing. Through the generation of approxim...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1102-x

    authors: Lu FH,Cho MC,Park YJ

    更新日期:2012-03-01 00:00:00

  • Profiling methyl-CpG specific determinants on transcriptionally silent chromatin.

    abstract::Transcriptional activity is closely associated with DNA methylation and chromatin remodelling. Evidence is emerging that a family of methylation specific (methyl-CpG binding domain, MBD) proteins have the capacity to bind to methylated sequences and repress transcription. Recent advances in this area reveal that many ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1015744625049

    authors: El-Osta A,Baker EK,Wolffe AP

    更新日期:2001-01-01 00:00:00

  • Diversity of bacterial communities related to the nitrogen cycle in a coastal tropical bay.

    abstract::A culture-independent molecular phylogenetic analysis was carried out to study for the first time the diversity of bacterial ammonia monooxygenase subunit A (amoA) and nitrogenase reductase subunit H (nifH) genes from Urca inlet at Guanabara Bay in Rio de Janeiro, Brazil. Most bacterial amoA and nifH sequences exhibit...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1111-9

    authors: Gonzalez AM,Vieira RP,Cardoso AM,Clementino MM,Albano RM,Mendonça-Hagler L,Martins OB,Paranhos R

    更新日期:2012-04-01 00:00:00

  • Immunity augmentation in Botia dario (Hamilton, 1822) through carotenoid: a dietary approach.

    abstract::The immunity and health status of ornamental fish is an important aspect, as they are kept in a confined environment and various stressful conditions which lead to depletion of overall colourful appearance and mortality. The carotenoids can act as immunity boosters in captive aquarium system and may be supplemented in...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05027-8

    authors: Tripathy PS,Khatei A,Parhi J,Choudhury TG,Sahoo L,Mandal SC

    更新日期:2019-12-01 00:00:00

  • Characterization of a F-box gene up-regulated by phytohormones and upon biotic and abiotic stresses in grapevine.

    abstract::F-box proteins are key components of the ubiquitin (Ub)/26S proteasome pathway that mediates selective degradation of regulatory proteins involved in a wide variety of cellular processes affecting eukaryotic cells. In plants, F-box genes form one of the largest multigene superfamilies and control many important biolog...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0438-y

    authors: Paquis S,Mazeyrat-Gourbeyre F,Fernandez O,Crouzet J,Clément C,Baillieul F,Dorey S

    更新日期:2011-06-01 00:00:00

  • Identification of a transforming growth factor-β type I receptor transcript in Eriocheir sinensis and its molting-related expression in muscle tissues.

    abstract::The transforming growth factor-β (TGF-β) signaling pathway is conserved across animals, and knowledge of its roles during the molt cycle in crustaceans is presently very limited. This study investigates the roles of the TGF-β receptor in molting-related muscle growth in Eriocheir sinensis. Using the RT-PCR and RACE te...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05108-8

    authors: Tian Z,Peng H,Deng W,Jiao C

    更新日期:2020-01-01 00:00:00

  • Molecular characterization and genetic structure of Quercus acutissima germplasm in China using microsatellites.

    abstract::Quercus acutissima is native to eastern Asia. It has a wide distribution in China and China is an important component in understanding the ecology and genetic structure of this species. Q. acutissima attained high economic value for hardwood product and can be managed as an energy tree species. To investigate the gene...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2486-6

    authors: Zhang YY,Fang YM,Yu MK,Li XX,Xia T

    更新日期:2013-06-01 00:00:00

  • Mutagenesis of tyrosine residues within helix VII in subunit I of the cytochrome cbb₃ oxidase from Rhodobacter capsulatus.

    abstract::The cbb (3)-type oxidases are members of the heme-copper oxidase superfamily, distant by sequence comparisons, but sharing common functional characteristics. The cbb (3) oxidases are missing an active-site tyrosine residue that is absolutely conserved in all A and B-type heme-copper oxidases. This tyrosine is known to...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0437-z

    authors: Oztürk M,Watmough NJ

    更新日期:2011-06-01 00:00:00

  • Structure and structure formation of the 20S proteasome.

    abstract::Eukaryotic 20S proteasomes are complex oligomeric proteins. The maturation process of the 14 different alpha- and beta-subunits has to occur in a highly coordinate manner. In addition beta-subunits are synthesized as proproteins and correct processing has to be guaranteed during complex maturation. The structure forma...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006826725056

    authors: Schmidt M,Schmidtke G,Kloetzel PM

    更新日期:1997-03-01 00:00:00

  • Identification and characterization of SSRs from soybean (Glycine max) ESTs.

    abstract::Microsatellites, or simple sequence repeats (SSRs), are very useful molecular markers for a number of plant species. We used a new publicly available module (TROLL) to extract microsatellites from the public database of soybean expressed sequence tag (EST) sequences. A total of 12,833 sequences containing di- to penta...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-1776-8

    authors: Xin D,Sun J,Wang J,Jiang H,Hu G,Liu C,Chen Q

    更新日期:2012-09-01 00:00:00

  • Molecular analysis of an actin gene, CarACT1, from chickpea (Cicer arietinum L.).

    abstract::Actins are ubiquitous and highly conserved proteins that play key roles in cell formation and cellular activities. In this study, an actin gene was isolated from chickpea for the first time and designated as CarACT1 (for Cicer arietinum L. actin gene 1; Genbank accession no. EU529707). It encoded a putative protein wi...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-009-9844-4

    authors: Peng H,Cheng H,Yu X,Shi Q,Zhang H,Li J,Ma H

    更新日期:2010-02-01 00:00:00

  • The drug-transporter gene MDR1 C3435T and G2677T/A polymorphisms and the risk of multidrug-resistant epilepsy in Turkish children.

    abstract::One-third of all individuals with epilepsy are resistant to antiepileptic drug (AED) treatment. Antiepileptic treatment response has been suggested to be modulated by genetic polymorphisms of drug efflux transporters. Several polymorphic variants within the multidrug resistance 1 (MDR1) gene, which encodes the major t...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-013-2866-y

    authors: Seven M,Batar B,Unal S,Yesil G,Yuksel A,Guven M

    更新日期:2014-01-01 00:00:00

  • Role of the ubiquitin-proteasome pathway in sepsis-induced muscle catabolism.

    abstract::Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006916206260

    authors: Hasselgren PO

    更新日期:1999-04-01 00:00:00

  • RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.

    abstract::Methyltransferases play essential roles in modulating important cellular and metabolic processes. A mouse putative N6-DNA methyltransferase gene (GenBank No AY456393) is a novel gene named mN6amt1(mN6A1). To investigate its function in cell fate and protein translation, RNA interference (RNAi)-mediated knock-down meth...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-008-9243-2

    authors: Liu Y,Nie D,Huang Y,Lu G

    更新日期:2009-04-01 00:00:00

  • Proangiogenic signature in advanced laryngeal carcinoma after microRNA expression profiling.

    abstract::The aim of this study is to evaluate which dysregulated angiomiRs compose the specific proangiogenic microRNA signature of advanced laryngeal cancer and review the literature. Thirty-six samples from twelve patients with advanced laryngeal carcinoma were collected. Total RNA was extracted and microRNA global profiling...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05250-8

    authors: Popov TM,Giragosyan S,Petkova V,Stancheva G,Marinov T,Belitova M,Rangachev J,Popova D,Kaneva RP

    更新日期:2020-07-01 00:00:00