Abstract:
:Despite advantageous antitumor properties of doxorubicin, the considerable cytotoxicity of this chemotherapeutic agent has made it necessary to develop combination treatment strategies. The aim of the current study was to investigate the possible synergism between dendrosomal nanocurcumin (DNC) and doxorubicin in eliciting anticancer effects on MDA-MB-231 metastatic breast cancer cells. The expression levels of CXCL12/CXCR4 axis and Hedgehog pathway genes were evaluated in patient-derived breast carcinoma tissues by qRT-PCR. MTT assay, Annexin V-FITC staining followed by flowcytomety and wound healing assay were used to measure the effects caused by DNC and doxorubicin, alone and in combination, on the viability, apoptosis induction, and migration of MDA-MB-231 cells, respectively. Also, qRT-PCR was exploited to analyze the expression of Smo, NF-κB and CXCR4 in cancer cells. Our results revealed that combination treatment with DNC and doxorubicin leads to significantly decreased viability, increased apoptosis, and reduced migration of breast cancer cells compared with using each drug alone. Also, combination treatment is more efficient that single treatment in reducing the expression levels of NF-κB and Smo transcripts. Our findings provide convincing support for the notion that DNC could synergistically enhance the anticancer effects of doxorubicin on metastatic breast cancer cells by improving its anti-proliferative, pro-apoptotic, and anti-migratory activities. This may be mediated, in part, by downregulating CXCR4, NF-κB, and Smo genes. Overall, the findings of the current study suggest that DNC might be used as a synergistic agent for enhancing therapeutic efficiency and reducing toxic effects of doxorubicin on breast cancer cells.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Mahjoub MA,Bakhshinejad B,Sadeghizadeh M,Babashah Sdoi
10.1007/s11033-017-4115-2subject
Has Abstractpub_date
2017-08-01 00:00:00pages
341-351issue
4eissn
0301-4851issn
1573-4978pii
10.1007/s11033-017-4115-2journal_volume
44pub_type
杂志文章abstract::Methylenetetrahydrofolate reductase (MTHFR) polymorphism may play an important role in the pathophysiology of obesity and diabetes accompanied by obesity due to its influence on plasma homocysteine levels. There are significant and sometimes very strong relationship between levels of homocysteine and several multi-sys...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9545-z
更新日期:2010-01-01 00:00:00
abstract::Several mechanisms are involved in the loss of cellular integrity and tissue destructions in various brain regions during ischemic insult. The affected brain employs various self-repair mechanisms during the poststroke recovery. Therefore, the current study involves time course changes in different brain regions follo...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05060-7
更新日期:2019-12-01 00:00:00
abstract::Pathologic complete response after neoadjuvant systemic treatment appears to be a valid surrogate for better overall survival in breast cancer patients. Currently, together with standard clinicopathologic assessment, novel molecular biomarkers are being exhaustively tested in order to look into the heterogeneity of br...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1576-1
更新日期:2012-07-01 00:00:00
abstract::Lung cancer is the most common cause of cancer death worldwide. Smoking is known as the strongest single factor in the development of lung cancer. However, there are inherited genetic factors that cause different responses to cigarette smoking exposure among individuals. We tried to identify these differences in heavy...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0672-3
更新日期:2011-11-01 00:00:00
abstract::The complete mitochondrial genome 15,650 bp in size of the Deracantha onos has been determined. The gene content, base composition and codon usage of D. onos are coincident to typical hexapods mitochondrial genomes. Genes arrangement of D. onos is identical to Gryllotalpa orientalis, Ruspolia dubia and Anabrus simplex...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9145-8
更新日期:2009-01-01 00:00:00
abstract::The present study brings out the preventive role of (-)-epigallocatechin-gallate (EGCG) on cardiac mitochondrial metabolism and apoptosis in cigarette smoke (CS)-exposed rats. The CS-exposed rats showed significantly decreased activities of TCA cycle enzymes and mitochondrial enzymatic antioxidants, on the other hand,...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2673-5
更新日期:2013-12-01 00:00:00
abstract::Bone abnormalities as a consequence of osteoblast deregulation are associated with several diseases such as diabetes and chronic kidney disease. Important role for oxidized low density lipoproteins (oxLDL) in the pathophysiology of bone disorders has been reported. However, little is known about the effects and mechan...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04735-5
更新日期:2019-06-01 00:00:00
abstract::Many studies have been performed to identify regulatory circuit underlying plant stress tolerance. However, the reliability of some findings has been criticized because of exclusive use of stress sensitive plant species such as Arabidopsis thaliana. Sensitive plant species often harbor narrow defensive mechanisms and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4262-0
更新日期:2018-10-01 00:00:00
abstract::Oryza rufipogon L. (O. rufipogon) or a common wild rice, showed considerable aluminum (Al) tolerance. In this study, we examined the physiologic and genetic response of wild rice short term and long term to Al toxicity, respectively. In the short term study, morin staining, DAPI staining and aniline blue staining were...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0618-9
更新日期:2011-11-01 00:00:00
abstract::Recently a GWAS study had identified 38 genomic variants commonly found in humans that influence migraine risk. For further replicate these findings, we selected two SNPs; rs2651899 on chromosome 1p36.32 in PRDM16 gene and rs10166942 on chromosome 2q37.1 close to TRPM8 gene for their associations with migraine in the ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04593-1
更新日期:2019-02-01 00:00:00
abstract::Interleukin 15 (IL-15) is a Th1-related cytokine that triggers inflammatory cell recruitment with implications for pathogenesis in ulcerative colitis. The IL-15 gene is located within a 35 kb region of the q28-31 locus of chromosome 4. In the present work, the role of IL-15 gene polymorphisms as susceptibility markers...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3067-z
更新日期:2014-01-01 00:00:00
abstract::Mucopolysaccharidosis IV A (MPS IV A) is a lysosomal storage disease produced by the deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) enzyme. Although genotype-phenotype correlations have been reported, these approaches have not enabled to establish a complete genotype-phenotype correlation, and they ha...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3383-3
更新日期:2014-11-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) has been investigated extensively at the molecular level. Nevertheless, genome wide study on the temporal and spatial dynamics of the virus is non-existed. To explore this topic, we determined complete coding genome sequences for four PRRSV isolates and analy...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2802-1
更新日期:2013-10-12 00:00:00
abstract::The plasticity of the central nervous system helps form the basis for the neurobiology of learning and memory. Long-term potentiation (LTP) is the main form of synaptic plasticity, reflecting the activity level of the synaptic information storage process, and provides a good model to study the underlying mechanisms of...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-010-0128-9
更新日期:2011-01-01 00:00:00
abstract::It has been about nine decades since the proposal of Otto Warburg on the metabolism of cancer cells. Unlike normal cells which undergo glycolysis and oxidative phosphorylation in the presence of oxygen, proliferating and cancer cells exhibit an increased uptake of glucose and increased rate of glycolysis and predomina...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-014-3764-7
更新日期:2015-04-01 00:00:00
abstract::Low levels of coenzyme Q10 (CoQ10) have been reported in the circulation of patients with breast cancer, particularly in metastatic features. Our objective was to study the correlation between plasma levels of CoQ10 and the tumoral expression levels of AMPK, PFKFB3, VEGF, and VEGFR2. This study was a part of consecuti...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05310-z
更新日期:2020-04-01 00:00:00
abstract::Using a membrane filter retention technique we have studied the interaction between DNA and lysine rich histone H5 in vitro. It is found that, depending on the ionic conditions, H5 can bind DNA in a random or cooperative manner and exhibits a preference to DNA with high molecular weight and/or high A + T content, as a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00775749
更新日期:1980-03-31 00:00:00
abstract::The purpose of this study was to assess the value of cytokeratin 19 (CK19) and matrix metalloproteinase 2 (MMP-2) in predicting lymph node metastasis (LNM) and survival after curative resection in hepatocellular carcinoma (HCC) patients. Expression of CK19 and MMP-2 in tumor tissue was assessed through immunohistochem...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0463-x
更新日期:2011-06-01 00:00:00
abstract::Imprinted genes play important roles in mammalian growth, development and behavior. In this study, we obtained 1568 bp mRNA sequence of porcine DIO3 (deiodinase, iodothyronine, type III), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 278 amin...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0983-z
更新日期:2012-03-01 00:00:00
abstract::Fenpropathrin (FEN) is a pyrethroid insecticide. Mammals can be exposed to these compounds with food and water as non-target organisms. Pyrethroids are classified into two types depending on chemical structure and neurotoxic effects. FEN has features of Type I and Type II pyrethroids. There is data that pyrethroids ap...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05480-w
更新日期:2020-06-01 00:00:00
abstract::Expressed sequence tags (ESTs) provide valuable tools that can be used to predict the genes involved in primary and secondary metabolite synthesis. To the best of our knowledge, ESTs have not yet been developed for Codonopsis. lanceolata, and therefore, the EST referenced in this report is the first transcript for C. ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0464-9
更新日期:2011-06-01 00:00:00
abstract::The spleen plays a crucial role in innate and adaptive immunity in bony fish. Consequently, this organ is well suited to assess the immune competence of an organism and hence provides useful information for comparison and classification of production traits of food fish, such as robustness and susceptibility. To gain ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2252-1
更新日期:2013-02-01 00:00:00
abstract::Accurate normalization of gene expression with qRT-PCR depends on the use of appropriate reference genes (RGs) for the species under a given set of experimental conditions. Multiple RGs for gene expression analysis of soybean exposed to heavy metal stress treatment have not been reported in the literature. In this stu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0897-9
更新日期:2012-02-01 00:00:00
abstract::An RNA polymerase III transcript, 7SK nuclear RNA, was found to bear sequence homology to the B2 class of highly repeated elements in the mouse genome. Northern blot hybridizations between the small RNAs and two B2 clones showed that only one of them (p49C8) hybridized to 7SK RNA. Both clones, however, hybridized to 4...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00580650
更新日期:1987-01-01 00:00:00
abstract::Rheumatic heart disease (RHD) or acute rheumatic fever (ARF) develops as a consequence of an exaggerated immune response to Group A beta haemolytic streptococci causing pharyngitis. The molecular mimicry appears between human cardiac myosin and M protein of group A streptococcal membranes. The polymorphism of the prot...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0692-7
更新日期:2011-11-01 00:00:00
abstract::J The [Ca2+] regulation of contractile ATPase flux, Jp, in skeletal muscle was analysed by computation of the Response R(Jp) Ca2+ for a 10 Hz range of electrical stimulation frequencies. Results of our analysis of the kinetic controls in ATP free energy metabolism in a network model of contracting muscle (J.A.L. Jenes...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1020317610863
更新日期:2002-01-01 00:00:00
abstract::The aim of this study was to explore the association between alpha-thalassemia, fetal hemoglobin, hematological indices, and clinical adverse events in Angolan sickle cell disease pediatric patients. A total of 200 sickle cell disease (SCD) children were sampled in Luanda and Caxito. A venous blood sample was collecte...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05628-8
更新日期:2020-07-01 00:00:00
abstract::Circular RNA (circRNA), a novel type of non-coding RNA that consists of a circular loop, has been demonstrated to act as a "sponge" for microRNAs (miRNAs). However, the role of circRNAs in keloid remains unknown. In this study, we investigated circRNA expression profiles in keloid to identify potential diagnostic and ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05120-y
更新日期:2020-01-01 00:00:00
abstract::The differentially expressed genes in the chickpea pod wall have been identified for the first time using a forward suppression subtractive hybridization (SSH) library. In all, 226 clones of SSH library were sequenced and analyzed. A total of 179 high-quality expressed sequence tags (ESTs) were generated and based on ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-05005-0
更新日期:2019-12-01 00:00:00
abstract::In autoimmune disease body's own immune system knows healthy cells as undesired and foreign cells. Over 80 types of autoimmune diseases have been recognized. Currently, at clinical practice, treatment strategies for autoimmune disorders are based on relieving symptoms and preventing difficulties. In other words, there...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/s11033-019-04588-y
更新日期:2019-02-01 00:00:00