Abstract：
:Plant microRNAs have shown the capacity to regulate mammalian systems. The potential bioactivity of miR171vr, an isoform of the plant miR171, on human embryonic kidney 293 (HEK293) cells was investigated. Bioinformatics simulations revealed that human G protein subunit alpha 12 (GNA12) transcript could represent an excellent target for miR171vr. To confirm this prediction, in vitro experiments were performed using a synthetic microRNA designed on miR171vr sequence. MiR-treated cells showed a significant decrease of GNA12 mRNA and protein levels, confirming the putative cross-kingdom interaction. In addition, miR171vr determined the modulation of GNA12 downstream signaling factors, including mTOR, as expected. Finally, the effect of the plant miRNA on HEK293 cell growth and its stability in presence of several stressors, such as those miming digestive processes and procedures for preparing food, were evaluated. All this preliminary evidence would suggest that miR171vr, introduced by diet or as supplement in gene therapies, could potentially influence human gene expression, especially for treating disorders where GNA12 is over-expressed (i.e. oral cancer, breast and prostate adenocarcinoma) or mTOR kinase is down-regulated (e.g. obesity, type 2 diabetes, neurodegeneration).
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Gismondi A,Nanni V,Monteleone V,Colao C,Di Marco G,Canini Adoi
10.1007/s11033-020-06070-6subject
Has Abstractpub_date
2021-01-01 00:00:00eissn
0301-4851issn
1573-4978pii
10.1007/s11033-020-06070-6pub_type
杂志文章abstract：:The current study represents the first molecular characterization of freshwater fish species in Egypt from two major fish resources; the River Nile and Lake Nasser. A total of 160 DNA barcodes using a 655-bp-long fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene were generated from 37 species belon...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05657-3
更新日期：2020-08-01 00:00:00
abstract：:Ethylene responsive factor (ERF) proteins play important roles in plant growth and development and regulate biotic and abiotic stress responses. In this study, a full length mRNA encoding a novel ERF-type transcription factor namely Ceratoides arborescens ERF protein (CeERF) was isolated from C. arborescens. The deduc...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0869-0
更新日期：2012-02-01 00:00:00
abstract：:The purpose of this study was to assess the value of cytokeratin 19 (CK19) and matrix metalloproteinase 2 (MMP-2) in predicting lymph node metastasis (LNM) and survival after curative resection in hepatocellular carcinoma (HCC) patients. Expression of CK19 and MMP-2 in tumor tissue was assessed through immunohistochem...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0463-x
更新日期：2011-06-01 00:00:00
abstract：:Matrix metalloproteinases (MMPs) are implicated in atherosclerosis evolution into a coronary artery disease (CAD). They could be used as biomarkers for a predictive approach when they are studied simultaneously. We aim in our study to demonstrate prospectively in patients with history of CAD that MMPs level is linked ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04914-4
更新日期：2019-10-01 00:00:00
abstract：:The GRAS gene family is a family of transcription factors that regulates plant growth and development. Despite being well-studied in many plant species, little is known about this gene family in orchardgrass (Dactylis glomerata L.), one of the top four economically important perennial forage grasses cultivated worldwi...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05279-9
更新日期：2020-03-01 00:00:00
abstract：:TCF7L2 and SLC30A8 have been found to be associated with type 2 diabetes mellitus (T2DM) as well as with impaired proinsulin processing recently, enzymes encoded by PCSK1 and PCSK2 are reported to play an important role in the process of proinsulin conversion. To investigate whether the single nucleotide polymorphisms...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0705-6
更新日期：2012-01-01 00:00:00
abstract：:Two DREB-like genes, designated as DmDREBa and DmDREBb, were isolated from chrysanthemum by RACE approach. The DmDREBa and DmDREBb genes encoded proteins of 191 and 185 amino acid residues with the predicted molecular mass of 21.66 and 20.99 kDa, respectively. Both DmDREBs proteins comprised a typical EREBP/AP2 domain...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9153-8
更新日期：2009-01-01 00:00:00
abstract：:The tertiary structure of nucleic acids results from an equilibrium between electrostatic interactions of phosphates, stacking interactions of bases, hydrogen bonds between polar atoms and water molecules. Water interactions with ribonucleic acid play a key role in its structure formation, stabilization and dynamics. ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1452-z
更新日期：2012-05-01 00:00:00
abstract：:Yellow lupin seeds (Lupinus luteus) cytoplasmic tRNAGlu was isolated and the primary structure was determined to be: (sequence in text) AGU CCCGGCGACGGAACCAOH. It is 76 nucleotides long and contains 8 modified nucleosides: 2 residues of pseudouridine, ribothymidine, 3 dihydrouridines, 5-methylcytosine and 1-methyladen...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00805633
更新日期：1988-01-01 00:00:00
abstract：:1-Deoxynojirimycin (DNJ), the main alkaloid in mulberry leaves, was recognized to treat patients with type 2 diabetes mellitus (T2DM). However, the regulatory mechanism of DNJ on glucose homeostasis was still unclear. In the present study, a safe concentration of 0.1-10 μmol/L for DNJ was incubated with mature 3T3-L1 ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05069-y
更新日期：2019-12-01 00:00:00
abstract：:The swine fatty acid binding protein gene (FABP3) has been screened with 4 primer sets for 270 purebred Yorkshire pigs from National Institute of Animal Science. The selection of primers was based on the swine cDNA sequence (AJ416019), and primer sets (FABP3-2 and FABP3-3) amplified target segments in real time PCR to...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0344-3
更新日期：2011-03-01 00:00:00
abstract：:A culture-independent molecular phylogenetic analysis was carried out to study for the first time the diversity of bacterial ammonia monooxygenase subunit A (amoA) and nitrogenase reductase subunit H (nifH) genes from Urca inlet at Guanabara Bay in Rio de Janeiro, Brazil. Most bacterial amoA and nifH sequences exhibit...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1111-9
更新日期：2012-04-01 00:00:00
abstract：:It is widely accepted that protein phosphorylation is a major control event in regulating cell cycle. In the present study, a novel M-phase phosphoprotein 6 (MPP6) was identified from black tiger shrimp Penaeus monodon (designated as PmMPP6) by cDNA library and RACE approaches. The full-length cDNA of PmMPP6 was of 69...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-2173-z
更新日期：2013-02-01 00:00:00
abstract：:The increasing worldwide incidence of mycobacteriosis and the need to achieve improved clinical management makes nontuberculous mycobacteria (NTM) genotyping a useful tool. However, because of technical difficulties, medium size microbiology laboratories do not attempt to compare the genetic patterns that each of thei...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05420-8
更新日期：2020-05-01 00:00:00
abstract：:Dyggve melchior clausen syndrome (DMC, MIM 223800) is a very rare autosomal recessive form of skeletal dysplasia associated with various degrees of mental retardation. It is characterized by a progressive spondyloepimetaphyseal dysplasia (SEMD) with disproportionate short stature, generalized platyspondyly and lacy il...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05774-z
更新日期：2020-09-01 00:00:00
abstract：:The aim of this study was to describe the microbial communities in the distal gut of wild wolves (Canis lupus). Fecal samples were collected from three healthy unrelated adult wolves captured at the nearby of Dalai Lake Nature Reserve in Inner Mongolia of China. The diversity of fecal bacteria was investigated by cons...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0060-z
更新日期：2010-12-01 00:00:00
abstract：:Intramembrane proteases control many important processes in a wide variety of organisms through regulated intramembrane proteolysis (RIP). However, very few intramembrane proteases have been characterized in plants. Intriguingly, EGY2 in Arabidopsis belongs to the Site-2 protease (S2P) family that performs RIP. It con...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0962-4
更新日期：2012-03-01 00:00:00
abstract：:In a meritorious effort H. de Rothschild compiled in 1899 all publications on mammary gland development and milk--a grand total of 8375 [1]. In the preface to this publication Duclaux states: 'Such a discrepancy between the tremendous efforts and the paltriness of the results--hundreds of scientists and thousands of r...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1023/a:1006851531360
更新日期：1997-08-01 00:00:00
abstract：:Osteocytic potentiality of human CD34+ stem cells explored in the present study by generating in vitro agarose gel 3D model to understand the bone ossification process. The G-CSF and IL-3 mobilized human CD34+ stem cells isolated apheretically from donor peripheral blood and purity of the cells was assessed by FACS an...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-4053-4
更新日期：2016-11-01 00:00:00
abstract：:The genes of E. coli are located on a circular chromosome of 4.6 million basepairs. This 1.6 mm long molecule is compressed into a nucleoid to fit inside the 1-2 microm cell in a functional format. To examine the role of DNA supercoiling as nucleoid compaction force we modulated the activity of DNA gyrase by electroni...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1020318705894
更新日期：2002-01-01 00:00:00
abstract：:A full-length cDNA encoding common bean (Phaseolus vulgaris L.) sucrose synthase (designated as Pv_BAT93 Sus), which catalyses the synthesis and cleavage of sucrose, was isolated from seeds at 15 days after pollination (DAP) by rapid amplification of cDNA ends (RACE). The full-length cDNA of Pv_BAT93 Sus had a 2,418 b...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0842-y
更新日期：2012-02-01 00:00:00
abstract：:PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognitio...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/BF00778522
更新日期：1985-04-01 00:00:00
abstract：:Brucella cell surface protein (BCSP31) is potentially useful for diagnosing brucellosis. We aimed to establish a monoclonal antibody (MAb) against Brucella melitensis BCSP31 and to investigate its distribution in diagnosis. Soluble recombinant BCSP31 was successfully expressed and purified. Two MAbs (1F1 and 1E5) agai...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0755-9
更新日期：2012-01-01 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) in adiponectin gene [rs1501299 (+276G/T) and rs266729 (-11377C/G)] and one SNP of leptin gene [rs7799039 (-2548G/A)] are known to influence plasma levels of adiponectin and leptin respectively. Literature is scarce on the association of adiponectin gene polymorphism rs266729 with...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05070-5
更新日期：2019-12-01 00:00:00
abstract：:Coactivator CBP (CREB-binding protein) has been implicated in the regulation of transcription for all signal transducer and activator of transcription factors (STATs); however, the mechanism remains unclear. Using yeast two-hybrid screening and immunoprecipitation techniques, we investigated the direct interaction of ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0622-0
更新日期：2011-10-01 00:00:00
abstract：:The suppressors of cytokine signaling (SOCS) proteins are originally identified as negative regulators of cytokine-activated Janus kinase/signal transducers and activators of transcription signaling pathway, but increasing evidence reveals that SOCS proteins play an important role in the development of type 2 diabetes...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-014-3079-8
更新日期：2014-01-01 00:00:00
abstract：:Transition from laying to incubation behavior in chicken is an interesting topic in reproductive biology. The decline of incubation behavior in chicken population has led to considerable phenotypic differences in reproductive traits between breeds. However, the exact genetic mechanism of the reproductive phase transit...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-4033-8
更新日期：2016-09-01 00:00:00
abstract：:Oryza rufipogon L. (O. rufipogon) or a common wild rice, showed considerable aluminum (Al) tolerance. In this study, we examined the physiologic and genetic response of wild rice short term and long term to Al toxicity, respectively. In the short term study, morin staining, DAPI staining and aniline blue staining were...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-010-0618-9
更新日期：2011-11-01 00:00:00
abstract：:RACK1 is a crucial scaffold and anchoring protein, which plays a vital role in multiple signaling pathways of tumorigenesis. The aim of the present study was to identify the correlation between expressions of RACK1 and malignant degrees in colorectal carcinoma (CRC) patients. All together 157 CRC patients were enrolle...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3201-y
更新日期：2014-05-01 00:00:00
abstract：:Several lines of evidence suggest that the ubiquitin-proteasome pathway is involved in sepsis-induced muscle catabolism. The gene expression of ubiquitin and several of the proteasome subunits was increased in muscle from both septic rats and patients. In other studies, the activity of isolated 20S proteasomes was sti...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1023/a:1006916206260
更新日期：1999-04-01 00:00:00
