Abstract:
:Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Amesz H,Haubrich T,Voorma HOdoi
10.1007/BF00777498subject
Has Abstractpub_date
1979-05-31 00:00:00pages
121-5issue
1-2eissn
0301-4851issn
1573-4978journal_volume
5pub_type
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更新日期:2011-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11033-006-0005-8
更新日期:2006-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11033-018-4329-y
更新日期:2018-12-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
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更新日期:2018-12-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1023/a:1006826725056
更新日期:1997-03-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0367-9
更新日期:2011-04-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-008-9319-z
更新日期:2009-07-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3537-3
更新日期:2014-10-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4236-2
更新日期:2018-10-01 00:00:00
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journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0332-7
更新日期:2011-03-01 00:00:00