Abstract：
:The Giant Panda is an endangered and valuable gene pool in genetic, its important functional gene POLR2H encodes an essential shared peptide H of RNA polymerases. The genomic DNA and cDNA sequences were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) adopting touchdown-PCR and reverse transcription polymerase chain reaction (RT-PCR), respectively. The length of the genomic sequence of the Giant Panda is 3,285 bp, including five exons and four introns. The cDNA fragment cloned is 509 bp in length, containing an open reading frame of 453 bp encoding 150 amino acids. Alignment analysis indicated that both the cDNA and its deduced amino acid sequence were highly conserved. Protein structure prediction showed that there was one protein kinase C phosphorylation site, four casein kinase II phosphorylation sites and one amidation site in the POLR2H protein, further shaping advanced protein structure. The cDNA cloned was expressed in Escherichia coli, which indicated that POLR2H fusion with the N-terminally His-tagged form brought about the accumulation of an expected 20.5 kDa polypeptide in line with the predicted protein. On the basis of what has already been achieved in this study, further deep-in research will be conducted, which has great value in theory and practical significance.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Du YJ,Hou YL,Hou WRdoi
10.1007/s11033-012-2192-9subject
Has Abstractpub_date
2013-02-01 00:00:00pages
1495-8issue
2eissn
0301-4851issn
1573-4978journal_volume
40pub_type
杂志文章abstract：:Transition from laying to incubation behavior in chicken is an interesting topic in reproductive biology. The decline of incubation behavior in chicken population has led to considerable phenotypic differences in reproductive traits between breeds. However, the exact genetic mechanism of the reproductive phase transit...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-016-4033-8
更新日期：2016-09-01 00:00:00
abstract：:PNAS-4 is a novel pro-apoptotic protein activated during the early response to DNA damage; however, the molecular mechanisms and pathways regulating PNAS-4 expression in tumors are not well understood. We hypothesized that PNAS-4 is a p53 down-stream target gene and designed this study. We searched online for putative...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0732-3
更新日期：2012-01-01 00:00:00
abstract：:Late blight caused by Phytophthora infestans is the most important disease of potato. Many efforts have been made to understand molecular mechanism of the durable resistance to address the challenge raised by rapid evolution of the pathogen. A pathogenesis related protein (PR) gene StPRp27 was previously isolated from...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0937-5
更新日期：2012-02-01 00:00:00
abstract：:Phage display procedure was applied to the N-terminal domain of human topoisomerase I. The consensus sequence identified for clones binding to the N-terminal domain was found in 35 human proteins that are either permanently or temporarily located in the nucleus. They are in majority involved in the DNA repair, transcr...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1023/a:1021237021338
更新日期：2002-12-01 00:00:00
abstract：:Studies investigating the association between glutathione S-transferase M1 (GSTM1) polymorphism and bladder cancer risk report conflicting results. The objective of this study was to quantitatively summarize the evidence for such a relationship. We performed a systematic search of the National Library of Medline and E...
journal_title：Molecular biology reports
pub_type： 杂志文章,meta分析
doi：10.1007/s11033-010-0386-6
更新日期：2011-04-01 00:00:00
abstract：:CyclinD1 over-expression is the key pathogenetic event underlying airway smooth muscle (ASM) proliferation. Human antigen R (HuR) is a ubiquitously expressed RNA-binding protein, and is known to regulate the expression of multiple cell cycle regulators. The aim of the study is to investigate whether HuR might also be ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1392-z
更新日期：2012-05-01 00:00:00
abstract：:Microsatellites, or simple sequence repeats (SSRs), are very useful molecular markers for a number of plant species. We used a new publicly available module (TROLL) to extract microsatellites from the public database of soybean expressed sequence tag (EST) sequences. A total of 12,833 sequences containing di- to penta...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1776-8
更新日期：2012-09-01 00:00:00
abstract：:Maize (Zea mays L.) is an important model plant with an important role in agriculture and national economies all over the world. The optimum growth temperature of maize is between 25 and 28 °C. At temperatures below 12 °C, maize is vulnerable to damage by chilling stress. MYB transcription factors play important roles...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-04840-5
更新日期：2019-08-01 00:00:00
abstract：:Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-014-3633-4
更新日期：2014-11-01 00:00:00
abstract：:Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in se...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1591-2
更新日期：2012-07-01 00:00:00
abstract：:The present study brings out the preventive role of (-)-epigallocatechin-gallate (EGCG) on cardiac mitochondrial metabolism and apoptosis in cigarette smoke (CS)-exposed rats. The CS-exposed rats showed significantly decreased activities of TCA cycle enzymes and mitochondrial enzymatic antioxidants, on the other hand,...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2673-5
更新日期：2013-12-01 00:00:00
abstract：:The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-019-05083-0
更新日期：2019-12-01 00:00:00
abstract：:We have previously shown that angiotensin-converting enzyme (ACE) gene D allele is an independent risk factor for early onset coronary artery disease (CAD). Little is known about the concomitant presence of the ACE gene D allele and paraoxonase (PON1) codon 192 arginine (Arg) on the severity of CAD. Regarding the high...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1027-4
更新日期：2012-03-01 00:00:00
abstract：:Local domestic chicken populations are of paramount importance as a source of protein in developing countries. Bangladesh possesses a large number of native chicken populations which display a broad range of phenotypes well adapted to the extreme wet and hot environments of this region. This and the fact that wild jun...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2522-6
更新日期：2013-06-01 00:00:00
abstract：:Tomato bacterial wilt (BW) incited by Ralstonia solanacearum is a constraint on tomato production in tropical, subtropical and humid regions of the world. In this paper, we present the results of a research aimed at the identification of PCR-based markers amplified fragment length polymorphism (AFLP) linked to the gen...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9204-1
更新日期：2009-03-01 00:00:00
abstract：:Testis development is a highly regulated sequence of developmental process that spans from the establishment of germ cell lineage during embryonic development to the periodic wave of spermatogenesis in adulthood. The normal development of testes and the fertility of male animals require specific cell types to respond ...
journal_title：Molecular biology reports
pub_type： 杂志文章,评审
doi：10.1007/s11033-011-1265-5
更新日期：2012-04-01 00:00:00
abstract：:Toll-like receptors (TLRs) constitute a multi-gene family, which plays a pivotal role in sensing invading pathogens by virtue of conserved microbial patterns. TLR repertoire of chicken and zebra finch has been well studied. However TLR family of other avian species is yet to be characterized. In the present study, we ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1709-6
更新日期：2012-08-01 00:00:00
abstract：:The aim of this work was to sequence the promoter region of β-lactoglobulin (BLG) gene in four sheep breeds, in order to identify polymorphisms, infer and analyze haplotypes, and phylogenetic relationship among the Valle del Belice breed and the other three breeds considered as ancestors. Sequencing analysis and align...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-0794-2
更新日期：2012-01-01 00:00:00
abstract：:Intrinsic bent DNA sites were identified in the 4289 bp segment encompassing the replication zone which directs DNA amplification and transcription of the C3-22 gene of Rhynchosciara americana. Restriction fragments showed reduced electrophoretic mobility in polyacrylamide gels. The 2D modeling of the 3D DNA path and ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-006-0009-4
更新日期：2006-03-01 00:00:00
abstract：:We reported that an aqueous extract of Portulaca oleracea L. inhibited high-fat-diet-induced oxidative injury in a dose-dependent manner. Male kunming mice (5-weeks-old, 24 g) were used in this experiment. After a 4-day adaptation period, animals were randomly divided into four groups (n = 10 in each group); Group 1: ...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1644-6
更新日期：2012-08-01 00:00:00
abstract：:Transcriptome from high throughput sequencing-by-synthesis is a good resource of molecular markers. In this study, we present utility of massively parallel sequencing by synthesis for profiling the transcriptome of red pepper (Capsicum annuum L. TF68) using 454 GS-FLX pyrosequencing. Through the generation of approxim...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-011-1102-x
更新日期：2012-03-01 00:00:00
abstract：:In order to extend the knowledge of rabies pathogenesis, a two-dimensional electrophoresis/mass spectrometry based postmortem comparative proteomics analysis was carried out on human brain samples. Alteration in expression profile of several proteins was detected. Proteins related to cytoskeleton, metabolism, proteaso...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-013-2759-0
更新日期：2013-11-01 00:00:00
abstract：:Small ubiquitin-like modifier (SUMO) participates in post-translational modification of various target proteins. SUMOylation is an important molecular regulatory mechanism for plants to respond to abiotic stress. In the present study, GmSUMO2 gene was isolated from soybean seedlings for further study because of the hi...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05433-3
更新日期：2020-05-01 00:00:00
abstract：:Porcine MYOD1 gene has been mapped to swine chromosome (SSC) 2p14-p17, which is involved in the regulation of the proliferation and differentiation of skeletal muscle cells. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1) genes, which map close to MYOD1, are involved in energy m...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9882-y
更新日期：2010-01-01 00:00:00
abstract：:Deprivation of cytokines induces cell cycle arrest and apoptosis in cytokine-dependent hematopoietic progenitors. Previous studies have indicated that in Baf-3, interleukin (IL)-3-dependent cells, apoptosis is caused predominantly by Bim, a BH3-only cell death activator that belongs to the Bcl-2 superfamily. Because B...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-004-7656-0
更新日期：2005-06-01 00:00:00
abstract：:In clinic, the patients with acute myocardial infarction (AMI) are at high risk to develop ischemia-induced ventricular arrhythmias leading to sudden cardiac death (SCD). Some studies suggest that individual susceptibility to ischemia-induced arrhythmia may be related to the genes encoding ion channels. One of them is...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-007-9060-z
更新日期：2008-06-01 00:00:00
abstract：:Ischemic postconditioning has been described in both heart and brain. The first aim of this study was to evaluate the effects of Sevoflurane postconditioning (SP) on brain biochemical parameters, Bcl-2, Bax, c-Fos and Caspase-3 protein levels and Bcl-2, Bax, TNF-α and Caspase-3 mRNA expression in the middle cerebral a...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1935-y
更新日期：2012-12-01 00:00:00
abstract：:Telomerase activity is repressed in normal human somatic cells, but is activated in most cancers, suggesting that telomerase may be an important target for cancer therapy. Agents that interact selectively with telomerase are anticipated to exert specific action on cancer cells. In this study, we evaluated maleimide de...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-012-1757-y
更新日期：2012-09-01 00:00:00
abstract：:Regulation of the sumoylation system at the level of gene expression has not yet been explored. To begin to define transcriptional regulatory features, the promoter region for the SUMO1 gene was cloned from human genomic DNA and characterized. Initially, a 532 base pair fragment upstream of and including the predicted...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-009-9476-8
更新日期：2010-03-01 00:00:00
abstract：:Rice production in recent years is highly affected by rapidly increasing temperatures in the tropical and sub-tropical countries, which threatens the sustainable production in near future. Hence, understanding the heat tolerance mechanism and evolving tolerant varieties is an immense need in the staple crop rice. An e...
journal_title：Molecular biology reports
pub_type： 杂志文章
doi：10.1007/s11033-020-05291-z
更新日期：2020-03-01 00:00:00
