当前位置： SCI文献检索 > MOLECULAR BIOLOGY REPORTS期刊下所有文献 > Identification of novel homologues of three low molecular weight subunits of the mitochondrial bc1 complex.

Identification of novel homologues of three low molecular weight subunits of the mitochondrial bc1 complex.

Abstract：

:Large-scale random cDNA sequencing projects have been started for several organisms and are a valuable tool for the analysis of quantitative and qualitative aspects of gene expression. However, the reliability of the obtained data is limited as most of the clones are only partially analysed on one strand. As a consequence the sequence entries derived from random cDNA sequencing projects usually comprise incomplete open reading frames. They nevertheless define complete and reliable coding sequences, if two prerequisites are fulfilled: (i) the clones encode very small proteins, and (ii) the clones have a high frequency in the cDNA-banks. The present study describes the use of cDNA databases for the identification of homologues of three low-molecular-weight subunits of the mitochondrial bc1 complex, termed the QCR6, QCR9 and QCR10 proteins. These polypeptides are only characterized for a small number of organisms, have a scarcely defined function and exhibit a low degree of structural conservation if compared between different species. Several clones were identified for each polypeptide by searches with TBLASTN using the known sequences as probes. Most of the database entries contain complete open reading frames and sequencing queries could be excluded due to the abundancy of the clones. Multiple sequence alignments are presented for all three polypeptides and consensus sequences are given which may provide a basis for the investigation of the proteins by site-directed mutagenesis.

journal_name

Mol Biol Rep

journal_title

Molecular biology reports

authors

Braun HP

doi

10.1007/BF00424432

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

71-7

issue

2

eissn

0301-4851

issn

1573-4978

journal_volume

23

pub_type

杂志文章

相关文献

MOLECULAR BIOLOGY REPORTS文献大全
  • Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews.

    abstract：:Apolipoprotein AV (apoAV) modulates plasma triglyceride levels, which is an independent risk factor for cardiovascular disease. ApoAV is also involved in atherosclerosis lesion formation. In order to systematically evaluate the apolipoprotein-related gene profile in tree shrew, a model for its insusceptibility to athe...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-013-2641-0

    authors： Li G,Luo H,Sun G,Wu G,Wu G,Wang Y,Man Y,Wang S,Li J,Chen B

    更新日期：2013-09-01 00:00:00

  • Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.

    abstract：:Recent reports on various cancer models demonstrate a great potential of cytosine deaminase/5-fluorocytosine suicide system in cancer therapy. However, this approach has limited success and its application to patients has not reached the desirable clinical significance. Accordingly, the improvement of this suicide sys...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-017-4113-4

    authors： Komissarov A,Demidyuk I,Safina D,Roschina M,Shubin A,Lunina N,Karaseva M,Kostrov S

    更新日期：2017-08-01 00:00:00

  • Haplotypes of CYP1B1 and CCDC57 genes in an Afro-Caribbean female population with uterine leiomyoma.

    abstract：:Uterine leiomyomas (UL) are prevalent benign tumors, especially among women of African ancestry. The disease also has genetic liability and is influenced by risk factors such as hormones and obesity. This study investigates the haplotypes of the Cytochrome P450 1B1 gene (CYP1B1) related to hormones and coiled-coil dom...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-019-04790-y

    authors： Alleyne AT,Bideau VS

    更新日期：2019-06-01 00:00:00

  • Cortactin and Exo70 mediated invasion of hepatoma carcinoma cells by MMP-9 secretion.

    abstract：:This study was aimed to evaluate the regulation mechanism of cortactin (CTTN) on matrix metalloproteinases 9 (MMP-9) and its relations with Exo70 in invasion of hepatoma carcinoma (HCC) cells. The expression levels of CTTN, Exo70 and MMP-9 were detected in normal hepatocytes and various HCC cells by real-time PCR. The...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-016-3972-4

    authors： Zhao G,Zhang H,Huang Z,Lv L,Yan F

    更新日期：2016-05-01 00:00:00

  • Copy number variations of chromosome 17p13.1 might be linked to high risk of lung cancer in heavy smokers.

    abstract：:Lung cancer is the most common cause of cancer death worldwide. Smoking is known as the strongest single factor in the development of lung cancer. However, there are inherited genetic factors that cause different responses to cigarette smoking exposure among individuals. We tried to identify these differences in heavy...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0672-3

    authors： Lee M,Lee Y,Cho HJ,Hong J,Kwon SJ,Park CG,Lee HY,Son JW,Kang J

    更新日期：2011-11-01 00:00:00

  • An association between polymorphism of the heme oxygenase-1 and -2 genes and age-related macular degeneration.

    abstract：:Iron may be implicated in the generation of oxidative stress by the catalyzing the Haber-Weiss or Fenton reaction. On the other hand, oxidative stress has been implicated in the pathogenesis of age-related macular degeneration (AMD) and heme oxygenase-1 (HO-1), encoded by the HMOX1 gene and heme oxygenase-2 (HO-2), en...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0955-3

    authors： Synowiec E,Szaflik J,Chmielewska M,Wozniak K,Sklodowska A,Waszczyk M,Dorecka M,Blasiak J,Szaflik JP

    更新日期：2012-03-01 00:00:00

  • Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina.

    abstract：:In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via t...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0840-0

    authors： Meng Q,Xiao Z,Yuan H,Xue F,Zhu Y,Zhou X,Yang B,Sun J,Meng B,Sun X,Cheng F

    更新日期：2012-02-01 00:00:00

  • Serum levels of testosterone and gonadotrophins with respect to smoking status and genetic polymorphism of GSTT1.

    abstract：OBJECTIVES:It is reported that parental exposure to toxicants can influence offspring sex ratio at birth. Studies have reported that several chemicals found in cigarette smoke are substrates of glutathione S-transferase T1 (GSTT1, a member of GSTtheta). To determine the effect of cigarette smoke on serum levels of test...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-008-9319-z

    authors： Saadat M

    更新日期：2009-07-01 00:00:00

  • An integrated pathway system modeling of Saccharomyces cerevisiae HOG pathway: a Petri net based approach.

    abstract：:Biochemical networks comprise many diverse components and interactions between them. It has intracellular signaling, metabolic and gene regulatory pathways which are highly integrated and whose responses are elicited by extracellular actions. Previous modeling techniques mostly consider each pathway independently with...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-2153-3

    authors： Tomar N,Choudhury O,Chakrabarty A,De RK

    更新日期：2013-02-01 00:00:00

  • In silico analysis of the cyanobacterial lectin scytovirin: new insights into binding properties.

    abstract：:Scytovirin is a lectin isolated from the cyanobacterium Scytonema varium that has shown activity against HIV, SARS coronavirus and Zaire Ebola virus. Its 95 amino acids are divided into two structural domains (SD), the first spanning amino acids 1-48 (SD1) and the second 49-95 (SD2). Interestingly, the domains are nea...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-017-4116-1

    authors： Siqueira AS,Lima ARJ,de Souza RC,Santos AS,Vianez Júnior JLDSG,Gonçalves EC

    更新日期：2017-08-01 00:00:00

  • Unloading the infarcted heart affect MMPs-TIMPs axis in a rat cardiac heterotopic transplantation model.

    abstract：:Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induc...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-0736-z

    authors： Wang WJ,Meng ZL,Mo YC,Liu JW,Sun CC,Hu SS,Zhang H

    更新日期：2012-01-01 00:00:00

  • Kinetic and thermodynamic study of cloned thermostable endo-1,4-β-xylanase from Thermotoga petrophila in mesophilic host.

    abstract：:The 1,044 bp endo-1,4-β-xylanase gene of a hyperthermophilic Eubacterium, "Thermotoga petrophila RKU 1" (T. petrophila) was amplified, from the genomic DNA of donor bacterium, cloned and expressed in mesophilic host E. coli strain BL21 Codon plus. The extracellular target protein was purified by heat treatment followe...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1555-6

    authors： ul Haq I,Hussain Z,Khan MA,Muneer B,Afzal S,Majeed S,Akram F

    更新日期：2012-07-01 00:00:00

  • MicroRNA signature associated with osteogenic lineage commitment.

    abstract：:Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in se...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-1591-2

    authors： Bakhshandeh B,Soleimani M,Hafizi M,Paylakhi SH,Ghaemi N

    更新日期：2012-07-01 00:00:00

  • In vitro interaction between the N-terminus of the Ewing's sarcoma protein and the subunit of RNA polymerase II hsRPB7.

    abstract：:In vivo and in vitro expressed N-terminal sequence of EWS (EAD) and hsRPB7 (subunit of human RNA polymerase II) were probed for protein-protein interactions using pull-down assays. In result, it was found that the proteins 57Z (residues 1-57 of EAD) and hsRPB7 interact in vitro forming a stable complex. The direct int...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-008-9308-2

    authors： Todorova R

    更新日期：2009-07-01 00:00:00

  • Genome-wide survey and expression analysis of the MADS-box gene family in soybean.

    abstract：:MADS-box genes encode important transcription factors in plants that are involved in many processes during plant growth and development. An investigation of the soybean genome revealed 106 putative MADS-box genes. These genes were classified into two classes, type I and type II, based on phylogenetic analysis. The soy...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-012-2438-6

    authors： Shu Y,Yu D,Wang D,Guo D,Guo C

    更新日期：2013-06-01 00:00:00

  • IiSDD1, a gene responsive to autopolyploidy and environmental factors in Isatis indigotica.

    abstract：:In plants, stomata play a pivotal role in the regulation of gas exchange and are distributed throughout the aerial epidermis. SDD1, a gene isolated from Arabidopsis thaliana has been demonstrated to specialize in stomatal density and distribution. In our present study, a comprehensive survey of global gene expression ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-009-9776-z

    authors： Xiao Y,Yu X,Chen J,Di P,Chen W,Zhang L

    更新日期：2010-02-01 00:00:00

  • A shift in the hepatitis C virus genotype dominance in blood donor samples from Thailand.

    abstract：:Hepatitis C virus (HCV) can be classified into six major genotypes. The HCV genotypes variability accounts for its geographical distribution, its responses to treatments and the clinical outcomes. The aim of this study was to determine the distribution of HCV genotypes among volunteer blood donors in Thailand. Samples...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0552-x

    authors： Sistayanarain A,Kunthalert D,Vipsoongnern Y

    更新日期：2011-10-01 00:00:00

  • Studies on protein organization of nucleosomes using cross-linking.

    abstract：:When chromosomal proteins in chromatin or in mononucleosomes were extensively cross-linked with an imido ester, the H1-containing nonameric histone complex was revealed. In this complex, histone H1 is connected with the octamer of core histones. The cross-linking of H1 of the octamer is realized preferentially through...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00805754

    authors： Bakayev VV,Bakayeva TG,Domansky NN

    更新日期：1981-08-14 00:00:00

  • Investigation of the interaction between CREB-binding protein and STAT4/STAT6.

    abstract：:Coactivator CBP (CREB-binding protein) has been implicated in the regulation of transcription for all signal transducer and activator of transcription factors (STATs); however, the mechanism remains unclear. Using yeast two-hybrid screening and immunoprecipitation techniques, we investigated the direct interaction of ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0622-0

    authors： Zhang M,Fu Z,Tian D,Liu E,Dai J,Wang L

    更新日期：2011-10-01 00:00:00

  • Cross-priming of microsatellite loci in subfamily cyprininae (family Cyprinidae): their utility in finding markers for population genetic analysis in three Indian major carps.

    abstract：:This study is aimed to identify polymorphic microsatellite markers and establish their potential for population genetics studies in three carp (family cyprinidae; subfamily cyprininae) species, Labeo rohita, Catla catla and Cirrhinus mrigala through use of cyprinid primers. These species have high commercial value and...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3386-0

    authors： Masih P,Luhariya RK,Das R,Gupta A,Mohindra V,Singh RK,Srivastava R,Chauhan UK,Jena JK,Lal KK

    更新日期：2014-08-01 00:00:00

  • Three mRNA populations differing in turnover and processing in mouse liver.

    abstract：:Using kinetics of approach to steady state labeling, we have found that liver poly(A)+ mRNAs fall into three populations, differing in stability and probably in processing, as reflected in their dissimilar delays in reaching polyribosomes and turnover times. There are mRNA-1 (delay 10 min, half-life 1 hr); mRNA-2(dela...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00357013

    authors： Lubimova EV,Chernovskaja TV,Lerman MI

    更新日期：1975-12-01 00:00:00

  • A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows.

    abstract：:PCR low ionic strength single-strand conformation polymorphism (PCR-LIS-SSCP) and DNA sequencing methods were used to analyze the polymorphisms within the coding region of bovine ATP1A1 gene. A novel C/A mutation was identified at the nucleotide position 2789 of the ATP1A1 mRNA, but it was silent with respect to the a...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0080-8

    authors： Liu Y,Li D,Li H,Zhou X,Wang G

    更新日期：2011-01-01 00:00:00

  • Genetic diversity in some grape varieties revealed by SCoT analyses.

    abstract：:Start codon targeted (SCoT) polymorphic markers were used to assess genetic relationships among 64 grape varieties. Seventeen informative primers were selected from 36 SCoT primers based on their ability to produce clear and repeatable polymorphic and unambiguous bands among the varieties. A total of 131 bands were pr...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-011-1329-6

    authors： Guo DL,Zhang JY,Liu CH

    更新日期：2012-05-01 00:00:00

  • DNA sequence specific interactions of histone H1.

    abstract：:Histone H1 is know to play a role in the formation and maintenance of higher-order chromatin structure. It has been recently suggested that the linker histone might be also involved in the regulation of the activity of individual genes. If H1 is a regulatory factor in eukaryotic gene transcription, it should possess s...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,评审

    doi：10.1007/BF00369901

    authors： Zlatanova J,Yaneva J

    更新日期：1991-02-01 00:00:00

  • Tobacco microRNAs prediction and their expression infected with Cucumber mosaic virus and Potato virus X.

    abstract：:MicroRNAs (miRNAs) are a newly identified class of non-coding small RNAs of about 21-24 nucleotides. They play important roles in multiple biological processes by degrading targeted mRNAs or repressing mRNA translation. To date, a total of 2,043 plant miRNAs are present in the miRNA Registry database (miRBase Release ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0260-6

    authors： Lang Q,Jin C,Lai L,Feng J,Chen S,Chen J

    更新日期：2011-03-01 00:00:00

  • Effect of BRAF V600E mutation on tumor response of anti-EGFR monoclonal antibodies for first-line metastatic colorectal cancer treatment: a meta-analysis of randomized studies.

    abstract：:Anti-EGFR monoclonal antibodies (anti-EGFR MoAbs) in metastatic colorectal cancer (mCRC) treatment are still not effective in all patients. This study aimed to evaluate the relationship between BRAF V600E mutation and the tumor response of anti-EGFR MoAbs for first-line treatment in mCRC patients. We searched the MEDL...

    journal_title：Molecular biology reports

    pub_type： 杂志文章,meta分析

    doi：10.1007/s11033-013-2974-8

    authors： Cui D,Cao D,Yang Y,Qiu M,Huang Y,Yi C

    更新日期：2014-03-01 00:00:00

  • Functional characterization of a Δ5-like fatty acyl desaturase and its expression during early embryogenesis in the noble scallop Chlamys nobilis Reeve.

    abstract：:Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential in lots of important physiological processes, while, many marine species have no or limited ability of endogenous PUFA biosynthesis, normally due to the lack of key enzymes such as fatty acid desaturase (FAD). In this study, we isolated a scallop Chlamys ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-014-3633-4

    authors： Liu H,Guo Z,Zheng H,Wang S,Wang Y,Liu W,Zhang G

    更新日期：2014-11-01 00:00:00

  • The dual identities of mammalian tRNA(Sec) for SerRS and selenocysteine synthase.

    abstract：:Se is an essential trace element and is found as a selenocysteine in the active site of Se-enzymes, such as glutathione peroxidase. tRNASec is first aminoacylated with serine by Ser RS and further is converted to selenocysteyl-tRNA by selenocysteine synthase. Mammalian selenocysteine tRNA has dual identities with Ser ...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1023/a:1006879820805

    authors： Mizutani T,Kanaya K,Ikeda S,Fujiwara T,Yamada K,Totsuka T

    更新日期：1998-11-01 00:00:00

  • A few distinct 'molecular sandwiches' are basis for structural and functional similarities of subspecies of interferon alpha and of families of growth-promoting hormones.

    abstract：:'Molecular sandwiches' composed of two aromatic amino acids separated by a hydrophilic one were found on eleven subspecies of human interferon alpha, on murine interferon alpha 2, and human interferon beta 1. In addition, another type of the sandwiches was found on several species of interferon. This confirms and exte...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/BF00417593

    authors： Inglot AD,Popik W,Piasecki E,Czyrski J

    更新日期：1986-01-01 00:00:00

  • Phylogenetic analysis of 16S rRNA gene sequences reveals distal gut bacterial diversity in wild wolves (Canis lupus).

    abstract：:The aim of this study was to describe the microbial communities in the distal gut of wild wolves (Canis lupus). Fecal samples were collected from three healthy unrelated adult wolves captured at the nearby of Dalai Lake Nature Reserve in Inner Mongolia of China. The diversity of fecal bacteria was investigated by cons...

    journal_title：Molecular biology reports

    pub_type： 杂志文章

    doi：10.1007/s11033-010-0060-z

    authors： Zhang H,Chen L

    更新日期：2010-12-01 00:00:00