Abstract:
:Multiple cellular proteins have been identified as participating in Toll/interleukin-1 receptor-mediated inflammatory gene expression. The continuing isolation of novel components, based on sequence similarities, protein-protein interactions and protein purification, suggests that many elements of this signalling network remain to be identified. We report here the development of a high-throughput functional screening platform and its application for the identification of components of inflammatory signalling networks. Our results enable us to estimate that 100-150 gene products are involved in controlling the transcription of the human interleukin 8 gene. The approach, which is simple and robust, constitutes a general method for mapping signal transduction systems and for rapid isolation of a large number of signalling components based on the control of pathways leading to regulation of gene expression.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Kiss-Toth E,Wyllie DH,Holland K,Marsden L,Jozsa V,Oxley KM,Polgar T,Qwarnstrom EE,Dower SKdoi
10.1042/BST20051405keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
1405-6issue
Pt 6eissn
0300-5127issn
1470-8752pii
BST20051405journal_volume
33pub_type
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