Abstract:
:Non-coding transcripts play an important role in gene expression regulation in all species, including budding and fission yeast. Such regulatory transcripts include intergenic ncRNA (non-coding RNA), 5' and 3' UTRs, introns and antisense transcripts. In the present review, we discuss advantages and limitations of recently developed sequencing techniques, such as ESTs, DNA microarrays, RNA-Seq (RNA sequencing), DRS (direct RNA sequencing) and TIF-Seq (transcript isoform sequencing). We provide an overview of methods applied in yeast and how each of them has contributed to our knowledge of gene expression regulation and transcription.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Schlackow M,Gullerova Mdoi
10.1042/BST20130144subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1654-9issue
6eissn
0300-5127issn
1470-8752pii
BST20130144journal_volume
41pub_type
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