Abstract:
:Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and alpha-tricalcium phosphate (alpha-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-alpha-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial; mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-alpha-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-alpha-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-alpha-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-alpha-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.
journal_name
Dent Mater Jjournal_title
Dental materials journalauthors
Yuasa T,Miyamoto Y,Kon M,Ishikawa K,Takechi M,Momota Y,Tatehara S,Takano H,Mimamiguchi S,Nagayama Mdoi
10.4012/dmj.24.207keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
207-12issue
2eissn
0287-4547issn
1881-1361journal_volume
24pub_type
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