Abstract：
:COPII vesicles assemble at ER subdomains called transitional ER (tER) sites, but the mechanism that generates tER sites is unknown. To study tER biogenesis, we analyzed the transmembrane protein Sec12, which initiates COPII vesicle formation. Sec12 is concentrated at discrete tER sites in the budding yeast Pichia pastoris. We find that P. pastoris Sec12 exchanges rapidly between tER sites and the general ER. The tER localization of Sec12 is saturable and is mediated by interaction of the Sec12 cytosolic domain with a partner component. This interaction apparently requires oligomerization of the Sec12 lumenal domain. Redistribution of P. pastoris Sec12 to the general ER does not perturb the localization of downstream tER components, suggesting that Sec12 and other COPII proteins associate with a tER scaffold. These results provide evidence that tER sites form by a network of dynamic associations at the cytosolic face of the ER.
journal_name
Dev Celljournal_title
Developmental cellauthors
Soderholm J,Bhattacharyya D,Strongin D,Markovitz V,Connerly PL,Reinke CA,Glick BSdoi
10.1016/s1534-5807(04)00129-7keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
649-59issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(04)00129-7journal_volume
6pub_type
杂志文章abstract：:In the last ten years, we have made considerable progress in our genetic and molecular understanding of all aspects of skeletal development, chondrogenesis, joint formation, and osteogenesis. This review addresses the role of the principal growth factors and transcription factors affecting these different processes an...
journal_title：Developmental cell
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abstract：:Positive autoregulation is an effective mechanism for the long-term maintenance of a transcription factor's expression. This strategy is widely deployed in cell lineages, where the autoregulatory factor controls the activity of a battery of genes that constitute the differentiation program of a postmitotic cell type. ...
journal_title：Developmental cell
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abstract：:Transmission of the human parasite Brugia malayi relies on the sustained production of larvae in blood. In this issue of Developmental Cell,Foray et al. (2018) use methods developed in the model nematode C. elegans to reveal how a symbiotic bacterium supports the female germ cell development underlying this massive fe...
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pub_type： 评论,杂志文章
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abstract：:The large GTPase Dynamin 2 (Dyn2) is markedly upregulated in pancreatic cancer, is a potent activator of metastatic migration, and is required for Rac1-mediated formation of lamellipodia. Here we demonstrate an unexpected mechanism of Dyn2 action in these contexts via direct binding to the Rac1 guanine nucleotide exch...
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journal_title：Developmental cell
pub_type： 杂志文章,评审
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journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 杂志文章
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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