Abstract：
:A way to convert the volume change of a biochemo-mechanical gel into the change in liquid column length was developed. Our trial sensor device consisted of a small compartment for incorporating the gel, a flow channel with a filled dye solution, and a poly(dimethylsiloxane) (PDMS) diaphragm by which the gel and the dye solution were separated. A lightly cross-linked N-isopropylacrylamide (NIPAAm)/acrylic acid (AA) copolymer gel with immobilized glucose oxidase was used as a sensing element. It was found that a change in the gel volume caused by the immobilized enzyme reaction was accurately converted into a change of the column length (Deltal) with the help of the PDMS diaphragm. By use of a cylindrical gel (diameter approximately 2 and thickness approximately 1 mm), the time curve of Deltal varied depending upon glucose concentration over a range of 0.2-50 mM; in particular, it is of importance that semilogarithmic plots of Deltal (in mm) against glucose concentration (in mM) can be used as a calibration curve. For glucose solutions of mM order, 1 min was enough to determine the concentrations, whereas 10 min was required for concentrations of microM order. When the measurement time was limited within 10 min, the lower detection limit was 200 microM. The response was affected by buffering capacity of the samples, but this was controllable through reduction of the sample volume. These results indicate that the present way can be used for the determination of glucose concentration.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Suzuki H,Kumagai A,Ogawa K,Kokufuta Edoi
10.1021/bm034331qkeywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
486-91issue
2eissn
1525-7797issn
1526-4602journal_volume
5pub_type
杂志文章abstract：:A novel approach for the preparation of nano- and microcapsules in aqueous solutions by using thermoresponsive polymer (TRP) templates (mesoglobules) is described. The method comprised three steps: formation of mesoglobules, coating the templates by seeded radical copolymerization, followed by core dissolution and cor...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm501194g
更新日期：2014-12-08 00:00:00
abstract：:Spider dragline silk with its superlative tensile properties provides an ideal system to study the relationship between morphology and mechanical properties of a structural protein. Accordingly, we synthesized two hybrid multiblock copolymers by condensing poly(alanine) [(Ala)(5)] blocks of the structural proteins (sp...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060199t
更新日期：2006-08-01 00:00:00
abstract：:A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell line...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00776
更新日期：2016-10-10 00:00:00
abstract：:Tissue engineering approaches for peripheral nerve regeneration employ biodegradable scaffolds coupled with growth factors for improved performance in regeneration of large nerve injuries. Electrospun nanofibers provide a versatile platform for fabrication of scaffolds with extracellular matrix like architecture and i...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8012499
更新日期：2009-05-11 00:00:00
abstract：:Biodegradable aliphatic polyesters such as poly(lactide) and poly(ε-caprolactone), largely used in tissue engineering applications, lack suitable functional groups and biological cues to enable interactions with cells. Because of the ubiquity of thiol groups in the biological environment and the pliability of thiol ch...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00005
更新日期：2016-04-11 00:00:00
abstract：:The development of improved cryopreservative materials is necessary to enable complete recovery of living cells and tissue after frozen storage. Remarkably, poly(vinyl alcohol) (PVA) displays some of the same cryoprotective properties as many antifreeze proteins found in cold tolerant organisms. In particular, PVA is ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01502
更新日期：2018-01-08 00:00:00
abstract：:Silkworm silk has excellent mechanical properties, biocompatibility, and promising applications in the biomedical sector. Silkworms spin silk at the beginning and end of each of their five instar stages, as well as spinning mature silk after the fifth instar. We evaluated the mechanical properties and structure of 10 ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01576
更新日期：2019-03-11 00:00:00
abstract：:A scanning laser system has been used to generate three-dimensional trimethylolpropane trimethacrylate (TRIM) cross-linked poly(2-hydroxylethyl methacrylate) polymer microstructures through azo-bis(isobutyro)nitrile (AIBN) photopolymerization using a 20 x 0.5 NA microscope objective and 365 nm laser excitation. Macrop...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0506952
更新日期：2006-03-01 00:00:00
abstract：:Thymic epithelial cells (TECs) are the main regulators of T lymphocyte development and selection, requiring a three-dimensional (3D) environment to properly perform these biological functions. The aim of this work was to develop a 3D culture substrate that allows the survival and proliferation of TECs. Thus, electrosp...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00933
更新日期：2020-12-14 00:00:00
abstract：:An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201143c
更新日期：2012-01-09 00:00:00
abstract：:A unified kinetic pathway for the enzyme-catalyzed polymerization and degradation of poly(ε-caprolactone) was developed. This model tracks the complete distribution of individual chain lengths, both enzyme-bound and in solution, and successfully predicts monomer conversion and the molecular mass distribution as a func...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2009312
更新日期：2011-09-12 00:00:00
abstract：:External magnetic field is a powerful approach to induce orientational order in originally disordered suspensions of magneto-responsive anisotropic particles. By small angle neutron scattering and optical birefringence measurement technology, we investigated the effect of magnetic field on the spatial ordering of hybr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00539
更新日期：2016-08-08 00:00:00
abstract：:Lignin is a natural, renewable biopolymer synthesized by plants. It is a macromolecule consisting of aromatic structures with high density of functional groups making it an ideal precursor for the design of sustainable biomaterials for applications such as drug delivery. The rational design of these materials requires...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00822
更新日期：2017-08-14 00:00:00
abstract：:Recent years have seen rapid growth in utilizing vegetable oils to derive a wide variety of polymers to replace petroleum-based polymers for minimizing environmental impact. Nonedible castor oil (CO) can be extracted from castor plants that grow easily, even in an arid land. CO is a promising source for developing sev...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01291
更新日期：2020-12-14 00:00:00
abstract：:Polymer conjugation increases an enzyme's circulation time and stability for use as a therapeutic agent, but this attachment indubitably affects its properties. Covalent attachment of multiple polyethylene glycol chains with sizes of either 2, 5, 10, or 20 kDa increases the molecular weight and hydrodynamic radius of ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1006954
更新日期：2010-12-13 00:00:00
abstract：:This paper describes the synthesis and cytotoxicity of poly(anhydride esters) that are composed of several salicylate derivatives, including halogenated salicylates, aminosalicylates, salicylsalicylic acid, and thiolsalicylic acid. The incorporation of these nonsteroidal antiinflammatory drugs (NSAIDs) into a biodegra...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049544+
更新日期：2005-01-01 00:00:00
abstract：:New applications in regenerative biotechnology require the ability to understand and control protein-surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein ads...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050446d
更新日期：2005-11-01 00:00:00
abstract：:Cancer cells have a large amount of ROS (reactive oxygen species) because of disturbed ROS homeostasis. Cancer cells therefore undertake redox adaptation to drive proliferation in tumor environments and even survive during anticancer treatment by upregulating endogenous antioxidants. As one of antioxidant defense syst...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01802
更新日期：2019-02-11 00:00:00
abstract：:The intermolecular distributions of amide groups within two commercial LMA pectins was studied after removal of the methyl esters followed by fractionation of the different populations by anion exchange chromatography. The populations obtained had almost equal degrees of amidation while the values of the degree of blo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050960j
更新日期：2006-06-01 00:00:00
abstract：:Hydrogels with pH-sensitive poly(acrylic acid) (PAAc) chains and biodegradable acryloyl-poly(-caprolactone)-2-hydroxylethyl methacrylate (AC-PCL-HEMA) chains were designed and synthesized. The morphology of hydrogel was observed by scanning electron microscopy. The degradation of the hydrogel in the presence of Pseudo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm7010328
更新日期：2008-04-01 00:00:00
abstract：:The influence of 2,2,6,6-tetrametylpiperidine-1-oxyl (TEMPO)-mediated oxidation of wood cellulose and the mechanical disintegration of oxidized cellulose in water on degree of polymerization determined by viscosity measurement (DP(v)) and the apparent length of the TEMPO-oxidized cellulose nanofibrils (TOCNs) was inve...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2017542
更新日期：2012-03-12 00:00:00
abstract：:Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801430x
更新日期：2009-05-11 00:00:00
abstract：:Biomimetic nanocomposites and scaffolds hold the key to a wide range of biomedical applications. Here we show, for the first time, a facile scheme of cofibrillizing pathogenic and functional amyloid fibrils via gold nanoparticles (AuNPs) and their applications against amyloidogenesis. This scheme was realized by β-she...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01359
更新日期：2017-12-11 00:00:00
abstract：:We have constructed a tandem gene expression cassette containing three Ralstonia eutropha poly[(R)-3-hydroxybutyrate] (PHB) synthesis genes under the control of the Pichia pastoris glyceraldehyde-3-phosphate promoter and the green fluorescent protein (Gfp) under the control of the P. pastoris alcohol oxidase promoter....
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0494141
更新日期：2005-03-01 00:00:00
abstract：:Nowadays, active targeting of nanotherapeutics is a challenging issue. Here, we propose a rational design of a ternary nanoassembly (SAP) composed of nonionic amphiphilic β-cyclodextrins (amphiphilic CD) incorporating pheophorbide (Pheo) as a phototherapeutic and an adamantanyl-folic acid conjugate (Ada-FA) to target ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00306
更新日期：2019-07-08 00:00:00
abstract：:Nanoparticles (NPs) with high drug loading and pH-responsivity were prepared by nanoprecipitation of a hydrophobic polymer-drug conjugate (PDC). The PDC, polylactide-graft-doxorubicin (PLA-g-DOX), was synthesized by azide-alkyne click reaction to transform acetylene-functionalized PLA into PLA-graft-aldehyde (PLA-g-AL...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401471p
更新日期：2014-02-10 00:00:00
abstract：:We report an enzyme-based method for the in situ entrapment of cells within a biopolymeric hydrogel matrix. Specifically, we used a calcium-independent microbial transglutaminase that is known to cross-link proteins and observed that it catalyzes the formation of gels from a pre-gel solution containing 10% gelatin and...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034145k
更新日期：2003-11-01 00:00:00
abstract：:Biocompatible photoresponsive materials are of interest for targeted drug delivery, tissue engineering, 2D and 3D protein patterning, and other biomedical applications. We prepared light degradable hydrogels using a natural alginate polysaccharide cross-linked with iron(III) cations. The "hard" iron(III) cations used ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300707a
更新日期：2012-08-13 00:00:00
abstract：:A novel pH-sensitive amphiphilic copolymer brush poly(methyl methacrylate-co-methacrylic acid)-b-poly(poly(ethylene glycol) methyl ether monomethacrylate) [P(MMA-co-MAA)-b-PPEGMA] was defined and synthesized by atom transfer radical polymerization (ATRP) technique. The molecular structures and characteristics of this ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm101058w
更新日期：2011-01-10 00:00:00
abstract：:The manufacture of biodegradable materials from agricultural sources is a real challenge, because of environmental concerns and the need to make full use of resources. Cottonseed is an important protein source that could be used in nonfood applications, as a substitute for synthetic polymers. For the first time, the v...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015525d
更新日期：2001-01-01 00:00:00
