Abstract:
:Thymic epithelial cells (TECs) are the main regulators of T lymphocyte development and selection, requiring a three-dimensional (3D) environment to properly perform these biological functions. The aim of this work was to develop a 3D culture substrate that allows the survival and proliferation of TECs. Thus, electrospun fibrous meshes (eFMs) were functionalized with fibronectin, one of the major extracellular matrix (ECM) proteins of the thymus. For that, highly porous eFMs were activated using oxygen plasma treatment followed by amine insertion, which allows the immobilization of fibronectin through EDC/NHS chemistry. The medullary TECs presented increased proliferation, viability, and protein synthesis when cultured on fibronectin-functionalized eFMs (FN-eFMs). These cells showed a spread morphology, with increased migration toward the inner layers of FN-eFMs and the production of thymic ECM proteins, such as collagen type IV and laminin. These results suggest that FN-eFMs are an effective substrate for supporting thymic cell cultures.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Silva CS,Pinto RD,Amorim S,Pires RA,Correia-Neves M,Reis RL,Alves NL,Martins A,Neves NMdoi
10.1021/acs.biomac.0c00933subject
Has Abstractpub_date
2020-12-14 00:00:00pages
4771-4780issue
12eissn
1525-7797issn
1526-4602journal_volume
21pub_type
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