Abstract:
:Heparin, a sulfated polysaccharide derived from animal sources, is the most commonly used parenteral anticoagulant drug, but it suffers from significant safety and supply issues. Herein, we describe the preparation of heparin mimetic homo- and copolymers via the reversible addition-fragmentation chain transfer (RAFT) polymerization in water of commercially available (non-carbohydrate) sulfonated and carboxylated monomers. The anticoagulant activities of the polymers were assessed by activated partial thromboplastin time (APTT), thrombin clotting time (TCT), and for the more promising polymers, thrombin generation, antifactor Xa, and antifactor IIa assays. Sulfonated homopolymers studied herein displayed low cytotoxicity and significant anticoagulant activity in APTT, TCT, and thrombin generation assays. In addition, copolymers of sodium styrenesulfonate and acrylic acid [poly(SSS-co-AA)] displayed unprecedented antifactor IIa activity. This study demonstrates the potential of RAFT polymers as alternative anticoagulants for biomedical applications.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Nahain AA,Ignjatovic V,Monagle P,Tsanaktsidis J,Vamvounis G,Ferro Vdoi
10.1021/acs.biomac.9b01688subject
Has Abstractpub_date
2020-02-10 00:00:00pages
1009-1021issue
2eissn
1525-7797issn
1526-4602journal_volume
21pub_type
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