Abstract：
:Owing to their diverse functions and tunable physicochemical properties, peptides are promising alternatives to the conventional gene delivery tools that are available for plant systems. However, peptide-mediated gene delivery is limited by low transfection efficiency in plants because of the insufficient cytosolic translocation of DNA cargo. Here, we report a dual peptide-based gene delivery system for the efficient transfection of plant callus cells. This system is based on the combination of an artificial peptide composed of cationic cell-penetrating and hydrophobic endosomal escape domains with a gene carrier peptide composed of amphiphilic cell-penetrating and cationic DNA-binding domains. Cellular internalization and transfection studies revealed that this dual peptide-based system enables more efficient transfection of callus cells than does a carrier peptide alone by enhancing the endocytic uptake and subsequent cytosolic translocation of a carrier peptide/DNA complex. The present strategy will expand the utility of peptide-mediated plant gene delivery for a wide range of applications and basic research.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Miyamoto T,Tsuchiya K,Numata Kdoi
10.1021/acs.biomac.0c00481subject
Has Abstractpub_date
2020-07-13 00:00:00pages
2735-2744issue
7eissn
1525-7797issn
1526-4602journal_volume
21pub_type
杂志文章abstract：:Characterization of poly-3-hydroxybutyric acid (PHB) and poly-3-hydroxybutyric-co-valeric acid (PHBV, 13% valerate) in chloroform was performed using size exclusion chromatography coupled to a multi-angle light scattering detector (SEC-MALS). Absolute molar mass averages, molar mass distribution, and the radius of gyr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030073l
更新日期：2004-03-01 00:00:00
abstract：:Alginate-based amphiphilic graft copolymers were synthesized by single electron transfer living radical polymerization (SET-LRP), forming stable micelles during polymerization induced self-assembly (PISA). First, alginate macroinitiator was prepared by partial depolymerization of native alginate, solubility modificati...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00470
更新日期：2015-07-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm7014224
更新日期：2008-05-01 00:00:00
abstract：:Biocatalytic pulp fibers were prepared using surface functionalization of bleached kraft pulp with amino groups (F) and further immobilization of a cross-linked glucose oxidase (G*) from Aspergillus niger. The cross-linked enzymes (G*) were characterized using X-ray spectroscopy, Fourier transform infrared spectroscop...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00865
更新日期：2016-10-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200899h
更新日期：2011-10-10 00:00:00
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journal_title：Biomacromolecules
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doi：10.1021/bm049735c
更新日期：2004-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060927c
更新日期：2007-02-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201712z
更新日期：2012-03-12 00:00:00
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journal_title：Biomacromolecules
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doi：10.1021/bm301997e
更新日期：2013-04-08 00:00:00
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journal_title：Biomacromolecules
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更新日期：2007-10-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9004854
更新日期：2009-08-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0502011
更新日期：2005-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800759g
更新日期：2008-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300545b
更新日期：2012-07-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01321
更新日期：2020-12-21 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00491
更新日期：2015-09-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2004912
更新日期：2011-10-10 00:00:00
abstract：:We report an enzyme-based method for the in situ entrapment of cells within a biopolymeric hydrogel matrix. Specifically, we used a calcium-independent microbial transglutaminase that is known to cross-link proteins and observed that it catalyzes the formation of gels from a pre-gel solution containing 10% gelatin and...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034145k
更新日期：2003-11-01 00:00:00
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journal_title：Biomacromolecules
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更新日期：2002-09-01 00:00:00
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journal_title：Biomacromolecules
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更新日期：2010-03-08 00:00:00
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更新日期：2005-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2019-01-14 00:00:00
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journal_title：Biomacromolecules
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更新日期：2014-02-10 00:00:00
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journal_title：Biomacromolecules
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更新日期：2006-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm000072i
更新日期：2001-04-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100209b
更新日期：2010-06-14 00:00:00
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journal_title：Biomacromolecules
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更新日期：2011-01-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00805
更新日期：2015-09-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015525d
更新日期：2001-01-01 00:00:00
