Abstract:
:Biocatalytic pulp fibers were prepared using surface functionalization of bleached kraft pulp with amino groups (F) and further immobilization of a cross-linked glucose oxidase (G*) from Aspergillus niger. The cross-linked enzymes (G*) were characterized using X-ray spectroscopy, Fourier transform infrared spectroscopy, dynamic scanning calorimetry, and dynamic light scattering. According to standard assays, the G* content on the resulting fibers (FG*) was of 11 mg/g of fiber, and enzyme activity was of 215 U/g. The results from confocal- and stimulated emission depletion microscopy techniques demonstrated that glucose oxidase do not penetrate the interlayers of fibers. The benefit of pulp fiber functionalization was evident in the present case, as the introduction of amino groups allowed the immobilization of larger amount of enzymes and rendered more efficient systems. Using the approach described on this paper, several advanced materials from wood pulp fibers and new bioprocesses might be developed by selecting the correct enzyme for the target applications.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Vega Erramuspe IB,Fazeli E,Näreoja T,Trygg J,Hänninen P,Heinze T,Fardim Pdoi
10.1021/acs.biomac.6b00865subject
Has Abstractpub_date
2016-10-10 00:00:00pages
3188-3197issue
10eissn
1525-7797issn
1526-4602journal_volume
17pub_type
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