Abstract：
:A new 320-member polymer library of end-modified poly(β-amino ester)s was synthesized. This library was chosen such that small differences to the structures of component backbone, side-chain, and end-group monomers could be systematically and simultaneously evaluated. The in vitro transfection efficacy and cytotoxicity of DNA nanoparticles formed from this library were assessed. This library approach not only enabled us to synthesize and test a large variety of structures rapidly but also provided us with a robust data set to analyze for the effect of small structural permutations to polymer chain structure. Small changes to the side chains, backbones, and end groups within this polymer library produced dramatic results, with transfection efficacy of CMV-Luc varying over 4 orders in a 96-well plate format. Increasing hydrophobicity of the base polymer backbone and side chain tended to increase transfection efficacy, but the most hydrophobic side chains and backbones showed the least requirement for a hydrophobic pair. Optimal PBAE formulations were superior to commercially available nonviral alternatives FuGENE HD and Lipofectamine 2000, enabling ~3-fold increased luminescence (2.2 × 10(6) RLU/well vs 8.1 × 10(5) RLU/well) and 2-fold increased transfection percentage (76.7% vs 42.9%) as measured by flow cytometry with comparable or reduced toxicity.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Sunshine JC,Akanda MI,Li D,Kozielski KL,Green JJdoi
10.1021/bm200807ssubject
Has Abstractpub_date
2011-10-10 00:00:00pages
3592-600issue
10eissn
1525-7797issn
1526-4602journal_volume
12pub_type
杂志文章abstract：:The elegant elasticity and toughness of muscles that are controlled by myofilament sliding, highly elastic springlike properties of titin, and Ca2+-induced conformational change of the troponin complex have been a source of inspiration to develop advanced materials for simulating elastic muscle motion. Herein, a highl...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01002
更新日期：2020-10-12 00:00:00
abstract：:As an aim toward developing biologically mimetic and functional nanofiber-based tissue engineering scaffolds, we demonstrated the encapsulation of a model protein, fluorescein isothiocyanate-conjugated bovine serum albumin (fitcBSA), along with a water-soluble polymer, poly(ethylene glycol) (PEG), within the biodegrad...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050743i
更新日期：2006-04-01 00:00:00
abstract：:In marine mussels (Mytilus), byssal threads are made in minutes from prefabricated smectic polymer liquid crystals by a process resembling reaction injection molding. The mesogens in these arrays are known to be natural block copolymers with rodlike collagen cores. Using atomic force microscopy, it was shown that thes...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049899t
更新日期：2004-07-01 00:00:00
abstract：:The supramolecular organization of self-assembled stoichiometric complexes formed by dodecyltrimethylammonium cations and ionized poly(gamma-glutamic) acid has been investigated in chloroform solution. Atomistic molecular dynamics simulations have been performed considering three different starting conformations for t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060927c
更新日期：2007-02-01 00:00:00
abstract：:Chitosan-dendrimer hybrids having various functional groups such as carboxyl, ester, and poly(ethylene glycol) groups were prepared successfully using dendrimer acetal by reductive N-alkylation. The synthetic procedure could be accomplished by one-step reaction without organic solvent. The degree of substitution of de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030021w
更新日期：2003-09-01 00:00:00
abstract：:Protein polymers (long-chain proteins in which a specific amino acid sequence "monomer" is repeated through the molecule) are found widely in nature, and these materials exhibit a diverse array of physical properties. One class of self-assembling proteins is hydrophobic-polar (HP) protein polymers capable of self-asse...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701131x
更新日期：2008-01-01 00:00:00
abstract：:We report a robust method to manufacture polyacrylamide-based functional hydrogel microspheres with readily tunable macroporous structures by utilizing a simple micromolding-based technique. Specifically, surface tension-induced droplet formation of aqueous solutions of chitosan and acrylamide in 2D-shaped micromolds ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00582
更新日期：2016-07-11 00:00:00
abstract：:X-ray analytical techniques are increasingly being used to study manuscripts and works of art on paper, whether with laboratory equipment or synchrotron sources. However, it is difficult to anticipate the impact of X-ray photons on paper- and cellulose-based artifacts, particularly due to the large variety of their co...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00512
更新日期：2020-07-13 00:00:00
abstract：:Aberrant expression of histone deacetylases (HDACs) is related to various types of cancer and is associated with increased proliferation of tumor cells. Hence, the detection of HDAC activities is of great significance for medical sciences as well as biological diagnostics. Herein, we report a hyperbranched polyester-b...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401548u
更新日期：2013-12-09 00:00:00
abstract：:Prevention of biofouling and microbial contamination of implanted biomedical devices is essential to maintain their functionality and biocompatibility. For this purpose, polypept(o)ide block copolymers have been developed, in which a protein-resistant polysarcosine (pSar) block is combined with a dopamine-modified pol...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00135
更新日期：2018-05-14 00:00:00
abstract：:Feasibility of using amphiphilic block copolymers composed of polylactide (PLA) and poly(ethylene oxide) (PEO) blocks for biomimetic surface modification of polylactide-based biomaterials for tissue engineering was investigated. PEO-b-PLA copolymers were deposited on the PLA surface from a solution in PEO-selective so...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900889b
更新日期：2010-01-11 00:00:00
abstract：:Although composed simply of glucose polymers, the starch granule is a complex, semicrystalline structure. Much of this complexity arises from the fact that the two primary enzymes of synthesis-starch synthase and starch-branching enzyme-exist as multiple isoforms. Each form has distinct properties and plays a unique r...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm000133c
更新日期：2001-07-01 00:00:00
abstract：:Self-assembled hydrogen-bonded networks of the polysaccharide pectin, a mechanically functional component of plant cell walls, have been of recent interest as biomimetic exemplars of physical gels, and the microrheological and strain-stiffening behaviors have been previously investigated. Despite this detailed rheolog...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00870
更新日期：2015-10-12 00:00:00
abstract：:Electrospinning is a versatile process used to prepare micro- and nano- sized fibers from various polymers dissolved in volatile solvents. In this report, cellulose and cellulose-heparin composite fibers are prepared from nonvolatile room temperature ionic liquid (RTIL) solvents by electrospinning. RTILs are extracted...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050837s
更新日期：2006-02-01 00:00:00
abstract：:Partially benzylamidated, amphipathic poly(gamma-glutamic acid) (BzPGA) was synthesized from poly(gamma-glutamic acid) (PGA) and benzylamine by direct amidation in dimethyl sulfoxide (DMSO). Benzylamine and PGA were heated in DMSO for 1 to 26 h at temperatures between 110 and 130 degrees C, producing derivatives of va...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0612182
更新日期：2007-05-01 00:00:00
abstract：:Self-assembly of protein-polymer bioconjugates presents an elegant strategy for controlling nanostructure and orientation of globular proteins in functional materials. Recent work has shown that genetic fusion of globular protein mCherry to an elastin-like polypeptide (ELP) yields similar self-assembly behavior to the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00147
更新日期：2018-07-09 00:00:00
abstract：:The mycobacterial cell wall is extraordinarily thick and tight consisting mainly of (1). long chain fatty acids, the mycolic acids, and (2). a unique polysaccharide, arabinogalactan (AG). These two chemical constituents are covalently linked through ester bonds. Minnikin (The Biology of the Mycobacteria; Academic: Lon...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034514c
更新日期：2004-05-01 00:00:00
abstract：:The inferior in vivo stability of micellar drugs has been a prime challenge for their application in targeted drug delivery. Here we report on novel galactose-decorated covalently cross-linked biodegradable micelles based on photo-cross-linkable poly(ethylene glycol)-b-poly(acryloyl carbonate)-b-poly(ε-caprolactone) (...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2006856
更新日期：2011-08-08 00:00:00
abstract：:A strategy to light-trigger ionic cross-linking of alginates by incorporating a photosensitive Ca2+ cage (nitr-T) is presented. Upon irradiation, free Ca2+ was released, and this caused gelation of the alginate solution. Addition of Ca2+ "on-demand" allowed us to obtain homogeneous alginate (ALG) gels using concentrat...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400022h
更新日期：2013-05-13 00:00:00
abstract：:Nanocellulose-based self-standing films are becoming a substrate for flexible electronics, diagnostics, and sensors. Strength and surface chemistry are vital variables for these film-based endeavors, the former is one of the assets of nanocellulose. To contribute to the latter, nanocellulose films are tuned with a sid...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01751
更新日期：2018-03-12 00:00:00
abstract：:We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700690p
更新日期：2007-11-01 00:00:00
abstract：:Synthetic and natural mucoadhesive biomaterials in optimized galenical formulations are potentially useful for the transmucosal delivery of active ingredients to improve their localized and prolonged effects. Chitosans (CS) have potent mucoadhesive characteristics, but the exact mechanisms underpinning such interactio...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5007954
更新日期：2014-10-13 00:00:00
abstract：:The application of nanoparticles comprising amphiphilic block copolymers for the delivery of drugs is a subject of great interest as they hold promise for more effective and selective therapies. In order to achieve this ambition, it is of critical importance to develop our understanding of the self-assembly mechanisms...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00169
更新日期：2020-06-08 00:00:00
abstract：:Materials with fungi-bioinspired surface have been designed to host ergosterol-binding polyene antibiotics and to release them via a competitive mechanism only when fungi are present in the medium. Silicone rubber (SR) surfaces were endowed with selective loading and fungi-triggered release of polyene antifungal agent...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500257s
更新日期：2014-05-12 00:00:00
abstract：:We have investigated the thermodynamic and dynamic behavior of multistranded β-lactoglobulin protein fibrils in water, by combining static, dynamic, and depolarized dynamic light scattering (SLS, DLS, DDLS), small angle neutron scattering (SANS), rheology, and cryogenic transmission electron microscopy (cryo-TEM). We ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301005w
更新日期：2012-10-08 00:00:00
abstract：:In this work, cell-responsive polysaccharide hydrogels were prepared by a simple procedure based on the sequential bioconjugation and cross-linking of the polysaccharide backbone with bioactive peptides and poly(ethylene glycol)-bis(thiol) (PEG-(SH)2), respectively. Using thiol-ene reactions, we successfully functiona...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501613u
更新日期：2015-03-09 00:00:00
abstract：:This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800234r
更新日期：2008-09-01 00:00:00
abstract：:Water-soluble, degradable polymers based on poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) with low cytotoxicity and good p-DNA transfection efficiency are highlighted in this article. To solve the nondegradability issue of PDMAEMA, new polymers based on DMAEMA and 5,6-benzo-2-methylene-1,3-dioxepane (BMDO) for g...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2015174
更新日期：2012-02-13 00:00:00
abstract：:Enzyme-responsive polypeptide vesicles have attracted considerable attention for precision theranostics because of their biocompatibility, biodegradability, and unique secondary conformation transition triggered by the catalytic actions of enzymes. These promising potentials of polypeptide vesicles could be limited in...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01251
更新日期：2020-12-14 00:00:00
abstract：:A well-defined fluorescent conjugated polyfluorene with pendant lactopyranosyl ligands was easily prepared through Cu(I)-catalyzed azide/alkyne "click" ligation and Suzuki coupling polymerization. As a fluorescent multivalent model system of glycoconjugates, the polymer was first used for studies of metal ion-mediated...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901165n
更新日期：2010-01-11 00:00:00
