Abstract:
:To provide for prolonged, site-specific delivery of basic fibroblast growth factor (bFGF) to the grafted skin in a convenient manner, biodegradable chitosan-gelatin microspheres containing bFGF were fabricated and incorporated into a porous chitosan-gelatin scaffold in this study. The microspheres are an integral part of the porous three-dimensional scaffolds, and their incorporation does not significantly affect the scaffold porosity and the pore size. The release kinetics of bFGF showed a fast release (23.7%) at the initial phase in the first 2 days, and the ultimate accumulated release was approximately 71.8% by day 14, indicating an extended time course for complete release. Human fibroblasts seeded on chitosan-gelatin scaffolds with and without bFGF-loaded chitosan-gelatin microspheres (bFGF-MS) were incubated in vitro for 2 weeks and showed that, compared to chitosan-gelatin scaffolds alone, the scaffolds with bFGF-MS significantly augmented the proliferation and glycosaminoglycan (GAG) synthesis of human fibroblasts. Moreover, real-time reversed transcribed polymerase chain reaction (RT-PCR) analysis for fibroblast-related extracellular matrix (ECM) gene markers demonstrated that the transcript level of laminin was markedly upregulated by about 9-fold. These results suggest that chitosan-gelatin scaffolds with bFGF-MS possess a promising potential as a tissue engineering scaffold to improve skin regeneration efficacy and to promote vascularization.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Liu H,Fan H,Cui Y,Chen Y,Yao K,Goh JCdoi
10.1021/bm061025esubject
Has Abstractpub_date
2007-05-01 00:00:00pages
1446-55issue
5eissn
1525-7797issn
1526-4602journal_volume
8pub_type
杂志文章abstract::This paper shows a simple and effective way to avoid the dissociation of multimeric enzymes by coating their surface with a large cationic polymer (e.g., polyethylenimine (PEI)) by ionic exchange. As model enzymes, glutamate dehydrogenase (GDH) from Thermus thermophilus and formate dehydrogenase (FDH) from Pseudomonas...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801162e
更新日期:2009-04-13 00:00:00
abstract::The formation of a suitable extracellular matrix (ECM) that promotes cell adhesion, organization, and proliferation is essential within biomaterial scaffolds for tissue engineering applications. In this work, short elastin mimetic peptide sequences, EM-19 and EM-23, were engineered to mimic the active motifs of human ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101214f
更新日期:2011-02-14 00:00:00
abstract::To investigate the influence of surface-functionalized substrates with nanostructures on the behaviors of mesenchymal stem cells, we conjugated bone morphogenetic protein 2 (BMP2) onto TiO(2) nanotubes with different diameter sizes of 30, 60, and 100 nm for in vitro study. Polydopamine was employed as the intermediate...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1014365
更新日期:2011-04-11 00:00:00
abstract::A new mathematical model is presented for the analysis of the substituent distribution in the polymer chain of polysaccharide derivatives. For the first time, the influence of substitution on the reactivity of neighbored monomer units is taken into account. The model was applied to various cellulose and amylose ethers...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm000093c
更新日期:2001-04-01 00:00:00
abstract::The lack of selectivity and low solubility of many chemotherapeutics impels the development of different biocompatible nanosized drug carriers. Amphiphilic block copolymers, composed of a hydrophilic and hydrophobic domain, show great potential because of their small size, large solubilizing power and loading capacity...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01252
更新日期:2016-01-11 00:00:00
abstract::The aggregation phenomena in aqueous solutions of hydrophobically modified (HM) chitosan, containing 4 mol % of n-dodecyl side chains, were studied by viscometry and fluorescence spectroscopy with pyrene as a probe. The results are compared with those for unmodified chitosan. Surprisingly, fluorescence data reveal the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm005649a
更新日期:2001-07-01 00:00:00
abstract::Reductively degradable biopolymers have emerged as a unique class of smart biomedical materials. Here, a functional coupling agent, cystamine diisocyanate (CDI), was designed to offer a facile access to reductively degradable biopolymers via polycondensation with various diols. CDI was readily obtained with a decent y...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01578
更新日期:2016-03-14 00:00:00
abstract::Tissue engineering approaches for peripheral nerve regeneration employ biodegradable scaffolds coupled with growth factors for improved performance in regeneration of large nerve injuries. Electrospun nanofibers provide a versatile platform for fabrication of scaffolds with extracellular matrix like architecture and i...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8012499
更新日期:2009-05-11 00:00:00
abstract::The interaction of iron with water-soluble polymer chitosan and monomer d-glucosamine is investigated by Mössbauer spectroscopy. The 4.2 K Mössbauer spectrum of Fe-water-soluble chitosan complex indicates the presence of a magnetic pattern and a quadrupole doublet, and analysis of the spectral data leads to the conclu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm020131n
更新日期:2003-05-01 00:00:00
abstract::The aim of this paper is to define optimal conditions for the extraction of chitin from shrimp shells. The kinetics of both demineralization and deproteinization with, in the latter case, the role of temperature are studied. The characterization of the residual calcium and protein contents, the molecular weights, and ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025602k
更新日期:2003-01-01 00:00:00
abstract::Relative to normal cells, tumor cells lack adequate capability of reactive oxygen scavenging. Thus, tumor cells can be selectively killed by increasing the concentration of reactive oxygen species in tumor tissue. In this report, we construct an integrated multifunctional polymeric nanoparticle which can selectively i...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01777
更新日期:2018-06-11 00:00:00
abstract::Owing to their diverse functions and tunable physicochemical properties, peptides are promising alternatives to the conventional gene delivery tools that are available for plant systems. However, peptide-mediated gene delivery is limited by low transfection efficiency in plants because of the insufficient cytosolic tr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00481
更新日期:2020-07-13 00:00:00
abstract::New silk-like recombinant proteins, [(AAG)(6)ASTGRGDSPAAS](n) and [(AG)(9)ASTGRGDSPAAS](n), with high cell adhesive activities were designed and produced from E. coli. These are recombinant proteins with characteristic sequences from the silk fibroin of a wild silkworm, Anaphe , and the cell adhesive region, including...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801439t
更新日期:2009-04-13 00:00:00
abstract::Developing new protein-based materials with a programmable assembly is of great scientific interest and technological importance. Inspired by nature's use of post-translational modifications (PTMs) to control the function and location of proteins, we have leveraged lipidation-the PTM of proteins with lipids-to synthes...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01380
更新日期:2020-02-10 00:00:00
abstract::These studies illustrate synthetic paths to covalently attach T1 and Φ11 bacteriophages (phages) to inert polymeric surfaces while maintaining the bacteriophage's biological activities capable of killing deadly human pathogens. The first step involved the formation of acid (COOH) groups on polyethylene (PE) and polyte...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400290u
更新日期:2013-05-13 00:00:00
abstract::Bacterial cellulose (BC) is a polysaccharide known as a suitable matrix for proper wound healing. To improve this ability, BC was functionalized with silk sericin (SS) that has cytoprotective and mitogenic effects. The composites obtained by solution impregnation were stabilized by hydrogen bonds, and SS could be rele...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00995
更新日期:2016-09-12 00:00:00
abstract::The time-dependent diffusion and mechanical properties of gelatin in solution, in the gel state, and during the sol/gel transition were determined using fluorescence recovery after photobleaching (FRAP) and rheology. The parameters in the experimental design were 2% w/w and 5% w/w gelatin concentration; 15, 20, and 25...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1008487
更新日期:2010-12-13 00:00:00
abstract::Nature shows excellent control over the mechanics of fibrous hydrogels by assembling protein fibers into bundles of well-defined dimensions. Yet, obtaining artificial materials displaying controlled bundling remains a challenge. Here, we developed genetically engineered protein-based polymers functionalized with hepar...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00276
更新日期:2016-06-13 00:00:00
abstract::Herein we report the synthesis of biodegradable hyperbranched polyglycerols (BHPGs) having acid-cleavable core structure by anionic ring-opening multibranching polymerization (ROMBP) of glycidol using initiators bearing dimethyl and cyclohexyl ketal groups. Five different multifunctional initiators carrying one to fou...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300959h
更新日期:2012-10-08 00:00:00
abstract::Bioresponsive polymers can enable the development of more effective drug delivery vehicles and medical materials. Acetal-modified polysaccharides allow pH-triggered solubility switching in a versatile and effective manner, but prior work has been limited to a combination of acyclic alkoxyisopropyl and cyclic isopropyl...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00215
更新日期:2019-05-13 00:00:00
abstract::Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801430x
更新日期:2009-05-11 00:00:00
abstract::Many biological processes are regulated by reversible binding events, with these interactions between macromolecules representing the core of dynamic chemistry. As such, any attempt to gain a better understanding of such interactions, which would pave the way to the extrapolation of natural designs to create new advan...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01103
更新日期:2015-10-12 00:00:00
abstract::Thermoresponsive polymers with lower critical solution temperatures (LCSTs) are of significant interest for a wide range of applications from sensors to drug delivery vehicles. However, the most widely investigated LCST polymers have nondegradable backbones, limiting their applications in vivo or in the environment. D...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00899
更新日期:2020-09-14 00:00:00
abstract::There is a distinct need for antimicrobial compounds that can act at surfaces without leaching into the environment. Such materials should be easy to synthesize, be easy to apply to surfaces, and display reasonable levels of antimicrobial and antifungal activity. Here we describe such a surface-active compound and dem...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060476w
更新日期:2006-10-01 00:00:00
abstract::The new combinations of monomers presented in this work were evaluated in order to create an elastic material for potential application in soft tissue engineering. Thermoplastic elastomers (TPE) of trimethylene carbonate (TMC) with L-lactide (LLA) and 1,5-dioxepan-2-one (DXO) have been synthesized using a cyclic five-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060081c
更新日期:2006-05-01 00:00:00
abstract::The coefficient of thermal expansion (CTE) of cellulose nanocrystal (CNC) films was characterized using novel experimental techniques complemented by molecular simulations. The characteristic birefringence exhibited by CNC films was utilized to calculate the in-plane CTE of self-organized and shear-oriented self-stand...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400794e
更新日期:2013-08-12 00:00:00
abstract::This study highlights the advantages of chondroitin sulfate (CS) as a sublayer combining selective low-fouling properties, low-platelet adhesion and pro-adhesive properties on endothelial cells, making CS promising for vascular graft applications. These properties were evaluated by comparing CS with well-known low-fou...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm5003762
更新日期:2014-07-14 00:00:00
abstract::A series of poly(vinyl alcohol) amphiphilic derivatives have been prepared to obtain polymeric aggregates in aqueous phase holding thermodynamic instability. The aim was to evaluate their ability to interact with tumor cells eliciting selective cytotoxicity. The poly(vinyl alcohol) derivatives were prepared by partial...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0504328
更新日期:2005-09-01 00:00:00
abstract::Collagen fibrils were obtained in vitro by aggregation from acid-soluble type I collagen at different initial concentrations and with the addition of decorin core or intact decorin. All specimens were observed by scanning electron microscopy and atomic force microscopy. In line with the findings of other authors, lack...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070091t
更新日期:2007-07-01 00:00:00
abstract::Shear flow is indirectly implicated in amyloid formation in vitro. Despite the association between amyloid fibrils and disease, and the prevalence of flow in physiological systems, the effect of this parameter is uncharacterized. We designed a novel Couette cell to quantitatively investigate shear exposure during fibr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0505078
更新日期:2006-01-01 00:00:00