Abstract:
:Shear flow is indirectly implicated in amyloid formation in vitro. Despite the association between amyloid fibrils and disease, and the prevalence of flow in physiological systems, the effect of this parameter is uncharacterized. We designed a novel Couette cell to quantitatively investigate shear exposure during fibrillogenesis. Amyloid formation by beta-lactoglobulin was monitored in situ with real-time fluorescence measurements across a range of shear rates. We demonstrate shear-induced aggregation of spheroidal seed-like species. These seeds enhance fibril formation in native beta-lactoglobulin, thereby demonstrating that shear flow generates an amyloidogenic precursor. Furthermore, preformed fibrils are degraded by exposure to high shear rates. Our results have implications for the mechanism of amyloid formation in physiological flow conditions.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Hill EK,Krebs B,Goodall DG,Howlett GJ,Dunstan DEdoi
10.1021/bm0505078keywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
10-3issue
1eissn
1525-7797issn
1526-4602journal_volume
7pub_type
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