Abstract:
:alpha-Conotoxins possess a conserved four-cysteine framework and disulfide linkages (C(1)(-)(3), C(2)(-)(4)) that fold toward the globular conformation with absolute fidelity. Despite the presence of a similar conserved set of cysteine framework, chi/lambda-conotoxins adopt an alternate disulfide-pairing (C(1)(-)(4), C(2)(-)(3)) and its consequent ribbon conformation, exhibiting distinct biological activities from alpha-conotoxins. chi/lambda-Conotoxin CMrVIA (VCCGYKLCHOC-COOH) isolated from the venom of Conus marmoreus natively exists in the ribbon conformation and induces seizures in mice at a potency that is of three orders higher than the non-native globular form. We have chemically synthesized two isoforms of CMrVIA conotoxin in the ribbon and globular conformation and determined their structures by (1)H NMR spectroscopy. The ribbon (PDB ID 2B5P) and globular conformations (PBD ID 2B5Q) were calculated to have paired-wise backbone RMSDs of 0.48 +/- 0.1 and 0.58 +/- 0.1 A respectively. Unlike the native globular alpha-conotoxins, the globular canonical form of CMrVIA chi/lambda-conotoxin exhibited heterogeneity in its solution structure as noted by the presence of minor conformers and poorer RMSD of structure calculation. Paired-wise backbone comparison between the native ribbon and the non-native globular form of CMrVIA conotoxin revealed an RMSD of 4.73 A, emphasizing their distinct conformational differences. These structural data are essential for the understanding of the structure-function activity of chi/lambda-conotoxins, as well as unraveling the folding propensities of these short peptide toxins.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Kang TS,Jois SD,Kini RMdoi
10.1021/bm060269wsubject
Has Abstractpub_date
2006-08-01 00:00:00pages
2337-46issue
8eissn
1525-7797issn
1526-4602journal_volume
7pub_type
杂志文章abstract::The storage and transport of cells is a fundamental technology which underpins cell biology, biomaterials research, and emerging cell-based therapies. Inspired by antifreeze and ice-binding proteins in extremophiles, macromolecular (polymer) cryoprotectants are emerging as exciting biomaterials to enable the reduction...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00591
更新日期:2020-07-13 00:00:00
abstract::The maintenance of hepatocyte phenotype and functions remains as a great challenge in the generation of functional liver tissue and in vitro model for drug metabolism studies. The use of hepatocyte coculture systems plays essential roles in the establishment of cell-cell and cell-extracellular matrix communications si...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401926k
更新日期:2014-03-10 00:00:00
abstract::Ionic liquids (ILs) are composed only of ions. Of special interest to this review are those where at least one ion (the cation) is organic and whose melting points are below or not far above room temperature. ILs are designated as "green" solvents because they have extremely low vapor pressure, are non-inflammable, an...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm070062i
更新日期:2007-09-01 00:00:00
abstract::Antibacterial nanodevices could bring coatings of plastic materials and wound dressings a big step forward if the release of the antibacterial agents could be triggered by the presence of the bacteria themselves. Here, we show that novel hyaluronic acid (HA)-based nanocapsules containing the antimicrobial agent polyhe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm302003m
更新日期:2013-04-08 00:00:00
abstract::The dynamics of silk protein in the presence and absence of water has been investigated by dielectric relaxation spectroscopy (DRS). The silk fibroin film cast from its water solution contains 4-7 wt % bound water molecules, which can be removed by dehydration at 165 °C. Temperature and frequency scans were performed ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1008316
更新日期:2010-10-11 00:00:00
abstract::Inspired by tea stains, plant polyphenolic tannic acid (TA) was beneficially employed as the primer anchor for functional polymer brushes. The brominated TA (TABr) initiator primer was synthesized by partial modification of TA with alkyl bromide functionalities. TABr with trihydroxyphenyl moieties can readily anchor o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501623c
更新日期:2015-03-09 00:00:00
abstract::Light-harvesting antenna core (LH1-RC) complexes isolated from Rhodoseudomonas palustris were self-assembled on a gold electrode modified with self-assembled monolayers (SAMs) of the alkanethiols NH2(CH2)nSH, n = 2, 6, 8, 11; HOOC(CH2)7SH; and CH3(CH2)7SH, respectively. Adsorption of the LH1-RC complexes on the SAMs d...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070352z
更新日期:2007-08-01 00:00:00
abstract::An efficient process for the synthesis of degradable hydrogels containing octa-betaine ester polyhedral oligomeric silsesquioxane (POSS) through efficient thiol-ene and Menschutkin click reactions was developed. The hydrogels exhibited a yield strength of 0.36 MPa and a compressive modulus of 4.38 MPa and displayed ex...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00530
更新日期:2020-09-14 00:00:00
abstract::Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2009075
更新日期:2011-09-12 00:00:00
abstract::The unsatisfied results of cancer therapy are caused by many issues and metastasis of cancer cells is one of the major challenge. It has been reported that inhibiting the SDF1/CXCR4 interaction can significantly reduce the metastasis of breast cancer cells to regional lymph nodes and lung. Herein, a nanogel system equ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00208
更新日期:2017-06-12 00:00:00
abstract::The fate of intravenously injected nanoparticles (NPs) is significantly affected by nano-protein interaction and corona formation. However, such an interaction between NPs and digestive enzymes occurring in the gastrointestinal tract (GIT) and its impacts on epithelial cell uptake are little known. We synthesized the ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00175
更新日期:2019-04-08 00:00:00
abstract::We utilize an aqueous extract of fish proteins (FPs) as a coating for minimizing the adsorption of fibrinogen (Fg) and human serum albumin (HSA). The surfaces include stainless steel (SS), gold (Au), silicon dioxide (SiO(2)), and poly(styrene) (PS). The adsorption processes (kinetics and adsorbed mass) are followed by...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900589r
更新日期:2009-10-12 00:00:00
abstract::Exendin-4 (EX4), a glucagon-like peptide-1 receptor (GLP-1R) agonist that regulates blood glucose levels, has been used in the management of type-2 diabetes mellitus. EX4 can be PEGylated to improve its antidiabetic effects by enhancing its stability and extending the circulation half-life. Here, to determine whether ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01756
更新日期:2016-03-14 00:00:00
abstract::As a result of improved relevance to in vivo physiology, in vitro studies are increasingly performed in diverse, three-dimensional (3D) biomaterials. However, material-cell type pairing effects on cytokine availability remain unclear. We cultured five cell types in agarose, alginate, collagen, Matrigel, or RGD-functio...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01469
更新日期:2017-03-13 00:00:00
abstract::The clinical application of holmium-loaded poly(L-lactic acid) (PLLA) microspheres for the radionuclide treatment of liver malignancies requires in depth understanding of the degradation characteristics of the microspheres. To this end, an in-vitro degradation study was conducted. PLLA-microspheres with and without Ho...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060230r
更新日期:2006-07-01 00:00:00
abstract::Biological composites are typically based on an adhesive matrix that interlocks rigid reinforcing elements in fiber composite or brick-and-mortar assemblies. In nature, the adhesive matrix is often made up of proteins, which are also interesting model systems, as they are unique among polymers in that we know how to e...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501514w
更新日期:2015-01-12 00:00:00
abstract::Enzymatic adsorption and degradation of three types of aliphatic polyester films and two types of polyolefin films by the extracellular PHB depolymerase from Alcaligenes faecalis T1 have been studied by using a quartz crystal microbalance (QCM) technique. Hydrolysis of poly[(R)-3-hydroxybutyrate] was quantitatively fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0000844
更新日期:2001-04-01 00:00:00
abstract::N-Acetyllactosamine (LacNAc; Galβ4GlcNAc) is a typical disaccharide ligand of galectins. The most abundant members of these human lectins, galectin-1 (Gal-1) and galectin-3 (Gal-3), participate in a number of pathologies including cancerogenesis and metastatic formation. In this study, we synthesized a series of fifte...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01370
更新日期:2020-02-10 00:00:00
abstract::First generation, photocrosslinkable dendrimers consisting of natural metabolites (i.e., succinic acid, glycerol, and beta-alanine) and nonimmunogenic poly(ethylene glycol) (PEG) were synthesized divergently in high yields using ester and carbamate forming reactions. Aqueous solutions of these dendrimers were photocro...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800658x
更新日期:2008-10-01 00:00:00
abstract::Enzymatic ring-opening copolymerization of 5-benzyloxy-trimethylene carbonate (BTMC) and 1,4-dioxan-2-one (DON) was investigated for the first time. Immobilized porcine pancreas lipase (IPPL) on silica particles was selected to perform the copolymerization. A series of novel biodegradable copolymers with different com...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060070j
更新日期:2006-08-01 00:00:00
abstract::The effect of increasing water composition on the rheological and microstructural behavior of a ternary cellulose acetate (CA)/N,N-dimethylacetamide (DMA)/water system is examined. Addition of water to the CA/DMA system results in enhanced steady shear viscosity and dynamic viscoelastic properties and ultimately to ph...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0611681
更新日期:2007-05-01 00:00:00
abstract::Bovine hemoglobin (Hb) was encapsulated inside polymer vesicles (polymersomes) to form polymersome encapsulated Hb (PEH) dispersions. PEH particles are 100% surface PEGylated with longer PEG chains and possess thicker hydrophobic membranes as compared to conventional liposomes. Polymersomes were self-assembled from po...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0501454
更新日期:2005-07-01 00:00:00
abstract::Cationic kraft lignin (CKL) macromolecules were produced via polymerizing kraft lignin (KL) with [2-(acryloyloxy)ethyl]trimethylammonium chloride (ATAC) or [2-(methacryloyloxy)ethyl]trimethylammonium methyl sulfate (METAM). Despite slightly different charge densities (2.3-2.5 mmol/g) of CKL, lignin-METAM (KL-METAM) ha...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01016
更新日期:2019-10-14 00:00:00
abstract::We study the self-assembly of genetically engineered protein-based triblock copolymers consisting of a central pH-responsive silk-like middle block (S(H)n, where S(H) is a silk-like octapeptide, (GA)3GH and n is the number of repeats) flanked by hydrophilic random coil outer blocks (C2). Our previous work has already ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500826y
更新日期:2014-09-08 00:00:00
abstract::Novel biodegradable poly(carbonate ester)s with photolabile protecting groups were synthesized by ring-opening copolymerization of L-lactide (LA) with 5-methyl-5-(2-nitro-benzoxycarbonyl)-1,3-dioxan-2-one (MNC) with diethyl zinc (Et2Zn) as catalyst. The poly(L-lactide-co-5-methyl-5-carboxyl-1,3-dioxan-2-one) (P(LA-co-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700906k
更新日期:2008-01-01 00:00:00
abstract::Saccharide-functionalized shell cross-linked (SCK) polymer micelles designed as polyvalent nanoscaffolds for selective interactions with receptors on Gram negative bacteria were constructed from mixed micelles composed of poly(acrylic acid-b-methyl acrylate) and mannosylated poly(acrylic acid-b-methyl acrylate). The m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0344710
更新日期:2004-05-01 00:00:00
abstract::Feasibility of using amphiphilic block copolymers composed of polylactide (PLA) and poly(ethylene oxide) (PEO) blocks for biomimetic surface modification of polylactide-based biomaterials for tissue engineering was investigated. PEO-b-PLA copolymers were deposited on the PLA surface from a solution in PEO-selective so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900889b
更新日期:2010-01-11 00:00:00
abstract::The aim of this paper is to define optimal conditions for the extraction of chitin from shrimp shells. The kinetics of both demineralization and deproteinization with, in the latter case, the role of temperature are studied. The characterization of the residual calcium and protein contents, the molecular weights, and ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025602k
更新日期:2003-01-01 00:00:00
abstract::A novel pH-sensitive amphiphilic copolymer brush poly(methyl methacrylate-co-methacrylic acid)-b-poly(poly(ethylene glycol) methyl ether monomethacrylate) [P(MMA-co-MAA)-b-PPEGMA] was defined and synthesized by atom transfer radical polymerization (ATRP) technique. The molecular structures and characteristics of this ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101058w
更新日期:2011-01-10 00:00:00
abstract::A first attempt to attenuate the quorum sensing (QS) of a marine heterotroph microorganism, Vibrio fischeri , using signal molecule-sequestering polymers (SSPs) is presented. A set of rationally designed polymers with affinity toward a signal molecule of V. fischeri , N-(beta-ketocaproyl)-l-homoserine lactone (3-oxo-C...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901451j
更新日期:2010-04-12 00:00:00