Abstract:
:The aim of this paper is to define optimal conditions for the extraction of chitin from shrimp shells. The kinetics of both demineralization and deproteinization with, in the latter case, the role of temperature are studied. The characterization of the residual calcium and protein contents, the molecular weights, and degrees of acetylation (DA) allows us to propose the optimal conditions as follows. The demineralization is completely achieved within 15 min at ambient temperature in an excess of HCl 0.25 M (with a solid-to-liquid ratio of about 1/40 (w/v)). The deproteinization is conveniently obtained in NaOH 1 M within 24 h at a temperature close to 70 degrees C with no incidence on the molecular weight or the DA. In these conditions, the residual content of calcium in chitin is below 0.01%, and the DA is almost 95%.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Percot A,Viton C,Domard Adoi
10.1021/bm025602kkeywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
12-8issue
1eissn
1525-7797issn
1526-4602journal_volume
4pub_type
杂志文章abstract::This paper describes the synthesis and characterization of polymer-peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacte...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500168s
更新日期:2014-06-09 00:00:00
abstract::The carboxyl-terminal domain (CTD, residues 146-231) of the HIV-1 capsid (CA) protein plays an important role in the CA-CA dimerization and viral assembly of the human immunodeficiency virus type 1. Disrupting the native conformation of the CA is essential for blocking viral capsid formation and viral replication. Thu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801151r
更新日期:2009-02-09 00:00:00
abstract::Spider dragline silk with its superlative tensile properties provides an ideal system to study the relationship between morphology and mechanical properties of a structural protein. Accordingly, we synthesized two hybrid multiblock copolymers by condensing poly(alanine) [(Ala)(5)] blocks of the structural proteins (sp...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060199t
更新日期:2006-08-01 00:00:00
abstract::A subtle but highly pertinent factor in the self-assembly of hierarchical nanostructures is the kinetic landscape. Self-assembly of a hierarchical multicomponent system requires the intricate balance of noncovalent interactions on a similar energy scale that can result in several self-assembly processes occurring at d...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01831
更新日期:2017-03-13 00:00:00
abstract::In situ forming reduction-sensitive degradable nanogels were designed and developed based on poly(ethylene glycol)-b-poly(2-(hydroxyethyl) methacrylate-co-acryloyl carbonate) (PEG-P(HEMA-co-AC)) block copolymers for efficient loading as well as triggered intracellular release of proteins. PEG-P(HEMA-co-AC) copolymers ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400206m
更新日期:2013-04-08 00:00:00
abstract::This study presents an efficient and systematic approach to synthesize bioapplicable porous hollow polymeric capsules (HPCs). The hydroxyl-functionalized nanoporous polymers with hollow capsular shapes could be generated via the moderate Friedel-Crafts reaction without using any hard or soft template. The numerous pri...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01520
更新日期:2020-12-17 00:00:00
abstract::A novel pH and temperature sensitive block copolymer was prepared by adding pH sensitive moiety to temperature sensitive block copolymer. This block copolymer solution showed a reversible sol-gel transition by a small pH change in the range of pH 7.4-8.0 and also by the temperature change in the region of body tempera...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050521k
更新日期:2005-11-01 00:00:00
abstract::Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment. The starting suspensions used at ∼6 wt % CNC separated in...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501629g
更新日期:2014-12-08 00:00:00
abstract::The use of polyelectrolyte surface-modified Cowpea mosaic virus (CPMV) for the templated synthesis of narrowly dispersed gold nanoparticles is described. The cationic polyelectrolyte, poly(allylamine) hydrochloride (PAH), is electrostatically bound to the external surface of the virus capsid; the polyelectrolyte promo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200499v
更新日期:2011-07-11 00:00:00
abstract::The formation of calcite prism architecture in the prismatic layer of the mollusk shell involves the participation of a number of different proteins. One protein family, Asprich, has been identified as a participant in amorphous calcium carbonate stabilization and calcite architecture in the prismatic layer of the mol...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100738r
更新日期:2010-10-11 00:00:00
abstract::The gold standard for implant metals is titanium, and coatings such as collagen-I, RGD-peptide, chondroitin sulfate, and calcium phosphate have been used to modify its biocompatibility. We investigated how titanium coated with pectins, adaptable bioactive plant polysaccharides with anti-inflammatory effects, supports ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800356b
更新日期:2008-09-01 00:00:00
abstract::Adhesive hydrogels were prepared by copolymerizing dopamine methacrylamide with either acrylic acid (AAc) or N-(3-aminopropyl)methacrylamide hydrochloride (APMH). The effect of incorporating the anionic and cationic side chains on the oxidation state of catechol was characterized using the FOX assay to track the produ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01311
更新日期:2018-05-14 00:00:00
abstract::Feasibility of using amphiphilic block copolymers composed of polylactide (PLA) and poly(ethylene oxide) (PEO) blocks for biomimetic surface modification of polylactide-based biomaterials for tissue engineering was investigated. PEO-b-PLA copolymers were deposited on the PLA surface from a solution in PEO-selective so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900889b
更新日期:2010-01-11 00:00:00
abstract::Enzymatic ring-opening copolymerization of 5-benzyloxy-trimethylene carbonate (BTMC) and 1,4-dioxan-2-one (DON) was investigated for the first time. Immobilized porcine pancreas lipase (IPPL) on silica particles was selected to perform the copolymerization. A series of novel biodegradable copolymers with different com...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060070j
更新日期:2006-08-01 00:00:00
abstract::Photodynamic therapy (PDT) has attracted widespread attention in recent years as a noninvasive and highly selective approach for cancer treatment. We have previously reported a significant increase in the 90-day complete response rate when tumor-bearing mice are treated with the epidermal growth factor receptor (EGFR)...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00274
更新日期:2017-06-12 00:00:00
abstract::It is recognized that topographical features such as ridges and grooves can dramatically influence cell phenotype, motivating the development of substrates with precisely biomimetic topography for study of the influence on cultured cells. Intestinal basement membrane topography has been precisely replicated using plas...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100209b
更新日期:2010-06-14 00:00:00
abstract::Enzymatic adsorption and degradation of three types of aliphatic polyester films and two types of polyolefin films by the extracellular PHB depolymerase from Alcaligenes faecalis T1 have been studied by using a quartz crystal microbalance (QCM) technique. Hydrolysis of poly[(R)-3-hydroxybutyrate] was quantitatively fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0000844
更新日期:2001-04-01 00:00:00
abstract::Fluorescence-tagged coniferyl alcohols, coniferyl alcohol γ-coupled by ethylenediamine spacers to dimethylaminocoumarin or nitrobenzofuran fluorophores, were tested as photoprobes to study the oxidase-mediated polymerization of monolignols. The fluorescent coniferyl alcohol derivatives readily underwent peroxidase-cat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200136x
更新日期:2011-05-09 00:00:00
abstract::The basic physicochemical properties that determine the distribution and fate of synthetic macromolecules in living cells were characterized using fluorescently labeled HPMA (N-(2-hydroxypropyl)methacrylamide) copolymers. Twelve different classes of water-soluble copolymers were created by incorporating eight differen...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801514x
更新日期:2009-07-13 00:00:00
abstract::The objective of this study was to explore the use of reverse thermo-responsive (RTG) polymers for generating implants at their site of performance, following minimally invasive surgical procedures. Aiming at combining syringability and enhanced mechanical properties, a new family of injectable RTG-displaying polymers...
journal_title:Biomacromolecules
pub_type:
doi:10.1021/bm0495250
更新日期:2005-05-01 00:00:00
abstract::A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell line...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00776
更新日期:2016-10-10 00:00:00
abstract::We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200780r
更新日期:2011-10-10 00:00:00
abstract::Phase separation mechanisms and kinetics were studied using small-angle light scattering in a gelatin/maltodextrin system where phase separation could be studied in both liquid and gelled states. Nucleation and growth or spinodal decomposition occurred, depending on the quench depth. The transition between the two mec...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025501m
更新日期:2002-07-01 00:00:00
abstract::Poly( n-butyl methacrylate) (PBMA) or poly( n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization "from the surface". The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight seconda...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00030
更新日期:2019-06-10 00:00:00
abstract::Ocular applications of polymeric materials have been widely investigated for medical diagnostics, treatment, and vision improvement. The human eye is a vital organ that connects us to the outside world so when the eye is injured, infected, or impaired, it needs immediate medical treatment to maintain clear vision and ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01525
更新日期:2021-01-06 00:00:00
abstract::On-line size-exclusion chromatography monitoring of potato phosphorylase catalyzed amylose synthesis--starting from alpha-D-glucose-1-P and maltohexaose--revealed rather monodisperse amylose populations. In the presence of lipids, amylose-lipid complexes spontaneously formed and precipitated. They were recovered by ce...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0502011
更新日期:2005-09-01 00:00:00
abstract::The formation of a microvasculature is regulated in large part by cell-cell interactions. Ephrins and their Eph receptors mediate cell adhesion, repulsion, and migration, all critical processes in angiogenesis. (1) Here we use a covalently immobilized ephrinA1, conjugated to poly(ethylene glycol), to induce vessel for...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200492h
更新日期:2011-07-11 00:00:00
abstract::These studies illustrate synthetic paths to covalently attach T1 and Φ11 bacteriophages (phages) to inert polymeric surfaces while maintaining the bacteriophage's biological activities capable of killing deadly human pathogens. The first step involved the formation of acid (COOH) groups on polyethylene (PE) and polyte...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400290u
更新日期:2013-05-13 00:00:00
abstract::A novel strategy for artificial regulation system of gene expression applying the specific molecular recognition between carbohydrate and lectin is proposed. Plasmid-lactose conjugates (pActin-lactose and pGFP-lactose) prepared via diazocoupling maintained the transcription activity with T7 RNA polymerase. Gel-shift a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050255a
更新日期:2005-09-01 00:00:00
abstract::The average pore size of hydrated dextran microspheres is derived from rheological and protein release data. The microspheres were prepared by cross-linking an aqueous solution of methacrylated dextran emulsified in a continuous poly(ethylene glycol) phase. The rheological data were obtained using a novel micromanipul...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm005574a
更新日期:2000-01-01 00:00:00