Abstract：
:The storage and transport of cells is a fundamental technology which underpins cell biology, biomaterials research, and emerging cell-based therapies. Inspired by antifreeze and ice-binding proteins in extremophiles, macromolecular (polymer) cryoprotectants are emerging as exciting biomaterials to enable the reduction and/or replacement of conventional cryoprotective agents such as DMSO. Here, we critically study post-thaw cellular outcomes upon addition of macromolecular cryoprotectants to provide unambiguous evidence that post-thaw culturing time and a mixture of assays are essential to claim a positive outcome. In particular, we observe that only measuring the viability of recovered cells gives false positives, even with non-cryoprotective polymers. Several systems gave apparently high viability but very low total cell recovery, which could be reported as a success but in practical applications would not be useful. Post-thaw culture time is also shown to be crucial to enable apoptosis to set in. Using this approach we demonstrate that polyampholytes (a rapidly emerging class of cryoprotectants) improve post-thaw outcomes across both measures, compared to poly(ethylene glycol), which can give false positives when only viability and short post-thaw time scales are considered. This work will help guide the discovery of new macromolecular cryoprotectants and ensure materials which only give positive results under limited outcomes can be quickly identified and removed.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Murray KA,Gibson MIdoi
10.1021/acs.biomac.0c00591subject
Has Abstractpub_date
2020-07-13 00:00:00pages
2864-2873issue
7eissn
1525-7797issn
1526-4602journal_volume
21pub_type
杂志文章abstract：:An underwater bioadhesive generally comprises a multiprotein complex that provides a molecular basis for self-assembly. We report here a new class of self-assembling peptide inspired by a 20 kDa barnacle cement protein. Studies on the chemically synthesized 24-residue peptide have revealed that (1) it underwent irreve...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0612236
更新日期：2007-06-01 00:00:00
abstract：:Amphiphilic polymer conetworks (APCNs) are materials with a very large interface between their hydrophilic and hydrophobic phases due to their nanophase-separated morphologies. Proteins were found to enrich in APCNs by up to 2 orders of magnitude when incubated in aqueous protein solutions, raising the question of the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1015877
更新日期：2011-05-09 00:00:00
abstract：:The interaction between DNA and different types of amino acid-based cationic surfactants was investigated. Particular attention was directed to determine the extent of influence of surfactant head-group geometry toward tuning the interaction behavior of these surfactants with DNA. An overview is obtained by gel retard...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8000765
更新日期：2008-07-01 00:00:00
abstract：:Oral delivery remains a challenge for poorly permeable hydrophilic macromolecules. Poly(amido amine) (PAMAM) dendrimers have shown potential for their possible oral delivery. Transepithelial transport of carboxyl-terminated G3.5 and amine-terminated G4 PAMAM dendrimers was assessed using isolated rat jejunal mucosae m...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5004465
更新日期：2014-08-11 00:00:00
abstract：:A heterofunctional support for enzyme immobilization may be defined as that which possesses several distinct functionalities on its surface able to interact with a protein. We will focus on those supports in which a final covalent attachment between the enzyme and the support is achieved. Heterofunctionality sometimes...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm400762h
更新日期：2013-08-12 00:00:00
abstract：:Cardiovascular diseases plague human health because of the lack of transplantable small-diameter blood vessel (SDBV) grafts. Although expanded polytetrafluoroethylene (ePTFE) has the potential to be used as a biocompatible material for SDBV grafts, long-term patency is still the biggest challenge. As discussed in this...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00897
更新日期：2020-09-14 00:00:00
abstract：:Antibacterial nanodevices could bring coatings of plastic materials and wound dressings a big step forward if the release of the antibacterial agents could be triggered by the presence of the bacteria themselves. Here, we show that novel hyaluronic acid (HA)-based nanocapsules containing the antimicrobial agent polyhe...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm302003m
更新日期：2013-04-08 00:00:00
abstract：:The aggregation phenomena in aqueous solutions of hydrophobically modified (HM) chitosan, containing 4 mol % of n-dodecyl side chains, were studied by viscometry and fluorescence spectroscopy with pyrene as a probe. The results are compared with those for unmodified chitosan. Surprisingly, fluorescence data reveal the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm005649a
更新日期：2001-07-01 00:00:00
abstract：:Block copolypeptides, which combine the self-assembly of block copolymers and the highly ordered 3D structures of proteins, are potential candidates for novel supramolecular structures and biotech applications, such as biosensors, tissue engineering, and selective drug delivery. The synthesis of model block copolypept...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0497217
更新日期：2004-09-01 00:00:00
abstract：:Sulfated glycosaminoglycans (GAGs) can direct cellular processes by interacting with proteins of the extracellular matrix (ECM). In this study we characterize the interaction profiles of chemically sulfated hyaluronan (HA) and chondroitin sulfate (CS) derivatives with bone morphogenetic protein-2 (BMP-2) and investiga...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5006855
更新日期：2014-08-11 00:00:00
abstract：:A drying environment is always a proposition faced by dynamic living organisms using water, which are driven by biopolymer-based micro- and macrostructures. Here, we introduce a drying process for aqueous liquid crystalline (LC) solutions composed of biopolymer with extremely high molecular weight components such as p...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00302
更新日期：2016-06-13 00:00:00
abstract：:On-line size-exclusion chromatography monitoring of potato phosphorylase catalyzed amylose synthesis--starting from alpha-D-glucose-1-P and maltohexaose--revealed rather monodisperse amylose populations. In the presence of lipids, amylose-lipid complexes spontaneously formed and precipitated. They were recovered by ce...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0502011
更新日期：2005-09-01 00:00:00
abstract：:Thermoresponsive polymers with lower critical solution temperatures (LCSTs) are of significant interest for a wide range of applications from sensors to drug delivery vehicles. However, the most widely investigated LCST polymers have nondegradable backbones, limiting their applications in vivo or in the environment. D...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00899
更新日期：2020-09-14 00:00:00
abstract：:Peptide RATEA16 spontaneously self-assembled into higher-order nanofiber hydrogels with extremely high water content (>99.5% (wt/vol)) under physiological condition. The hydrogels could undergo pH-reversible transitions from viscous solution to elastic hydrogel and to precipitate. The supramolecular self-assembly and ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701143g
更新日期：2008-06-01 00:00:00
abstract：:In this work, biodegradable branched polycationic polymers were synthesized by Michael addition polymerization from different amine monomers and the triacrylate monomer trimethylolpropane triacrylate. The polymers varied in the number of amines that dissociate in different pH ranges, which are considered to be benefic...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901147k
更新日期：2010-03-08 00:00:00
abstract：:The competing mechanisms that regulate adhesion of bacteria to surfaces and subsequent biofilm formation remain unclear, though nearly all studies have focused on the role of physical and chemical properties of the material surface. Given the large monetary and health costs of medical-device colonization and hospital-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701430y
更新日期：2008-06-01 00:00:00
abstract：:Aggregation phenomena in aqueous solutions of purified human tracheobronchial mucin have been studied by rheological methods, steady-state fluorescence, quasielastic light scattering, and spin probe techniques. At temperatures below 30 degrees C and concentrations above 15 mg/mL and in the absence of chaotropic agents...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm005532m
更新日期：2000-10-01 00:00:00
abstract：:Bionanocomposite materials, combining the properties of biopolymers and nanostructured materials, are attracting interest of the wider scientific community due to their potential application in design of implants, drug delivery systems, and tissue design platforms. Herein, we report on the use of maleimide-coated silv...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00101
更新日期：2015-04-13 00:00:00
abstract：:Glycogen is mainly found as the principal storage form of glucose in cells. Many bacteria are able to synthesize large amounts of glycogen under unfavorable life conditions. By combining infrared spectroscopy, single molecule force spectroscopy (SMFS) and immuno-staining technique, we evidenced that planktonic P. flu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300497c
更新日期：2012-07-09 00:00:00
abstract：:Micellar peroxidase-catalyzed synthesis of chiral polyaniline (PANI) in the presence of dodecylbenzenesulfonic acid (DBSA) was developed. The effect of DBSA concentration on the catalytic efficiency of horseradish and palm tree peroxidases was examined. Favorable conditions for the enzymatic synthesis of chiral PANI, ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070212p
更新日期：2007-08-01 00:00:00
abstract：:Amphiphilic ABC triblock copolymers composed of monomethoxy-capped poly(ethylene glycol) (MPEG), poly(2-(dimethylamino)ethyl methacrylate) (DMA), and poly(2-(diethylamino)ethyl methacrylate) (DEA) have been synthesized by atom transfer radical polymerization (ATRP). These copolymers dissolve molecularly in acidic aque...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030026t
更新日期：2003-11-01 00:00:00
abstract：:Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049941k
更新日期：2004-07-01 00:00:00
abstract：:Alditol bearing chitosans have shown the ability to reduce silver ions in mild conditions and without addition of exogenous reducing agents. The ion reduction induces the formation of a lactone moiety on the polysaccharide (Fetizon reaction) without causing C-C bond cleavage on the polyol. The close and multivalent ar...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801253c
更新日期：2009-02-09 00:00:00
abstract：:Structures of three pectic arabinogalactans, one from Vernonia kotschyana (Vk2a) and two from Cochlospermum tinctorium (Ct50A1 and Ct50A2), and their complement fixation and induction of B cell proliferation in vitro were compared. The polysaccharide Vk2a expressed potent biological activity in both assays compared wi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050355g
更新日期：2006-01-01 00:00:00
abstract：:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore electrospinning for protein-based bi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025581u
更新日期：2002-11-01 00:00:00
abstract：:We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700690p
更新日期：2007-11-01 00:00:00
abstract：:Regenerated silk fibroin (RSF) fibers were obtained by extruding a concentrated aqueous silk fibroin solution into an ammonium sulfate coagulation bath. A custom-made simplified industrial-type wet-spinning device with continuous mechanical postdraw was used. The effect of dope concentration, coagulation bath, extrusi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900840h
更新日期：2010-01-11 00:00:00
abstract：:There is a lack of hydrogel materials whose properties can be tuned at the point of use. Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds because of their low mechanical strength. Their mechanics can be improved through cross-linking and chem...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01251
更新日期：2016-03-14 00:00:00
abstract：:Minimally invasive implants and/or scaffolds integrated with multiple functionalities are of interest in the clinical settings. In this paper, chitosan (CTS) functionalized poly(lactic-co-glycolic acid) (PLGA) microspheres containing a model payload, lysozyme (Lyz), were prepared by a water-in-oil-in-water emulsion me...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm4003464
更新日期：2013-06-10 00:00:00
abstract：:Nanoparticles (NPs) with high drug loading and pH-responsivity were prepared by nanoprecipitation of a hydrophobic polymer-drug conjugate (PDC). The PDC, polylactide-graft-doxorubicin (PLA-g-DOX), was synthesized by azide-alkyne click reaction to transform acetylene-functionalized PLA into PLA-graft-aldehyde (PLA-g-AL...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401471p
更新日期：2014-02-10 00:00:00
