Abstract：
:The competing mechanisms that regulate adhesion of bacteria to surfaces and subsequent biofilm formation remain unclear, though nearly all studies have focused on the role of physical and chemical properties of the material surface. Given the large monetary and health costs of medical-device colonization and hospital-acquired infections due to bacteria, there is considerable interest in better understanding of material properties that can limit bacterial adhesion and viability. Here we employ weak polyelectrolyte multilayer (PEM) thin films comprised of poly(allylamine) hydrochloride (PAH) and poly(acrylic acid) (PAA), assembled over a range of conditions, to explore the physicochemical and mechanical characteristics of material surfaces controlling adhesion of Staphylococcus epidermidis bacteria and subsequent colony growth. Although it is increasingly appreciated that eukaryotic cells possess subcellular structures and biomolecular pathways to sense and respond to local chemomechanical environments, much less is known about mechanoselective adhesion of prokaryotes such as these bacteria. We find that adhesion of viable S. epidermidis correlates positively with the stiffness of these polymeric substrata, independently of the roughness, interaction energy, and charge density of these materials. Quantitatively similar trends observed for wild-type and actin analogue mutant Escherichia coli suggest that these results are not confined to only specific bacterial strains, shapes, or cell envelope types. These results indicate the plausibility of mechanoselective adhesion mechanisms in prokaryotes and suggest that mechanical stiffness of substrata materials represents an additional parameter that can regulate adhesion of and subsequent colonization by viable bacteria.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Lichter JA,Thompson MT,Delgadillo M,Nishikawa T,Rubner MF,Van Vliet KJdoi
10.1021/bm701430ysubject
Has Abstractpub_date
2008-06-01 00:00:00pages
1571-8issue
6eissn
1525-7797issn
1526-4602journal_volume
9pub_type
杂志文章abstract：:The copolymer of methyl methacrylate (MMA) and 2-[2',3',5'-triiodobenzoyl]oxoethyl methacrylate (1), ratio 3:1 (mass:mass), was prepared via a free-radical polymerization in bulk. The copolymer (M(w) = 97.8 kD and M(n) = 41.5 kD) was dissolved in chloroform and subsequently transformed into beads with a diameter in th...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030002m
更新日期：2003-05-01 00:00:00
abstract：:In this work we describe the application of amphiphilic N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymers as polymeric surfactants in miniemulsion techniques. HPMA-based copolymers with different ratios of HPMA (hydrophilic) to laurylmethacrylate (LMA; hydrophobic) units were synthesized by RAFT polymerization...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301453g
更新日期：2012-12-10 00:00:00
abstract：:The development of polymers that are both bactericidal and biocompatible would have many applications and are currently of research interest. Following the development of strongly bactericidal copolymers of 4-vinylpyridine and poly(ethylene glycol) methyl ether methacrylate, biocompatibility assays have been completed...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1000839
更新日期：2010-05-10 00:00:00
abstract：:Evidence is presented that the polysaccharide rhamnogalacturonan I (RGI) can be biosynthesized in remarkably organized branched configurations and surprisingly long versions and can self-assemble into a plethora of structures. AFM imaging has been applied to study the outer mucilage obtained from wild-type (WT) and mu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01756
更新日期：2020-04-13 00:00:00
abstract：:The interaction between positively charged poly(amido amine) (PAMAM) dendrimers of generation 4 and DNA has been investigated for two DNA lengths; 2000 basepairs (bp; L = 680 nm) and 4331 bp (L = 1472.5 nm) using a theoretical model by Schiessel for a semiflexible polyelectrolyte and hard spheres. The model was modifi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9000662
更新日期：2009-07-13 00:00:00
abstract：:Silicatein α exists within the protein filament of silica spicules of the marine sponge Tethya aurantium in a predominantly β-sheet structure. However, it is produced in a soluble form with mixed α-helix/β-sheet structure akin to its cathepsin L homologue. To understand this conformational transition in the context of...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100932e
更新日期：2010-11-08 00:00:00
abstract：:Developing green and nontoxic biomaterials, derived from renewable sources and processable through 3D bioprinting technologies, is an emerging challenge of sustainable tissue engineering. Here, pectin from citrus peels was cross-linked for the first time with (3-glycidyloxypropyl)trimethoxysilane (GPTMS) through a one...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01332
更新日期：2020-02-10 00:00:00
abstract：:Inulin, the polydisperse reserve polysaccharide from chicory, has been modified by carbamoylation in organic solvents. The reaction of inulin with a range of alkyl isocyanates resulted, after crystalization, in a variety of carbamoylated inulins from which the interfacial properties were determined. The medium and lon...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015570l
更新日期：2001-01-01 00:00:00
abstract：:This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800234r
更新日期：2008-09-01 00:00:00
abstract：:We have designed α-helical peptides de novo that can induce aggregation of various kinds of cells by focusing on physicochemical properties such as hydrophobicity, net charges, and amphipathicity. It is shown that peptide hydrophobicity is the key factor to determine capabilities for cell aggregation while peptide net...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm4014414
更新日期：2014-02-10 00:00:00
abstract：:Algae constitute a largely available, low value material from renewable resources of marine origin to be used for the production of eco-compatible composites. Fibers of the green alga Ulva armoricana from the French coast were positively evaluated for the production of composites with a hydrophilic, eco-compatible pol...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701041e
更新日期：2008-03-01 00:00:00
abstract：:Cationic kraft lignin (CKL) macromolecules were produced via polymerizing kraft lignin (KL) with [2-(acryloyloxy)ethyl]trimethylammonium chloride (ATAC) or [2-(methacryloyloxy)ethyl]trimethylammonium methyl sulfate (METAM). Despite slightly different charge densities (2.3-2.5 mmol/g) of CKL, lignin-METAM (KL-METAM) ha...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01016
更新日期：2019-10-14 00:00:00
abstract：:Optimal curcumin delivery for medicinal applications requires a drug delivery system that both solubilizes curcumin and prevents degradation. To achieve this, curcumin has been encapsulated in submicrometer chitosan/Tween 20 particles via a benchtop spray-drying process. Spray-drying parameters have been optimized usi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300564v
更新日期：2012-08-13 00:00:00
abstract：:Since their discovery at the end of the previous millennium, carbon nanotubes (CNTs) have been the object of thousands of papers describing their applications in fields ranging from physics to electronics, photonics, chemistry, biology, and medicine. The development of chemical approaches to modify their graphitic sid...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm201020h
更新日期：2011-10-10 00:00:00
abstract：:The influence of 2,2,6,6-tetrametylpiperidine-1-oxyl (TEMPO)-mediated oxidation of wood cellulose and the mechanical disintegration of oxidized cellulose in water on degree of polymerization determined by viscosity measurement (DP(v)) and the apparent length of the TEMPO-oxidized cellulose nanofibrils (TOCNs) was inve...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2017542
更新日期：2012-03-12 00:00:00
abstract：:Biomaterials conceived for vectorization of bioactives are currently considered for biomedical, biological, and environmental applications. We have produced a pH-sensitive biomaterial composed of natural source alginate and chitosan polysaccharides for application as a drug delivery system via oral administration. The...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01630
更新日期：2018-02-12 00:00:00
abstract：:N-(3-Aminopropyl)pyrrole was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a pyrrole-alginate conjugate for subsequent use in biosensor applications. The pyrrole-alginate conjugate was quantified by UV spectroscopy at 230 nm, by an HPSEC...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050339j
更新日期：2005-11-01 00:00:00
abstract：:Tiopronin (N-(2-mercaptopropionyl)glycine)-protected gold nanoparticles (TPAu) were cross-linked to collagen via EDC (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide) coupling. On average, each TPAu forms eight amide bonds with collagen lysine moieties. The resulting gels were studied with environmental SEM, TEM, micr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800793z
更新日期：2008-12-01 00:00:00
abstract：:We report that the continuous assembly of polymers (CAP) approach, mediated by ring-opening metathesis polymerization (ROMP), is a facile and versatile technology to prepare engineered nanocoatings for various biomedical applications. Low-fouling coatings on particles were obtained by the formation of multicomposition...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400680d
更新日期：2013-08-12 00:00:00
abstract：:Methylprednisolone steroid esters of hyaluronan differing in degree of functionalization and molecular weight were investigated in aqueous solution. Conformation and aggregation phenomena were elucidated by means of circular dichroism, viscometry, rheology, and nuclear magnetic resonance, mainly by (1)H pulsed field g...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049221d
更新日期：2005-05-01 00:00:00
abstract：:Enhancing the molecular orientation of the regenerated silk fibroin (RF) up to a level comparable to the native silk is highly challenging. Our novel and promising strategy for the poststretching process is (1) creating at first an α-helix-HFIP complex with a hexagonal packing as an intermediate state and then (2) str...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00043
更新日期：2016-04-11 00:00:00
abstract：:The aggregation of meso-tetra(4-sulfonatophenyl)porphyrin (H(2)TPPS(4-)) in phosphate solutions was investigated as a function of pH, concentration, time, ionic strength, and solution preparation (either from dilution of a freshly prepared 2 mM stock or by direct preparation of μM solution concentrations) using a comb...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201078d
更新日期：2012-01-09 00:00:00
abstract：:Cellulose nanocrystals (CNCs) are mechanically rigid, toxicologically benign, fiber-like nanoparticles. They can easily be extracted from renewable biosources and have attracted significant interest as reinforcing fillers in polymers. We here report the modification of CNCs with the 2-ureido-4[1H]pyrimidinone (UPy) mo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01639
更新日期：2017-02-13 00:00:00
abstract：:The shear rheology of a microcrystalline cellulose dissolved in a 9% NaOH aqueous solution was studied in the steady and oscillatory modes. The cellulose-(9% NaOH-H(2)O) mixtures show not to be true solutions. In the dilute regime, with cellulose concentration below 1%, the rheological behavior is typical of the one o...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm020100s
更新日期：2003-03-01 00:00:00
abstract：:It was aim of the study to investigate the in vivo potential of a novel insulin-thiomer complex nanoparticulate delivery system. Insulin loaded nanoparticles were obtained by the formation of hydrogen bonds between poly(vinyl pyrrolidone) (PVP) and poly(acrylic acid)-cysteine (PAA-Cys) or poly(acrylic acid) (PAA), res...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700916h
更新日期：2008-01-01 00:00:00
abstract：:Electrospinning is a simple and versatile technique of producing polymeric fibers ranging from submicron to micron in diameter. Incorporation of bioactive agents into the fibers could make a biofunctional tissue engineering scaffold. In this study, we investigated the feasibility of encapsulating human beta-nerve grow...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0501149
更新日期：2005-07-01 00:00:00
abstract：:Micellar peroxidase-catalyzed synthesis of chiral polyaniline (PANI) in the presence of dodecylbenzenesulfonic acid (DBSA) was developed. The effect of DBSA concentration on the catalytic efficiency of horseradish and palm tree peroxidases was examined. Favorable conditions for the enzymatic synthesis of chiral PANI, ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070212p
更新日期：2007-08-01 00:00:00
abstract：:Copolymers of (R)-3-hydroxybutyric acid (HB) and epsilon-caprolactone (CL) with a composition ranging from 28 to 81 mol % of HB were synthesized by transesterification of the corresponding homopolymers in solution in the presence of 4-toluenesulfonic acid. The copolyesters were characterized with regard to their molec...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025525t
更新日期：2002-07-01 00:00:00
abstract：:pH-dependent release from monoolein (MO) cubic phase was obtained by taking advantage of complex coacervation between hydrophobically modified alginate (HmAL) and hydrophobically modified silk fibroin (HmSF) in the water channels. The degree of coacervation was investigated at pH 3.0 by a light scattering method and t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm101249e
更新日期：2011-02-14 00:00:00
abstract：:Inorganic polyphosphate (polyP) released by human platelets has recently been shown to activate blood clotting and identified as a potential target for the development of novel antithrombotics. Recent studies have shown that polymers with cationic binding groups (CBGs) inhibit polyP and attenuate thrombosis. However, ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00327
更新日期：2018-04-09 00:00:00
