Abstract:
:Methylprednisolone steroid esters of hyaluronan differing in degree of functionalization and molecular weight were investigated in aqueous solution. Conformation and aggregation phenomena were elucidated by means of circular dichroism, viscometry, rheology, and nuclear magnetic resonance, mainly by (1)H pulsed field gradient (PFG) NMR, which allows the determination of the diffusion coefficient of the species under investigation. The functionalization of hyaluronan with the steroid induces a reduction of the molecular volume, as a consequence of intramolecular hydrophobic interactions. For concentrated samples we have observed the coexistence of unimolecular collapsed chains and of aggregates, the latter disappearing upon dilution. The methylprednisolone ester of lower molecular weight hyaluronan has a larger molecular volume than its higher molecular weight analogue, even though still smaller than the underivatized polymer. This effect can be explained with the reduced flexibility of the polymer backbone probably impairing intramolecular interactions.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Taglienti A,Valentini M,Sequi P,Crescenzi Vdoi
10.1021/bm049221dkeywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
1648-53issue
3eissn
1525-7797issn
1526-4602journal_volume
6pub_type
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