Abstract：
:A major challenge in drug delivery is the internalization through the apical plasma membrane of the polarized epithelial cells lining organs facing the external environment, e.g., lungs and the gastrointestinal tract. The reduced permeation of drugs entering through this pathway is in part due to the mucosal barrier and low rate of endocytosis at these membranes. We investigated the possible role of nanoparticle surface charge on its entry through the apical plasma membrane and its intracellular pathway. We found that both cationic and anionic nanoparticles are targeted mainly to the clathrin endocytic machinery. A fraction of both nanoparticle formulations is suspected to internalize through a macropinocytosis-dependent pathway. A significant amount of nanoparticles transcytose and accumulate at the basolateral membrane. Some anionic but not cationic nanoparticles transited through the degradative lysosomal pathway. Taken together, these observations indicate that cationic nanoparticles, in addition to their potential for drug delivery to epithelia, may be promising carriers for transcytosing drugs to the blood stream.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Harush-Frenkel O,Rozentur E,Benita S,Altschuler Ydoi
10.1021/bm700535psubject
Has Abstractpub_date
2008-02-01 00:00:00pages
435-43issue
2eissn
1525-7797issn
1526-4602journal_volume
9pub_type
杂志文章abstract：:We have investigated the thermodynamic and dynamic behavior of multistranded β-lactoglobulin protein fibrils in water, by combining static, dynamic, and depolarized dynamic light scattering (SLS, DLS, DDLS), small angle neutron scattering (SANS), rheology, and cryogenic transmission electron microscopy (cryo-TEM). We ...
journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2012-10-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2000-10-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2008-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3007117
更新日期：2012-09-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700541e
更新日期：2007-10-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00508
更新日期：2016-09-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301720g
更新日期：2013-02-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400206m
更新日期：2013-04-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01063
更新日期：2017-12-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01175
更新日期：2020-02-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3003286
更新日期：2012-06-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01939
更新日期：2017-03-13 00:00:00
abstract：:The islet amyloid polypeptide (IAPP) is a regulatory peptide that can aggregate into fibrillar structures associated with type 2 diabetes. In this study, the IAPP21-27 segment was modified with a biotin linker at the N-terminus (Btn-GNNFGAIL) to immobilize peptide fibrils on streptavidin-coated surfaces. Key residues ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01466
更新日期：2020-02-10 00:00:00
abstract：:Trimethylsilylation of chitin was studied in detail to establish a reliable method, and the properties of the resulting product were elucidated. Chitin was successfully trimethylsilylated with a mixture of hexamethyldisilazane and trimethylsilyl chloride in pyridine. Compared to alpha-chitin, beta-chitin was much more...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049295p
更新日期：2005-05-01 00:00:00
abstract：:We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe3O4 nanoparticles that were synthesized using the (Fe3+/Fe2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupli...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01778
更新日期：2017-03-13 00:00:00
abstract：:A N,N-dimethylacrylamide-based hydrogel (2) with the new cross-linker (ethylenedioxy) bis[2,2'-(N-acryloylamino)ethane] (1) has been prepared, and its physicochemical properties in aqueous solution were studied. Three different native proteins (lysozyme, bovine serum albumin, and rabbit IgG) were encapsulated within t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm010147y
更新日期：2002-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm801103c
更新日期：2009-01-12 00:00:00
abstract：:Silkworm silk has excellent mechanical properties, biocompatibility, and promising applications in the biomedical sector. Silkworms spin silk at the beginning and end of each of their five instar stages, as well as spinning mature silk after the fifth instar. We evaluated the mechanical properties and structure of 10 ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01576
更新日期：2019-03-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01313
更新日期：2017-12-11 00:00:00
abstract：:Core-shell structured nanoparticles (NPs) render the simultaneous coloading capacity of both hydrophobic and hydrophilic drugs and may eventually enhance therapeutic efficacy. In this study, we employed a facile squalenoylation technology to synthesize a new amphiphilic starch derivative from partially oxidized starch...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01430
更新日期：2020-12-21 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300523e
更新日期：2012-06-11 00:00:00
abstract：:Utilization of reversible interactions as sacrificial bonds in biopolymers is critical for the integral synthesis of mechanically superior biological materials. In this work, cellulose nanofibrils (CNFs) reinforced covalent polyacrylamide (PAAm) composite hydrogels are immersed into multivalent cation (Ca2+, Zn2+, Al3...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01915
更新日期：2017-03-13 00:00:00
abstract：:Poly((N-vinylpyrrolidone)-block-poly(vinyl acetate)) (PVP-b-PVAc) block copolymers of varying molecular weight and hydrophobic block lengths were synthesized via controlled radical polymerization and investigated as carriers for the solubilization of highly hydrophobic riminophenazine compounds. These compounds have r...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301410d
更新日期：2012-12-10 00:00:00
abstract：:Spider dragline silk exhibits an extraordinary toughness and is typically composed of two types of major ampullate spidroins (MaSp1 and MaSp2), differing in their proline content and hydrophobicity. In this paper, we recombinantly produced an unusual but naturally occurring short major ampullate spidroin (MaSp1s) as a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00090
更新日期：2017-04-10 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050086u
更新日期：2005-07-01 00:00:00
abstract：:Cellulose is the most familiar and most abundant strong biopolymer, but the reasons for its outstanding mechanical performance are not well understood. Each glucose unit in a cellulose chain is joined to the next by a covalent C-O-C linkage flanked by two hydrogen bonds. This geometry suggests some form of cooperativi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401616n
更新日期：2014-03-10 00:00:00
abstract：:Microgels based on poly(vinyl alcohol), PVA, grafted with methacrylate side chains, MA, incorporating N-isopropylacrylamide, NiPAAm, monomer, were prepared by water-in-water emulsion polymerization method. These systems exhibit a spherical shape and a volume-phase transition, that is, shrinking, below physiological te...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900185u
更新日期：2009-06-08 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01278
更新日期：2018-11-12 00:00:00
abstract：:Tannic acid (TA) can form stable complexes with proteins, attracting significant attention as protein delivery systems. However, its systemic application has been limited due to nonspecific interaction. Here, we report a simple technique to prepare systemically applicable protein delivery systems using sequential self...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00903
更新日期：2020-09-14 00:00:00
abstract：:Amphiphilic ABC triblock copolymers composed of monomethoxy-capped poly(ethylene glycol) (MPEG), poly(2-(dimethylamino)ethyl methacrylate) (DMA), and poly(2-(diethylamino)ethyl methacrylate) (DEA) have been synthesized by atom transfer radical polymerization (ATRP). These copolymers dissolve molecularly in acidic aque...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm030026t
更新日期：2003-11-01 00:00:00
