Abstract:
:Strong signaling cascades derived from upregulation and overexpression of growth factors such as the EGF-family (epidermal growth factors) have been crucially related to cancer pathogenesis. Gene silencing techniques to modulate the expression of oncogenes and tumor suppresor genes are a strategy that shows great promise for cancer management but still faces some limitations in the design of biocompatible and effective vectors. In this study, we synthesized, by reversible addition-fragmentation chain transfer (RAFT) polymerization, several acid degradable galactose-based hyperbranched cationic polymers with varying molecular weights (10 to 20 kDa) and compositions with 2-lactobioamidoethyl methacrylamide [LAEMA] and 2-aminoethyl methacrylamide hydrochloride [AEMA] at different ratios (2.0, 1.0, and 0.5). These polymers were then evaluated for their ability to enhance Epidermal Growth Factor Receptor (EGFR) knockdown in cervical carcinoma. All the polymer constructs have enhanced capabilities to condensate siRNA (small interfering RNA), showing low toxicity at higher LAEMA:AEMA ratios (1.0 and 2.0). Western blot assays were conducted to quantify the EGFR expression of each treatment group demonstrating superior gene knockdown efficiency for the polymers having a LAEMA:AEMA ratio of 2.0 than the lower ratio counterparts; while maintaining low toxicity levels. Gene silencing of EGFR of up to 60% was achieved with acid degradable polymers having 10 kDa molecular weight and a LAEMA:AEMA ratio of 2.0. The superior stability of the polyplexes under physiological conditions and the low cytotoxicity observed in the 48 h post-transfection demonstrated the high potential of these acid degradable galactose-based hyperbranched cationic polymers for EGFR silencing treatment applications at the clinical level.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Peng YY,Diaz-Dussan D,Kumar P,Narain Rdoi
10.1021/acs.biomac.8b01066subject
Has Abstractpub_date
2018-10-08 00:00:00pages
4052-4058issue
10eissn
1525-7797issn
1526-4602journal_volume
19pub_type
杂志文章abstract::A hyperbranched conjugated polyelectrolyte (HCPE) with a core-shell structure is designed and synthesized via alkyne polycyclotrimerization and click chemistry. The HCPE has an emission maximum at 565 nm with a quantum yield of 12% and a large Stokes shift of 143 nm in water. By virtue of its poly(ethylene glycol) she...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200563a
更新日期:2011-08-08 00:00:00
abstract::Many biological processes are regulated by reversible binding events, with these interactions between macromolecules representing the core of dynamic chemistry. As such, any attempt to gain a better understanding of such interactions, which would pave the way to the extrapolation of natural designs to create new advan...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01103
更新日期:2015-10-12 00:00:00
abstract::The electrospinning of polymer melts can offer an advantage over solution electrospinning, in the development of layered tissue constructs for tissue engineering. Melt electrospinning does not require a solvent, of which many are cytotoxic in nature, and the use of nonwater soluble polymers allows the collection of fi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050777q
更新日期:2006-03-01 00:00:00
abstract::Nanoparticles with high aspect ratios have favorable attributes for drug delivery and bioimaging applications based on their enhanced tissue penetration and tumor homing properties. Here, we investigated a novel filamentous viral nanoparticle (VNP) based on the Pepino mosaic virus (PepMV), a relative of the establishe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01365
更新日期:2019-01-14 00:00:00
abstract::This work reports on the effect of CO2 at subcritical conditions and the gaseous state on the telechelic poly(epsilon-caprolactone) polymers. The tested polymers are semi-crystalline in nature and thus the effect of functional groups and their overall contribution to foaming and formation of microstructures with open-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050223n
更新日期:2005-09-01 00:00:00
abstract::One of the major drawbacks found in most bone tissue engineering approaches developed so far consists in the lack of strategies to promote vascularisation. Some studies have addressed different issues that may enhance vascularisation in tissue engineered constructs, most of them involving the use of growth factors (GF...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8014973
更新日期:2009-06-08 00:00:00
abstract::In this work, biodegradable branched polycationic polymers were synthesized by Michael addition polymerization from different amine monomers and the triacrylate monomer trimethylolpropane triacrylate. The polymers varied in the number of amines that dissociate in different pH ranges, which are considered to be benefic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901147k
更新日期:2010-03-08 00:00:00
abstract::The shear rheology of a microcrystalline cellulose dissolved in a 9% NaOH aqueous solution was studied in the steady and oscillatory modes. The cellulose-(9% NaOH-H(2)O) mixtures show not to be true solutions. In the dilute regime, with cellulose concentration below 1%, the rheological behavior is typical of the one o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm020100s
更新日期:2003-03-01 00:00:00
abstract::The formation of a suitable extracellular matrix (ECM) that promotes cell adhesion, organization, and proliferation is essential within biomaterial scaffolds for tissue engineering applications. In this work, short elastin mimetic peptide sequences, EM-19 and EM-23, were engineered to mimic the active motifs of human ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101214f
更新日期:2011-02-14 00:00:00
abstract::Dragline silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301110s
更新日期:2012-12-10 00:00:00
abstract::Hydrogels constructed from folded protein domains are of increasing interest as resilient and responsive biomaterials, but their optimization for applications requires time-consuming and costly molecular design. Here, we explore a complementary approach to control their properties by examining the influence of crossli...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01044
更新日期:2020-10-12 00:00:00
abstract::Block copolypeptides, which combine the self-assembly of block copolymers and the highly ordered 3D structures of proteins, are potential candidates for novel supramolecular structures and biotech applications, such as biosensors, tissue engineering, and selective drug delivery. The synthesis of model block copolypept...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0497217
更新日期:2004-09-01 00:00:00
abstract::The time-dependent diffusion and mechanical properties of gelatin in solution, in the gel state, and during the sol/gel transition were determined using fluorescence recovery after photobleaching (FRAP) and rheology. The parameters in the experimental design were 2% w/w and 5% w/w gelatin concentration; 15, 20, and 25...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1008487
更新日期:2010-12-13 00:00:00
abstract::We report the rational design of a chitosan-based drug delivery system. The chitosan derivative N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride (HTCC) was ionically cross-linked by sodium tripolyphosphate (TPP) to form sub-200-nm microgels that are responsive to pH changes. When these microgels were loaded ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050912z
更新日期:2006-05-01 00:00:00
abstract::The massive use of antibiotics in healthcare and agriculture has led to their artificial accumulation in natural habitats, which risks the structure and function of the microbial communities in ecosystems, threatens food and water security, and accelerates the development of resistome. Ideally, antibiotics should rema...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00163
更新日期:2020-06-08 00:00:00
abstract::Low molecular weight random copolymers bearing protonated primary amine groups and hydrophobic alkyl groups in the side chains were synthesized and their activities against E. coli , S. aureus , human red blood cells, and human epithelial carcinoma cells (HEp-2) were quantified. The mole fraction of alkyl side chains ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900784x
更新日期:2009-11-09 00:00:00
abstract::Tethering drug substances to a gel network is an effective way of controlling the release kinetics of hydrogel-based drug delivery systems. Here, we report on in situ forming, biodegradable hydrogels that allow for the covalent attachment of peptides or proteins. Hydrogels were prepared by step-growth polymerization o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901235g
更新日期:2010-02-08 00:00:00
abstract::We have constructed a tandem gene expression cassette containing three Ralstonia eutropha poly[(R)-3-hydroxybutyrate] (PHB) synthesis genes under the control of the Pichia pastoris glyceraldehyde-3-phosphate promoter and the green fluorescent protein (Gfp) under the control of the P. pastoris alcohol oxidase promoter....
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0494141
更新日期:2005-03-01 00:00:00
abstract::Poloxamer thermoresponsive gels are widely explored in controlled drug delivery. Nevertheless, these gels possess inadequate mechanical properties, poor bioadhesiveness, and high permeability to water. To overcome these issues, we blended mucoadhesive hyaluronic acid (HA) with poloxamer analogs. This study aimed to in...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1008958
更新日期:2011-01-10 00:00:00
abstract::Electrospun poly(glycolide-co-lactide) (PLA10GA90, LA/GA ratio 10/90) biodegradable nanofiber membranes possessed very high surface area to volume ratios and were completely noncrystalline with a relatively lowered glass transition temperature. These characteristics led to very different structure, morphology, and pro...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025717o
更新日期:2003-03-01 00:00:00
abstract::Native cellulose consists of a set of parallel chains composed of glucose. Most of the time, these chains are highly ordered and form a structure that is known as a microfibril. On the other hand, highly crystalline forms of cellulose are more difficult to process and often are unpredictable in their behavior. If an o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0101318
更新日期:2001-01-01 00:00:00
abstract::Dynamic mechanical properties of aqueous solutions of hydroxypropyl methyl cellulose (HPMC) were investigated using oscillatory shear measurements. The structure was investigated with light scattering. A systematic investigation of the frequency dependence of the shear moduli showed that HPMC forms two distinct interp...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401554s
更新日期:2014-01-13 00:00:00
abstract::Weissella confusa VTT E-90392 is an efficient producer of a dextran that is mainly composed of α-(1→6)-linked D-glucosyl units and very few α-(1→3) branch linkages. A mixture of the Chaetomium erraticum endodextranase and the Aspergillus niger α-glucosidase was used to hydrolyze W. confusa dextran to glucose and a set...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1011536
更新日期:2011-02-14 00:00:00
abstract::Amino acid-based poly(ester urea) (PEU) copolymers functionalized with pendant catechol groups that address the need for strongly adhesive yet degradable biomaterials have been developed. Lap-shear tests with aluminum adherends demonstrated that these polymers have lap-shear adhesion strengths near 1 MPa. An increase ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501456g
更新日期:2015-01-12 00:00:00
abstract::Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. He...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00823
更新日期:2015-09-14 00:00:00
abstract::Major ampullate silk, also known as dragline silk, is one of the strongest biomaterials known. This silk is composed of two proteins, major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2). Only partial cDNA sequences have been obtained for these proteins, and these sequences are toward the C-termin...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050472b
更新日期:2005-11-01 00:00:00
abstract::It is an important challenge for bone repair to effectively deliver growth factors and at the same time to prevent and cure inflammation without obvious pathogen resistance. We designed a kind of antibacterial peptide-mimetic alternating copolymers (PMACs) to effectively inhibit and kill both Gram-positive and Gram-ne...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01209
更新日期:2017-12-11 00:00:00
abstract::This work demonstrates a versatile and environmentally friendly route for the development of new orthogonal monomers that can be used for postfunctionalizable polymer networks. A monomer containing both vinyl ether (VE) and cyclic disulfide moieties was synthesized via enzyme catalysis under benign reaction conditions...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01710
更新日期:2019-03-11 00:00:00
abstract::Reductively degradable biopolymers have emerged as a unique class of smart biomedical materials. Here, a functional coupling agent, cystamine diisocyanate (CDI), was designed to offer a facile access to reductively degradable biopolymers via polycondensation with various diols. CDI was readily obtained with a decent y...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01578
更新日期:2016-03-14 00:00:00
abstract::Fluorescence and circular dichroism spectroscopy were used to monitor the conformational transition of regenerated Bombyx mori silk fibroin (RSF) in aqueous solutions under different conditions. According to the analysis of fluorescence spectra using anilinonaphthalene-8-sulfonic acid magnesium salt (ANS) as an extern...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0343848
更新日期:2004-05-01 00:00:00