Abstract：
:Dragline silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipes and A. aurantia , indicates that MaSp1 proteins are more easily formed into β-sheet nanostructures, while MaSp2 proteins form random coil and helical structures. To test if this behavior of natural silk proteins could be reproduced by recombinantly produced spider silk mimic protein, recombinant MaSp1/MaSp2 mixed fibers as well as chimeric silk fibers from MaSp1 and MaSp2 sequences in a single protein were produced based on the variable ratio and conserved motifs of MaSp1 and MaSp2 in native silk fiber. Mechanical properties, solid-state NMR, and XRD results of tested synthetic fibers indicate the differing roles of MaSp1 and MaSp2 in the fiber and verify the importance of postspin stretching treatment in helping the fiber to form the proper spatial structure.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
An B,Jenkins JE,Sampath S,Holland GP,Hinman M,Yarger JL,Lewis Rdoi
10.1021/bm301110ssubject
Has Abstractpub_date
2012-12-10 00:00:00pages
3938-48issue
12eissn
1525-7797issn
1526-4602journal_volume
13pub_type
杂志文章abstract：:New therapeutic strategies for the treatment of neoplastic pathologies and, in particular, metastasis processes are based on the inhibitory effect of angiogenic processes. The present article deals with the design, preparation, and application of new "polymer drugs" with a clear inhibitory effect of the activation of ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100223d
更新日期：2010-07-12 00:00:00
abstract：:A series of Vitamin E (vitE)-labeled PEIs (PEI-vitEn) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitEn/pDNA complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell line...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00776
更新日期：2016-10-10 00:00:00
abstract：:The drying dynamics of protein coatings is of importance for many applications. The main focus of research so far was to investigate macroscopic properties of protein coatings, leaving drying dynamics virtually unexplored. A unique combination of techniques is used to monitor drying of a coating containing the protein...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501549b
更新日期：2015-01-12 00:00:00
abstract：:Tumorigenic cell behaviors can be suppressed or enhanced by their physicochemical environment. As a first step toward developing materials that allow tumorigenic behaviors to be observed and manipulated, we cultured related MCF10 breast cell lines on fibers composed of the Drosophila protein Ultrabithorax (Ubx). These...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01311
更新日期：2016-11-14 00:00:00
abstract：:Thin films of a biocompatible and nonbiofouling poly(oligo(ethylene glycol) methacrylate) ( pOEGMA) with various thicknesses were formed on gold and Si/SiO 2 substrates by a combination of the formation of self-assembled monolayers (SAMs) terminating in bromoester-an initiator of atom transfer radical polymerization (...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm7009043
更新日期：2007-12-01 00:00:00
abstract：:We have designed α-helical peptides de novo that can induce aggregation of various kinds of cells by focusing on physicochemical properties such as hydrophobicity, net charges, and amphipathicity. It is shown that peptide hydrophobicity is the key factor to determine capabilities for cell aggregation while peptide net...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm4014414
更新日期：2014-02-10 00:00:00
abstract：:The barrier functions of the stratum corneum and the epidermal layers present a tremendous challenge in achieving effective transdermal delivery of drug molecules. Although a few reports have shown that poly(amidoamine) (PAMAM) dendrimers are effective skin-penetration enhancers, little is known regarding the fundamen...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300545b
更新日期：2012-07-09 00:00:00
abstract：:We studied methoxy poly(ethylene glycol)-b-poly(ε-caprolactone)-b-poly(2-dimethylaminoethyl methacrylate) (mPEG-b-PCL-b-PDMAEMA) nanoparticles as the codelivery vector of hydrophobic drug and pDNA by employing dynamic light scattering (DLS), ζ potential, transmission electron microscopy (TEM), gel retardation assay, a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100410m
更新日期：2010-09-13 00:00:00
abstract：:Two linear alpha-(1,4)-D-glucans substrates, of degrees of polymerization DP approximately 150 and 6000, were exposed to maize starch-branching enzyme IIa (mSBEIIa) in vitro. The resulting branched alpha-glucans and their constituent chains (obtained by debranching) were analyzed by nuclear magnetic resonance (NMR) an...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701213p
更新日期：2008-03-01 00:00:00
abstract：:Self-assembled hydrogen-bonded networks of the polysaccharide pectin, a mechanically functional component of plant cell walls, have been of recent interest as biomimetic exemplars of physical gels, and the microrheological and strain-stiffening behaviors have been previously investigated. Despite this detailed rheolog...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00870
更新日期：2015-10-12 00:00:00
abstract：:To develop a safe, effective, and biocompatible gene delivery vector, a series of hydrophobic amino acid grafted chitosan (AGC) derivatives were synthesized by carbodiimide mediated coupling reaction. Chemical characterization of AGC polymers was performed by NMR and elemental analysis. AGC polymers demonstrated excel...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301720g
更新日期：2013-02-11 00:00:00
abstract：:Stereocomplexation of poly(L-lactide) (PLLA) with star shaped D-lactic acid (D-LA) oligomers with different architectures and end-groups clearly altered the degradation rate and affected the degradation product patterns. Altogether, nine materials were studied: standard PLLA and eight blends of PLLA with either 30 or ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300196h
更新日期：2012-04-09 00:00:00
abstract：:Bioresponsive polymers can enable the development of more effective drug delivery vehicles and medical materials. Acetal-modified polysaccharides allow pH-triggered solubility switching in a versatile and effective manner, but prior work has been limited to a combination of acyclic alkoxyisopropyl and cyclic isopropyl...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00215
更新日期：2019-05-13 00:00:00
abstract：:The polymerization of N-isopropylacrylamide (NIPAAm) with ionizable monomers results in pH-responsive lower critical solution temperature (LCST) polymer which works in an ionization-dependent manner. However, gradual ionization of the comonomer occurs at a broad pH range due to the electrostatic field generated by the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01465
更新日期：2019-04-08 00:00:00
abstract：:Cancer cells have a large amount of ROS (reactive oxygen species) because of disturbed ROS homeostasis. Cancer cells therefore undertake redox adaptation to drive proliferation in tumor environments and even survive during anticancer treatment by upregulating endogenous antioxidants. As one of antioxidant defense syst...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01802
更新日期：2019-02-11 00:00:00
abstract：:Electrospinning for the formation of nanoscale diameter fibers has been explored for high-performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In the present study we explore electrospinning for protein-based bi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025581u
更新日期：2002-11-01 00:00:00
abstract：:One of the major drawbacks found in most bone tissue engineering approaches developed so far consists in the lack of strategies to promote vascularisation. Some studies have addressed different issues that may enhance vascularisation in tissue engineered constructs, most of them involving the use of growth factors (GF...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8014973
更新日期：2009-06-08 00:00:00
abstract：:In the present study, we monitored the conformation and chemical stability of a hydrophilic bottlebrush (BB) polymer in pure water and buffered saline solutions. We correlated these parameters to lubricating and wear protecting properties. Using the surface forces apparatus (SFA), we show that the BB polymer partially...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01063
更新日期：2017-12-11 00:00:00
abstract：:The dynamics of silk protein in the presence and absence of water has been investigated by dielectric relaxation spectroscopy (DRS). The silk fibroin film cast from its water solution contains 4-7 wt % bound water molecules, which can be removed by dehydration at 165 °C. Temperature and frequency scans were performed ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1008316
更新日期：2010-10-11 00:00:00
abstract：:We synthesized extremely deformable red blood cell-like microgel particles and loaded them with bovine hemoglobin (Hb) to potentiate oxygen transport. With similar shape and size as red blood cells (RBCs), the particles were fabricated using the PRINT (particle replication in nonwetting templates) technique. Low cross...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm3007242
更新日期：2012-09-10 00:00:00
abstract：:This study shows the application of the electrospinning technique as a viable method for the encapsulation and stabilization of bifidobacterial strains. Poly(vinyl alcohol) (PVOH) was used as the encapsulating material because it is generally recognized as safe (GRAS), has a high oxygen barrier when dry, and is water ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900660b
更新日期：2009-10-12 00:00:00
abstract：:This study clarifies the contribution to overall osmotic kinetics of colloid osmotic pressure (Pi) and the interaction of synthetic colloids with the membrane. Solutions (6%) of dextran with weight average molecular weight (MW(w)) 68 800 (DEX 70), dextran with MW(w) 40 000 (DEX 40), hydroxyethyl starch with MW(w) 70 0...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0500143
更新日期：2005-05-01 00:00:00
abstract：:Marine mussels tether to seashore surfaces with byssal threads, proteinaceous fibers that effectively dissipate energy from crashing waves. Protein-metal coordination bonds have been proposed to contribute to the characteristic mechanical and self-healing properties of byssal threads; however, very little is understoo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00803
更新日期：2015-09-14 00:00:00
abstract：:In this work, cell-responsive polysaccharide hydrogels were prepared by a simple procedure based on the sequential bioconjugation and cross-linking of the polysaccharide backbone with bioactive peptides and poly(ethylene glycol)-bis(thiol) (PEG-(SH)2), respectively. Using thiol-ene reactions, we successfully functiona...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501613u
更新日期：2015-03-09 00:00:00
abstract：:Single-walled carbon nanotube (SWNT) is a pseudo-one-dimensional nanostructure capable of carrying/displaying a large number of bioactive molecules and species in aqueous solution. In this work, a series of dendritic beta-D-galactopyranosides and alpha-D-mannopyranosides with a terminal amino group were synthesized an...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800395e
更新日期：2008-09-01 00:00:00
abstract：:The interaction of iron with water-soluble polymer chitosan and monomer d-glucosamine is investigated by Mössbauer spectroscopy. The 4.2 K Mössbauer spectrum of Fe-water-soluble chitosan complex indicates the presence of a magnetic pattern and a quadrupole doublet, and analysis of the spectral data leads to the conclu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm020131n
更新日期：2003-05-01 00:00:00
abstract：:We have investigated the self-assembly of fluorenylmethoxycarbonyl-conjugated dialanine (Fmoc-AA) molecules using combined computational and experimental approaches. Fmoc-AA gels were characterized using transmission electron microscopy (TEM), circular dichroism (CD), Fourier transform infrared (FTIR), and wide-angle ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301007r
更新日期：2012-11-12 00:00:00
abstract：:Protein transduction domains (PTDs) that readily transverse cellular membranes are of great interest and are attractive tools for the intracellular delivery of bioactive molecules. Learning to program synthetic polymers and oligomers with the appropriate chemical information to capture adequately the biological activi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200694u
更新日期：2011-08-08 00:00:00
abstract：:Photoexcited TiO2 degrades biomolecules such as nucleic acids, cell membrane proteins, and enzymes. Stabilization of enzyme activity against the deactivation caused by the combination of TiO2-UV is essential if we are to develop novel hybrid materials exhibiting photocatalytic and biocatalytic activities useful for de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049728o
更新日期：2004-09-01 00:00:00
abstract：:A new family of cationic dendron-bearing lipids (CDLs) with poly(amidoamine) dendrons of first to third generation (named as A1, A2, and A3, respectively) was synthesized through a synthesis approach that permits facile variation of chemical structures. All CDLs could effectively bind small interfering RNA (siRNA) to ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm4011563
更新日期：2013-12-09 00:00:00
