Abstract：
:It is an important challenge for bone repair to effectively deliver growth factors and at the same time to prevent and cure inflammation without obvious pathogen resistance. We designed a kind of antibacterial peptide-mimetic alternating copolymers (PMACs) to effectively inhibit and kill both Gram-positive and Gram-negative bacteria. The minimum inhibition concentrations (MICs) of the PMACs against E. coli and S. aureus are 8.0 μg/mL, which are much lower than that of antibacterial peptides synthesized by other methods such as widely used ring-opening polymerization of N-carboxyanhydride. Furthermore, the PMACs can self-assemble into polymer vesicles (polymersomes) in pure water with low cytotoxicity (IC50 > 1000 μg/mL), which can encapsulate growth factors in aqueous solution and release them during long-term antibacterial process for facilitating bone repair. We also find that the alternating structure is essential for the excellent antibacterial activity. The in vivo tests in rabbits confirmed that the growth-factor-encapsulated antibacterial vesicles have better bone repair ability compared with control groups without antibacterial vesicles. Overall, we have provided a novel method for designing PMAC-based highly effective intrinsically antibacterial vesicles that may have promising biomedical applications in the future.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Zhou C,Yuan Y,Zhou P,Wang F,Hong Y,Wang N,Xu S,Du Jdoi
10.1021/acs.biomac.7b01209subject
Has Abstractpub_date
2017-12-11 00:00:00pages
4154-4162issue
12eissn
1525-7797issn
1526-4602journal_volume
18pub_type
杂志文章abstract：:Our previous studies have demonstrated the efficacy of generation 5 poly(amidoamine) dendrimers (G5-PAMAM) as a platform for the targeted delivery of chemotherapeutics. However, anticancer therapy can be subverted by anti-apoptotic changes in cancer cells. Bcl-2 and several of its peptides are commonly overexpressed i...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700727g
更新日期：2007-10-01 00:00:00
abstract：:Injectable in situ crosslinkable biomaterials seeded with multipotent progenitor cells and coupled with minimally invasive arthroscopic techniques are an attractive alternative for treating irregularly shaped osteochondral defects. An in situ crosslinkable poly(lactide-co-ethylene oxide-co-fumarate) (PLEOF) macromer h...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060671a
更新日期：2007-03-01 00:00:00
abstract：:In the present study, novel bionanocomposite materials with tunable properties were successfully prepared using a poly(lactic acid) (PLA) matrix and acetylated microfibrillated cellulose (MFC) as reinforcing agent. The acetylation of MFC was confirmed by FTIR and (13)C CP-MAS NMR spectroscopies. The grafting of acetyl...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901186u
更新日期：2010-02-08 00:00:00
abstract：:The intermolecular distributions of amide groups within two commercial LMA pectins was studied after removal of the methyl esters followed by fractionation of the different populations by anion exchange chromatography. The populations obtained had almost equal degrees of amidation while the values of the degree of blo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050960j
更新日期：2006-06-01 00:00:00
abstract：:Micellar peroxidase-catalyzed synthesis of chiral polyaniline (PANI) in the presence of dodecylbenzenesulfonic acid (DBSA) was developed. The effect of DBSA concentration on the catalytic efficiency of horseradish and palm tree peroxidases was examined. Favorable conditions for the enzymatic synthesis of chiral PANI, ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070212p
更新日期：2007-08-01 00:00:00
abstract：:Ruthenium(II) tris(bipyridine)-centered poly(ethylenimine) (Ru PEI) was synthesized via acid hydrolysis of Ru tris(bipyridine)-centered poly(2-ethyl-2-oxazoline) (Ru PEOX), and the luminescence, DNA entrapment, and transfection efficiencies were evaluated. Emission maxima for Ru PEI samples are red-shifted compared to...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700481h
更新日期：2007-09-01 00:00:00
abstract：:Gelatin microspheres are well-known for their capacity to release growth factors in a controlled manner, but gelatin microspheres do not calcify in the absence of so-called bioactive substances that induce deposition of calcium phosphate (CaP) bone mineral. This study has investigated if CaP nanocrystals can be incorp...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1006344
更新日期：2010-10-11 00:00:00
abstract：:Gastric mucin, a high molecular weight glycoprotein, is responsible for providing the gel-forming properties and protective function of the gastric mucus layer. Bulk rheology measurements in the linear viscoelastic regime show that gastric mucin undergoes a pH-dependent sol-gel transition from a viscoelastic solution ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0609691
更新日期：2007-05-01 00:00:00
abstract：:Enzymatic hydrolysis of selected copolymers of cellulose diacetate-graft-poly(L-lactide)s (CDA-g-PLLAs) were conducted with proteinase K for film specimens, which were solely quenched from the molten state or, further, annealed at temperatures below or above their glass transition temperatures. The hydrolysis rates de...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034453i
更新日期：2004-03-01 00:00:00
abstract：:A strategy to manipulate the disulfide bond breaking triggered unfolding, and subsequently assembly of human serum albumin (HSA) in a lipophilic drug-dependent manner is present. In this study, the hydrophobic region, a molecular switch of the HSA, was regulated to form HSA-paclitaxel (HSA-PTX) nanoparticles by a faci...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201401s
更新日期：2012-01-09 00:00:00
abstract：:RNA interference (RNAi) requires the intracellular delivery of RNA molecules to initiate the neutralization of targeted mRNA molecules, inhibiting the expression or translation of the targeted gene. Current polymers and lipids that are used to deliver RNA molecules are generally required to be positively charged, to a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01321
更新日期：2019-01-14 00:00:00
abstract：:Oral delivery remains a challenge for poorly permeable hydrophilic macromolecules. Poly(amido amine) (PAMAM) dendrimers have shown potential for their possible oral delivery. Transepithelial transport of carboxyl-terminated G3.5 and amine-terminated G4 PAMAM dendrimers was assessed using isolated rat jejunal mucosae m...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5004465
更新日期：2014-08-11 00:00:00
abstract：:Deproteinized natural rubber latex (DPNR-latex) was treated with lipase and phosphatase in order to analyze the structure of the chain-end group (alpha-terminal). The enzymatic treatment decreased the content of long-chain fatty acid ester groups in DPNR from about 6 to 2 mol per rubber molecule. The molecular weight ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm058003x
更新日期：2005-07-01 00:00:00
abstract：:Softwood and hardwood celluloses were oxidized by 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation. The TEMPO-oxidized cellulose fibers were converted to transparent dispersions in water, which consisted of individual nanofibers 3-4 nm in width. Films were then prepared from the TEMPO-oxidized c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801065u
更新日期：2009-01-12 00:00:00
abstract：:The maintenance of hepatocyte phenotype and functions remains as a great challenge in the generation of functional liver tissue and in vitro model for drug metabolism studies. The use of hepatocyte coculture systems plays essential roles in the establishment of cell-cell and cell-extracellular matrix communications si...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401926k
更新日期：2014-03-10 00:00:00
abstract：:We have constructed a tandem gene expression cassette containing three Ralstonia eutropha poly[(R)-3-hydroxybutyrate] (PHB) synthesis genes under the control of the Pichia pastoris glyceraldehyde-3-phosphate promoter and the green fluorescent protein (Gfp) under the control of the P. pastoris alcohol oxidase promoter....
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0494141
更新日期：2005-03-01 00:00:00
abstract：:Dextran surface grafting density was systematically varied via a two-step process involving SiO(2) amination by aminopropyltriethoxy silane (APTES) followed by oxidized dextran (M(w) = 110 kDa) chemisorption. Dextran oxidation kinetics with sodium metaperiodate (NaIO(4)) were quantified by (1)H NMR and pH measurements...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050601o
更新日期：2006-02-01 00:00:00
abstract：:The manufacture of biodegradable materials from agricultural sources is a real challenge, because of environmental concerns and the need to make full use of resources. Cottonseed is an important protein source that could be used in nonfood applications, as a substitute for synthetic polymers. For the first time, the v...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015525d
更新日期：2001-01-01 00:00:00
abstract：:Infectious diseases remain one of the leading causes of death worldwide despite the tremendous effort devoted to the design and development of antimicrobial agents. However, the decrease in the effectiveness of some antibiotics is often associated with the development of drug resistance by pathogen. This leads to an u...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00716
更新日期：2015-09-14 00:00:00
abstract：:The development of improved cryopreservative materials is necessary to enable complete recovery of living cells and tissue after frozen storage. Remarkably, poly(vinyl alcohol) (PVA) displays some of the same cryoprotective properties as many antifreeze proteins found in cold tolerant organisms. In particular, PVA is ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01502
更新日期：2018-01-08 00:00:00
abstract：:A layer-by-layer film composed of DNA and inorganic zirconium ion (Zr(4+)) was fabricated on the surface of gold thin film, and an electric field triggered disintegration of the multilayer film was studied by using electrochemical surface plasmon resonance (EC-SPR). EC-SPR results demonstrated that the film was disass...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800766t
更新日期：2008-10-01 00:00:00
abstract：:Intraneuronal aggregation of the intrinsically disordered protein α-synuclein is at the core of Parkinson's disease and related neurodegenerative disorders. Several reports show that the concentration of salts in the medium heavily affects its aggregation rate and fibril morphology, but a characterization of the indiv...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01292
更新日期：2020-12-14 00:00:00
abstract：:Encapsulation of hydrophilic cancer drugs in polymeric nanocapsules was achieved in a one-pot process via the inverse miniemulsion periphery RAFT polymerization (IMEPP) approach. The chosen guest molecule was gemcitabine hydrochloride, which is used as the first-line treatment of pancreatic cancer. The resulting nanoc...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00545
更新日期：2015-07-13 00:00:00
abstract：:A scanning laser system has been used to generate three-dimensional trimethylolpropane trimethacrylate (TRIM) cross-linked poly(2-hydroxylethyl methacrylate) polymer microstructures through azo-bis(isobutyro)nitrile (AIBN) photopolymerization using a 20 x 0.5 NA microscope objective and 365 nm laser excitation. Macrop...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0506952
更新日期：2006-03-01 00:00:00
abstract：:A novel aptamer modified thermosensitive liposome was designed as an efficient magnetic resonance imaging probe. In this paper, Gd-DTPA was encapsulated into an optimized thermosensitive liposome (TSL) formulation, followed by conjugation with AS1411 for specific targeting against tumor cells that overexpress nucleoli...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00250
更新日期：2015-09-14 00:00:00
abstract：:Algae constitute a largely available, low value material from renewable resources of marine origin to be used for the production of eco-compatible composites. Fibers of the green alga Ulva armoricana from the French coast were positively evaluated for the production of composites with a hydrophilic, eco-compatible pol...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701041e
更新日期：2008-03-01 00:00:00
abstract：:External magnetic field is a powerful approach to induce orientational order in originally disordered suspensions of magneto-responsive anisotropic particles. By small angle neutron scattering and optical birefringence measurement technology, we investigated the effect of magnetic field on the spatial ordering of hybr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00539
更新日期：2016-08-08 00:00:00
abstract：:Multifunctional cellulose nanocrystals have been synthesized and applied as a new type of glyconanomaterial in lectin binding and bacterial imaging. The cellulose nanocrystals were prepared by TEMPO-mediated oxidation and acidic hydrolysis, followed by functionalization with a quinolone fluorophore and carbohydrate li...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00227
更新日期：2015-04-13 00:00:00
abstract：:Ambidextrous supergelators are developed through structure-gelation screening of rationally designed cyclic dipeptides (CDPs). The organo- and hydrogels of CDPs were thoroughly characterized by their minimal gelation concentration (MGC) for organic and aqueous solvents, thermal stability (Tg), and viscoelastic propert...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00924
更新日期：2017-11-13 00:00:00
abstract：:A new synthetic route to beta-cyclodextrin-linked hyaluronic acid (HA-CD) was developed. This was based on the preparation of a HA derivative selectively modified with adipic dihydrazide (HA-ADH) and a beta-cyclodextrin derivative possessing an aldehyde function on the primary face, followed by their coupling by a red...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0507094
更新日期：2006-03-01 00:00:00
