Abstract：
:Nanoparticles with high aspect ratios have favorable attributes for drug delivery and bioimaging applications based on their enhanced tissue penetration and tumor homing properties. Here, we investigated a novel filamentous viral nanoparticle (VNP) based on the Pepino mosaic virus (PepMV), a relative of the established platform Potato virus X (PVX). We studied the chemical reactivity of PepMV, produced fluorescent versions of PepMV and PVX, and then evaluated their biodistribution in mouse tumor models. We found that PepMV can be conjugated to various small chemical modifiers including fluorescent probes via the amine groups of surface-exposed lysine residues, yielding VNPs carrying payloads of up to 1600 modifiers per particle. Although PepMV and PVX share similarities in particle size and shape, PepMV achieved enhanced tumor homing and less nonspecific tissue distribution compared to PVX in mouse models of triple negative breast cancer and ovarian cancer. In conclusion, PepMV provides a novel tool for nanomedical research but more research is needed to fully exploit the potential of plant VNPs for health applications.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Le DHT,Méndez-López E,Wang C,Commandeur U,Aranda MA,Steinmetz NFdoi
10.1021/acs.biomac.8b01365subject
Has Abstractpub_date
2019-01-14 00:00:00pages
469-477issue
1eissn
1525-7797issn
1526-4602journal_volume
20pub_type
杂志文章abstract：:Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2009075
更新日期：2011-09-12 00:00:00
abstract：:A drying environment is always a proposition faced by dynamic living organisms using water, which are driven by biopolymer-based micro- and macrostructures. Here, we introduce a drying process for aqueous liquid crystalline (LC) solutions composed of biopolymer with extremely high molecular weight components such as p...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00302
更新日期：2016-06-13 00:00:00
abstract：:The introduction of polymers into a chiral nematic cellulose nanocrystal (CNC) matrix allows for the tuning of optical and mechanical properties, enabling the development of responsive photonic materials. In this study, we explored the incorporation of hydroxypropyl cellulose (HPC) into a CNC film prepared by slow eva...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00056
更新日期：2020-03-09 00:00:00
abstract：:It is recognized that topographical features such as ridges and grooves can dramatically influence cell phenotype, motivating the development of substrates with precisely biomimetic topography for study of the influence on cultured cells. Intestinal basement membrane topography has been precisely replicated using plas...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100209b
更新日期：2010-06-14 00:00:00
abstract：:Sjögren's syndrome (SS) is an autoimmune disease associated with severe exocrinopathy, which is characterized by profound lymphocytic infiltration (dacryoadenitis) and loss of function of the tear-producing lacrimal glands (LGs). Systemic administration of Rapamycin (Rapa) significantly reduces LG inflammation in the ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01468
更新日期：2020-12-27 00:00:00
abstract：:Structural properties of different polypeptide multilayer nanofilms fabricated at neutral pH have been analyzed by UV spectroscopy, circular dichroism spectroscopy (CD), and Fourier-transform infrared spectroscopy (FTIR). The various peptides studied exhibit a strong tendency to adopt a beta sheet conformation in the ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700967k
更新日期：2008-01-01 00:00:00
abstract：:Adhesive hydrogels were prepared by copolymerizing dopamine methacrylamide with either acrylic acid (AAc) or N-(3-aminopropyl)methacrylamide hydrochloride (APMH). The effect of incorporating the anionic and cationic side chains on the oxidation state of catechol was characterized using the FOX assay to track the produ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b01311
更新日期：2018-05-14 00:00:00
abstract：:In a previous study, we have reported chemical synthesis of novel aliphatic poly(butylene succinate-co-cyclic carbonate) P(BS-co-CC)s bearing various functionalizable carbonate building blocks, and this work will continue to present our new studies on their enzymatic degradation and in vitro cell biocompatibility assa...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049705+
更新日期：2004-11-01 00:00:00
abstract：:Self-cross-linking polyelectrolytes are used to strengthen the surface of calcium alginate beads for cell encapsulation. Poly([2-(methacryloyloxy)ethyl]trimethylammonium chloride), containing 30 mol % 2-aminoethyl methacrylate, and poly(sodium methacrylate), containing 30 mol % 2-(methacryloyloxy)ethyl acetoacetate, w...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800580c
更新日期：2008-09-01 00:00:00
abstract：:The oxidation-responsive behaviors of OEGylated poly-L-cysteine homopolypeptides, that is, poly(L-EG(x)MA-C)n, were investigated. These poly-L-cysteine derivatives adopted mixed conformation in water, in which the β-sheet accounted for a significant proportion. Upon oxidation, the thioethers in polypeptide side chains...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5000554
更新日期：2014-03-10 00:00:00
abstract：:The molecular recognition features of a DNA-sensitive fluorescent bioconjugate capable of targeting a specific DNA sequence with high efficiency are described. The bioconjugate combines a polypeptide from the Tc3 transposase DNA-binding domain with the dsDNA-sensitive fluorophore thiazole orange. Fluorescence spectros...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070334h
更新日期：2007-11-01 00:00:00
abstract：:We examine the protein distribution within an electrospun polymer nanofiber using polyvinyl alcohol and bovine serum albumin as a model system. We hypothesize that the location of the protein within the nanofiber can be controlled by carefully selecting the pH and the applied polarity of the electric field as the pH a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2017146
更新日期：2012-05-14 00:00:00
abstract：:Polymer therapeutics, including polymeric drugs and polymer-protein conjugates, are clinically established as first-generation nanomedicines. Knowing that the coiled-coil peptide motif is fundamentally important in the regulation of many cellular and pathological processes, the aim of these studies was to examine the ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100843e
更新日期：2011-01-10 00:00:00
abstract：:A series of copolymers of trimethylene carbonate (TMC) and L-lactide (LLA) were synthesized and evaluated as scaffolds for the production of artificial blood vessels. The polymers were end-functionalized with acrylate, cast into films, and cross-linked using UV light. The mechanical, degradation, and biocompatibility ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201291e
更新日期：2011-11-14 00:00:00
abstract：:Nucleic acids have been engineered to participate in a wide variety of tasks. Among them, the enzyme-free amplification modes, enzyme-free DNA circuits (EFDCs), and hybridization chain reactions (HCRs) have been widely applied in a series of studies of bioanalysis. We demonstrated here an ultrasensitive hairpin probe-...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00959
更新日期：2015-11-09 00:00:00
abstract：:The need for polymers for high-end applications, coupled with the desire to mimic nature's macromolecular machinery fuels the development of innovative synthetic strategies every year. The recently acquired macromolecular-synthetic tools increase the precision and enable the synthesis of polymers with high control and...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/acs.biomac.5b01698
更新日期：2016-03-14 00:00:00
abstract：:Despite numerous strategies involving dynamic covalent bonds to produce self-healing hydrogels with similar frequency-dependent stiffness to native tissues, it remains challenging to use biologically relevant thiol/disulfide exchange to confer such properties to polymeric networks. Herein, we report a new method based...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00980
更新日期：2015-11-09 00:00:00
abstract：:Hydrogels derived from resilin-like polypeptides (RLPs) have shown outstanding mechanical resilience and cytocompatibility; expanding the versatility of RLP-based materials via conjugation with other polypeptides and polymers would offer great promise in the design of a range of materials. Here, we present an investig...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01255
更新日期：2016-01-11 00:00:00
abstract：:This paper shows a simple and effective way to avoid the dissociation of multimeric enzymes by coating their surface with a large cationic polymer (e.g., polyethylenimine (PEI)) by ionic exchange. As model enzymes, glutamate dehydrogenase (GDH) from Thermus thermophilus and formate dehydrogenase (FDH) from Pseudomonas...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801162e
更新日期：2009-04-13 00:00:00
abstract：:A series of chitosan/chitin samples with DA's varying between 5.2 and 89% was prepared from the reacetylation under soft conditions of a unique chitosan sample allowing the preservation of the chain distribution. The study of the variation of pH for the same concentration of amine groups, at different ionic strengths,...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm015531+
更新日期：2001-10-01 00:00:00
abstract：:Synthetic and natural mucoadhesive biomaterials in optimized galenical formulations are potentially useful for the transmucosal delivery of active ingredients to improve their localized and prolonged effects. Chitosans (CS) have potent mucoadhesive characteristics, but the exact mechanisms underpinning such interactio...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm5007954
更新日期：2014-10-13 00:00:00
abstract：:A new, convenient pathway is developed to synthesize highly hydrolytically labile poly(ortho ester amide) (POEA) copolymers that overcomes some of the major weaknesses of the traditional methods of synthesizing poly(ortho esters) and their derivatives. A diamine monomer containing a built-in, stabilized ortho ester gr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9000475
更新日期：2009-04-13 00:00:00
abstract：:Poly(epsilon-caprolactone)/polylactide blend (PCL/PLA) is an interesting biomaterial because PCL and PLA present good complementarity in their physical properties and biodegradability. However, the thermodynamic incompatibility between two component polymers restricts further applications of their blend. In this work,...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801183f
更新日期：2009-02-09 00:00:00
abstract：:Reversible addition-fragmentation chain transfer (RAFT) radical polymerization, mediated by 4-cyanopentanoic acid dithiobenzoate and 4,4'-azobis(4-cyanovaleric acid) (V-501) in water at 70 degrees C, of biocompatible 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) yields a macro-chain transfer agent (CTA) that was em...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8014945
更新日期：2009-04-13 00:00:00
abstract：:Laminin is a heterotrimeric glycoprotein with a key role in the formation and maintenance of the basement membrane architecture and properties, as well as on the modulation of several biological functions, including cell adhesion, migration, differentiation and matrix-mediated signaling. In the central nervous system ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01319
更新日期：2020-02-10 00:00:00
abstract：:A revised crystal structure for mercerized cellulose based on high-resolution synchrotron X-ray data collected from ramie fibers is reported (space group P2(1), a = 8.10(3) A, b = 9.03(3) A, c = 10.31(5) A, gamma = 117.10(5) degrees; 751 reflections in 304 composite spots; theta < 21.11 degrees; lambda = 0.7208 A; LAL...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm005612q
更新日期：2001-07-01 00:00:00
abstract：:Supramolecular nanogel, a physically cross-linked nanosize hydrogel, spontaneously self-assembles in aqueous solution via secondary interactions and is thus of great interest in nanomedicine as a drug carrier. We developed a versatile method for supramolecular nanogel self-assembled by electrostatic interaction betwee...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400846h
更新日期：2013-10-14 00:00:00
abstract：:Homocystinuria due to loss of cystathionine beta-synthase (CBS) causes accumulation of homocysteine and depletion of cysteine. Current treatments are suboptimal, and thus the development of an enzyme replacement therapy based on PEGylated human truncated CBS (PEG-CBS) has been initiated. Attenuation of potency was obs...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00154
更新日期：2017-06-12 00:00:00
abstract：:Feasibility of using amphiphilic block copolymers composed of polylactide (PLA) and poly(ethylene oxide) (PEO) blocks for biomimetic surface modification of polylactide-based biomaterials for tissue engineering was investigated. PEO-b-PLA copolymers were deposited on the PLA surface from a solution in PEO-selective so...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900889b
更新日期：2010-01-11 00:00:00
abstract：:Textile chitosan fiber scaffolds were developed and tested in terms of biocompatibility for human bone marrow stromal cells (hBMSCs). A part of the scaffolds was further modified by coating with fibrillar collagen type I in order to biologize the surface. hBMSCs of two donors were used for cell culture experiments in ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900172m
更新日期：2009-05-11 00:00:00
