Abstract:
:The formation of a suitable extracellular matrix (ECM) that promotes cell adhesion, organization, and proliferation is essential within biomaterial scaffolds for tissue engineering applications. In this work, short elastin mimetic peptide sequences, EM-19 and EM-23, were engineered to mimic the active motifs of human elastin in hopes that they can stimulate ECM development in synthetic polymer scaffolds. Each peptide was incubated with human aortic smooth muscle cells (SMCs) and elastin and desmosine production were quantified after 48 h. EM-19 inhibited elastin production through competitive binding phenomena with the elastin binding protein (EBP), whereas EM-23, which contains an RGDS domain, induces recovery of elastin production at higher concentrations, alluding to a higher binding affinity for the integrins than for the EBP and the involvement of integrins in elastin production. Colocalization of each peptide with the elastin matrix was confirmed using immunofluorescent techniques. Our data suggest that with appropriate cell-binding motifs, we can simulate the cross-linking of tropoelastin into the developing elastin matrix using short peptide sequences. The potential for increased cell adhesion and the incorporation of elastin chains into tissue engineering scaffolds make these peptides attractive bioactive moieties that can easily be incorporated into synthetic biomaterials to induce ECM formation.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Patel D,Menon R,Taite LJdoi
10.1021/bm101214fsubject
Has Abstractpub_date
2011-02-14 00:00:00pages
432-40issue
2eissn
1525-7797issn
1526-4602journal_volume
12pub_type
杂志文章abstract::(1,3)(1,4)-β-D-Glucan (mixed-linkage glucan or MLG), a characteristic hemicellulose in primary cell walls of grasses, was investigated to determine both its role in cell walls and its interaction with cellulose and other cell wall polysaccharides in vitro. Binding isotherms showed that MLG adsorption onto microcrystal...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm5001247
更新日期:2014-05-12 00:00:00
abstract::Atomic force microscopy (AFM) was used to assess a new chimeric protein consisting of a fusion protein of the consensus repeat for Nephila clavipes spider dragline protein and bone sialoprotein (6mer+BSP). The elastic modulus of this protein in film form was assessed through force curves, and film surface roughness wa...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2000605
更新日期:2011-05-09 00:00:00
abstract::External magnetic field is a powerful approach to induce orientational order in originally disordered suspensions of magneto-responsive anisotropic particles. By small angle neutron scattering and optical birefringence measurement technology, we investigated the effect of magnetic field on the spatial ordering of hybr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b00539
更新日期:2016-08-08 00:00:00
abstract::The single-coat protein (CP) of bacteriophage Qβ self-assembles into T = 3 icosahedral virus-like particles (VLPs), of interest for a wide range of applications. These VLPs are very stable, but identification of the specific molecular determinants of this stability is lacking. To investigate these determinants along w...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300590x
更新日期:2012-08-13 00:00:00
abstract::Nitrilotriacetic amine (NTA)-functionalized nanoparticles obtained by aqueous polymerization-induced self-assembly (PISA) are introduced as immobilization supports for polyhistidine-functionalized (His-tagged) enzymes. A novel initiator for nitroxide-mediated polymerization based on the nitroxide SG1 and carrying a pr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00677
更新日期:2017-09-11 00:00:00
abstract::Deproteinized natural rubber latex (DPNR-latex) was treated with lipase and phosphatase in order to analyze the structure of the chain-end group (alpha-terminal). The enzymatic treatment decreased the content of long-chain fatty acid ester groups in DPNR from about 6 to 2 mol per rubber molecule. The molecular weight ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm058003x
更新日期:2005-07-01 00:00:00
abstract::Peptide ligation is an indispensable step in the chemical synthesis of target peptides and proteins that are difficult to synthesize at once by a solid-phase synthesis. The ligation reaction is generally conducted with two peptide fragments at a high aqueous concentration to increase the reaction rate; however, this o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01655
更新日期:2019-03-11 00:00:00
abstract::Hydrogels with pH-sensitive poly(acrylic acid) (PAAc) chains and biodegradable acryloyl-poly(-caprolactone)-2-hydroxylethyl methacrylate (AC-PCL-HEMA) chains were designed and synthesized. The morphology of hydrogel was observed by scanning electron microscopy. The degradation of the hydrogel in the presence of Pseudo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7010328
更新日期:2008-04-01 00:00:00
abstract::A revised crystal structure for mercerized cellulose based on high-resolution synchrotron X-ray data collected from ramie fibers is reported (space group P2(1), a = 8.10(3) A, b = 9.03(3) A, c = 10.31(5) A, gamma = 117.10(5) degrees; 751 reflections in 304 composite spots; theta < 21.11 degrees; lambda = 0.7208 A; LAL...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm005612q
更新日期:2001-07-01 00:00:00
abstract::Poly(lactic acid) (PLA) copolymers having a significantly higher glass transition temperature (T(g)) than that of high molar mass PLA homopolymers (typically 60 +/- 5 degrees C) were prepared. Lactic acid was copolymerized with 1,4:3,6-dianhydro-D-glucitol (isosorbide, ISB) and succinic acid (SA-2), 1,2,3,4-butanetetr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901338n
更新日期:2010-05-10 00:00:00
abstract::This study highlights the advantages of chondroitin sulfate (CS) as a sublayer combining selective low-fouling properties, low-platelet adhesion and pro-adhesive properties on endothelial cells, making CS promising for vascular graft applications. These properties were evaluated by comparing CS with well-known low-fou...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm5003762
更新日期:2014-07-14 00:00:00
abstract::Branched amphiphilic copolymers were synthesized through the reversible addition-fragmentation chain transfer (RAFT) chain extension of a poly(methyl acrylate) macro-chain transfer agent using a protected galactose monomer and a polymerizable chain transfer agent branching unit. After galactose deprotection, the copol...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501482q
更新日期:2015-01-12 00:00:00
abstract::The outcome for glioblastoma patients remains dismal for its invariably recrudesces within 2 cm of the resection cavity. Local immunotherapy has the potential to eradicate the residual infiltrative component of these tumors. Here, we report the development of a biodegradable hydrogel containing therapeutic T lymphocyt...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500502n
更新日期:2014-07-14 00:00:00
abstract::Development of versatile nanoscale platforms for cancer diagnosis and therapy is of great importance for applications in translational medicine. In this work, we present the use of γ-polyglutamic acid (γ-PGA) nanogels (NGs) to load polypyrrole (PPy) for thermal/photoacoustic (PA) imaging and radiotherapy (RT)-sensitiz...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00184
更新日期:2018-06-11 00:00:00
abstract::Well-defined linear poly(acryloyl glucosamine) (PAGA) exhibiting molar masses ranging from 3 to 120 K and low polydispersities have been prepared via reversible addition-fragmentation chain transfer polymerization (RAFT) in aqueous solution without recourse to protecting group chemistry. The livingness of the process ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0506086
更新日期:2006-01-01 00:00:00
abstract::In this paper we extend our previous experimental work on interaction between polyelectrolytes and liposomes. First, the adsorption of chitosan and alkylated chitosan (cationic polyelectrolytes) with different alkyl chain lengths on lipid membranes of liposomes is examined. The amount of both chitosans adsorbed remain...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800400y
更新日期:2008-08-01 00:00:00
abstract::A way to convert the volume change of a biochemo-mechanical gel into the change in liquid column length was developed. Our trial sensor device consisted of a small compartment for incorporating the gel, a flow channel with a filled dye solution, and a poly(dimethylsiloxane) (PDMS) diaphragm by which the gel and the dy...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034331q
更新日期:2004-03-01 00:00:00
abstract::Stimuli-responsive hydrogels have great potentials in biomedical and biotechnological applications. Due to the advantages of precise control over molecular weight and being biodegradable, protein-based hydrogels and their applications have been extensively studied. However, protein hydrogels with dual thermosensitive ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01231
更新日期:2015-11-09 00:00:00
abstract::For developing broader application of biobased polymers, graft copolymers and comb polymers having poly(lactic acid) (PLA) side chains have been synthesized by using a macromonomer technique. PLA macromonomers (MMm) having a methacryloyl polymerizable group with different PLA chain length with an average length m = 4,...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301212a
更新日期:2012-11-12 00:00:00
abstract::In the fields of surgery and regenerative medicine, it is crucial to understand the interactions of proteins with the biomaterials used as implants. Protein adsorption directly influences cell-material interactions in vivo and, as a result, regulates, for example, cell adhesion on the surface of the implant. Therefore...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500750v
更新日期:2014-07-14 00:00:00
abstract::Dragline silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301110s
更新日期:2012-12-10 00:00:00
abstract::A novel family of amphiphilic temperature- and pH-sensitive poly(organophosphazenes) with varying ratios of ethylene oxide, alkyl chains and free acid units was synthesized by living cationic polymerization. Depending on their composition, these poly(organophosphazenes) exhibited lower critical solution temperatures r...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0400527
更新日期:2004-11-01 00:00:00
abstract::Cellular uptake and intracellular targeting to specific organelles are key events in the cellular processing of nanomaterials. Herein, we perform a detailed structure-property relationship study on carboxylic acid-side-chain-bearing polyacrylates to provide design criteria for the manipulation of their cellular intera...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00244
更新日期:2020-08-10 00:00:00
abstract::The leading cause of death in the United States is cardiovascular disease. The majority of these cases result from heart failure post-myocardial infarction (MI). We present data providing evidence for use of acetalated dextran (AcDex) microparticles as a delivery vehicle for therapeutics to the heart post-MI. We harne...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401050j
更新日期:2013-11-11 00:00:00
abstract::The polarity of polymer-actin complexes obtained by mixing F-actin with synthetic polymers carrying positive charges such as poly(L-lysine), x,y-ionene bromide polymers, and poly(N-[3-(dimethylamino)propyl]acrylamide) (PDMAPAA-Q) have been investigated. Actin complexes formed with poly(L-lysine) and PDMAPAA-Q, which c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0494038
更新日期:2005-03-01 00:00:00
abstract::Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2009075
更新日期:2011-09-12 00:00:00
abstract::Magnetosomes are natural magnetic nanoparticles with exceptional properties that are synthesized in magnetotactic bacteria by a highly regulated biomineralization process. Their usability in many applications could be further improved by encapsulation in biocompatible polymers. In this study, we explored the productio...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01749
更新日期:2018-03-12 00:00:00
abstract::In this work we describe the application of amphiphilic N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymers as polymeric surfactants in miniemulsion techniques. HPMA-based copolymers with different ratios of HPMA (hydrophilic) to laurylmethacrylate (LMA; hydrophobic) units were synthesized by RAFT polymerization...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301453g
更新日期:2012-12-10 00:00:00
abstract::Fibrin sealants are a type of soft tissue adhesive that employs biochemical reactions from the late stages of the blood coagulation cascade. Intrinsic to these adhesives are a structural protein and a transglutaminase crosslinking enzyme. We are investigating an alternative biomimetic adhesive based on gelatin and a c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034529a
更新日期:2004-07-01 00:00:00
abstract::Computationally designed peptides form desired antiparallel, tetrameric coiled-coil bundles that hierarchically assemble into a variety of well-controlled nanostructures depending on aqueous solution conditions. The bundles selectively self-assemble into different structures: nanotubes, platelets, or needle-like struc...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01163
更新日期:2018-11-12 00:00:00