Abstract:
:Magnetosomes are natural magnetic nanoparticles with exceptional properties that are synthesized in magnetotactic bacteria by a highly regulated biomineralization process. Their usability in many applications could be further improved by encapsulation in biocompatible polymers. In this study, we explored the production of spider silk-inspired peptides on magnetosomes of the alphaproteobacterium Magnetospirillum gryphiswaldense. Genetic fusion of different silk sequence-like variants to abundant magnetosome membrane proteins enhanced magnetite biomineralization and caused the formation of a proteinaceous capsule, which increased the colloidal stability of isolated particles. Furthermore, we show that spider silk peptides fused to a magnetosome membrane protein can be used as seeds for silk fibril growth on the magnetosome surface. In summary, we demonstrate that the combination of two different biogenic materials generates a genetically encoded hybrid composite with engineerable new properties and enhanced potential for various applications.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Mickoleit F,Borkner CB,Toro-Nahuelpan M,Herold HM,Maier DS,Plitzko JM,Scheibel T,Schüler Ddoi
10.1021/acs.biomac.7b01749subject
Has Abstractpub_date
2018-03-12 00:00:00pages
962-972issue
3eissn
1525-7797issn
1526-4602journal_volume
19pub_type
杂志文章abstract::This paper introduces a chitosan microneedle patch for efficient and sustained transdermal delivery of hydrophilic macromolecules. Chitosan microneedles have sufficient mechanical strength to be inserted in vitro into porcine skin at approximately 250 μm in depth and in vivo into rat skin at approximately 200 μm in de...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301293d
更新日期:2012-12-10 00:00:00
abstract::In this work, biodegradable branched polycationic polymers were synthesized by Michael addition polymerization from different amine monomers and the triacrylate monomer trimethylolpropane triacrylate. The polymers varied in the number of amines that dissociate in different pH ranges, which are considered to be benefic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901147k
更新日期:2010-03-08 00:00:00
abstract::On-line size-exclusion chromatography monitoring of potato phosphorylase catalyzed amylose synthesis--starting from alpha-D-glucose-1-P and maltohexaose--revealed rather monodisperse amylose populations. In the presence of lipids, amylose-lipid complexes spontaneously formed and precipitated. They were recovered by ce...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0502011
更新日期:2005-09-01 00:00:00
abstract::Water-soluble, degradable polymers based on poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) with low cytotoxicity and good p-DNA transfection efficiency are highlighted in this article. To solve the nondegradability issue of PDMAEMA, new polymers based on DMAEMA and 5,6-benzo-2-methylene-1,3-dioxepane (BMDO) for g...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2015174
更新日期:2012-02-13 00:00:00
abstract::The supramolecular organization of self-assembled stoichiometric complexes formed by dodecyltrimethylammonium cations and ionized poly(gamma-glutamic) acid has been investigated in chloroform solution. Atomistic molecular dynamics simulations have been performed considering three different starting conformations for t...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060927c
更新日期:2007-02-01 00:00:00
abstract::Hydrophobic methacrylic monomers were polymerized in aqueous media using methylated (1.8)-beta-cyclodextrin (MeCD) additives. Hydrophobic monomers tert-butyl methacrylate (tBuMA), cyclohexyl methacrylate (CMA), and 2-ethylhexyl methacrylate (2EHMA) were each dissolved in chloroform with MeCD. Chloroform was then evapo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0055706
更新日期:2000-01-01 00:00:00
abstract::Combining molecular weight distribution (MWD) data for linear chains of debranched starch from capillary electrophoresis and from size exclusion chromatography (SEC) with detection by differential refractive index and by multi-angle laser light scattering has enabled Mark-Houwink parameters to be determined for linear...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050041t
更新日期:2005-07-01 00:00:00
abstract::Water soluble polymer anticancer conjugates can improve the pharmacokinetics of covalently bound drugs by limiting cellular uptake to the endocytic route, thus prolonging plasma circulation time and consequently facilitating tumor targeting by the enhanced permeability and retention (EPR) effect. Many of the first gen...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049381p
更新日期:2005-03-01 00:00:00
abstract::This paper reports the rheological behavior of chitosan solutions that have been cross-linked with different amounts of genipin, at body temperature and physiological pH. The effect of the cross-linker loading on the rheological properties of hydrogels has been evaluated. The oscillatory time sweep method was used to ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700762w
更新日期:2007-12-01 00:00:00
abstract::Polyamines are essential molecules supporting the structure, conformation, and function of nucleic acids and proteins. We studied stereoisomers of alpha,alpha'-dimethylated spermine [(R,R)-Me(2)Spm, (S,S)-Me(2)Spm, (R,S)-Me(2)Spm] for their ability to provoke DNA condensation and protect DNA from damage. (R,R)- and (R...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900958c
更新日期:2010-01-11 00:00:00
abstract::Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049941k
更新日期:2004-07-01 00:00:00
abstract::There is a lack of hydrogel materials whose properties can be tuned at the point of use. Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds because of their low mechanical strength. Their mechanics can be improved through cross-linking and chem...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01251
更新日期:2016-03-14 00:00:00
abstract::Oligopeptide hydrogels are emerging as useful matrices for cell culture with commercial products on the market, but L-oligopeptides are labile to proteases. An obvious solution is to create D-oligopeptide hydrogels, which lack enzymatic recognition. However, D-oligopeptide matrices do not support cell growth as well a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301598g
更新日期:2013-02-11 00:00:00
abstract::The morphologies of several ionotropic alginate hydrogels and aerogels were investigated by SAXS according to the nature of the divalent metal cation (Mn(2+), Co(2+), Zn(2+), Cu(2+)) and the guluronic fraction of the alginate. All alginate hydrogel and aerogel samples show isotropic small-angle X-ray scattering. Gelat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201477g
更新日期:2012-01-09 00:00:00
abstract::Textile chitosan fiber scaffolds were developed and tested in terms of biocompatibility for human bone marrow stromal cells (hBMSCs). A part of the scaffolds was further modified by coating with fibrillar collagen type I in order to biologize the surface. hBMSCs of two donors were used for cell culture experiments in ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900172m
更新日期:2009-05-11 00:00:00
abstract::The order of assembly of a magnetic nanoparticle (MNP) vector comprised of the same components (MNP, PEI, and plasmid DNA) on entry mechanism, intracellular localization, and viability of BHK21 cells was investigated. Cellular uptake measurements under four different uptake inhibiting conditions, such as low temperatu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100748p
更新日期:2010-09-13 00:00:00
abstract::The elegant elasticity and toughness of muscles that are controlled by myofilament sliding, highly elastic springlike properties of titin, and Ca2+-induced conformational change of the troponin complex have been a source of inspiration to develop advanced materials for simulating elastic muscle motion. Herein, a highl...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01002
更新日期:2020-10-12 00:00:00
abstract::A novel family of amphiphilic temperature- and pH-sensitive poly(organophosphazenes) with varying ratios of ethylene oxide, alkyl chains and free acid units was synthesized by living cationic polymerization. Depending on their composition, these poly(organophosphazenes) exhibited lower critical solution temperatures r...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0400527
更新日期:2004-11-01 00:00:00
abstract::The bioconjugation of proteins and peptides with synthetic polymers is a promising method for tailoring the chemical, biological, and physical properties of both the polymeric and protein-based components. Here, we describe macromolecular assemblies of polyethylene glycol-coiled-coil alternating multiblock polymers gu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900474k
更新日期:2009-10-12 00:00:00
abstract::The detection of phase separation and identification of miscibility in biopolymer blends is an important aspect for the improvement of their physical properties. In this article, the phase separation in blends of poly(3-hydroxybutyrate) (PHB) with poly(L-lactic acid) (PLA) and poly(epsilon-caprolactone) (PCL), respect...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701035p
更新日期:2008-02-01 00:00:00
abstract::A His-tagged coiled coil stem loop peptide with stable secondary structure was designed and biosynthesized. A series of oligopeptides related to the EBV envelope glycoprotein 350/220 N-terminal nonapeptide as potential CD21 receptor-binding epitopes were engineered into the loop region of the peptide scaffold. It was ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm015606+
更新日期:2002-05-01 00:00:00
abstract::Ruthenium(II) tris(bipyridine)-centered poly(ethylenimine) (Ru PEI) was synthesized via acid hydrolysis of Ru tris(bipyridine)-centered poly(2-ethyl-2-oxazoline) (Ru PEOX), and the luminescence, DNA entrapment, and transfection efficiencies were evaluated. Emission maxima for Ru PEI samples are red-shifted compared to...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700481h
更新日期:2007-09-01 00:00:00
abstract::Chitosan hydrogel microspheres were obtained by cross-linking chitosan in its inverse emulsion using genipin as cross-linker. The genipin-cross-linked chitosan microspheres (ChGp) swell significantly in water at pH values below 6.5 and shrink to a smaller extent at pH values above 6.5. ChGp microspheres bind heparin i...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800724q
更新日期:2008-11-01 00:00:00
abstract::A new series of cationic, thermo-sensitive, and biodegradable poly(L-lactide)-poly(2-ethyl-2-oxazoline)-poly(L-lactide) (PLLA-PEOz-PLLA) triblock copolymers were synthesized by ring-opening polymerization. With increasing molecular weight and crystallinity of hydrophobic PLLA blocks, the critical micellization concent...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034190s
更新日期:2003-11-01 00:00:00
abstract::Silk fibroin (SF) protein, produced by silkworm Bombyx mori, is a promising biomaterial, while sophorolipid (SL) is an amphiphilic functional biosurfactant synthesized by nonpathogenic yeast Candida bombicola. SL is a mixture of two forms, acidic (ASL) and lactonic (LSL), which when added to SF results in accelerated ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01069
更新日期:2016-10-10 00:00:00
abstract::Characterization of poly-3-hydroxybutyric acid (PHB) and poly-3-hydroxybutyric-co-valeric acid (PHBV, 13% valerate) in chloroform was performed using size exclusion chromatography coupled to a multi-angle light scattering detector (SEC-MALS). Absolute molar mass averages, molar mass distribution, and the radius of gyr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm030073l
更新日期:2004-03-01 00:00:00
abstract::Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was applied to investigate the interaction of bovine serum albumin (BSA) and fibrinogen with a biomedical-grade 316LVM stainless steel surface, in terms of the adsorption thermodynamics and adsorption-induced secondary structure changes of ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070289d
更新日期:2007-09-01 00:00:00
abstract::The polymerization of N-isopropylacrylamide (NIPAAm) with ionizable monomers results in pH-responsive lower critical solution temperature (LCST) polymer which works in an ionization-dependent manner. However, gradual ionization of the comonomer occurs at a broad pH range due to the electrostatic field generated by the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01465
更新日期:2019-04-08 00:00:00
abstract::A new α-amylase from Rhizomucor sp. (RA) was studied in detail due to its very efficient hydrolysis of raw starch granules at low temperature (32 °C). RA contains a starch binding domain (SBD) connected to the core amylase catalytic domain by a O-glycosylated linker. The mode of degradation of native maize starch gran...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100913z
更新日期:2011-01-10 00:00:00
abstract::In biomedicine, PEGylation is one of the most successful strategies to modify the physicochemical and biological properties of peptides, proteins, and other biomacromolecules. Because of the polydisperse nature of regular PEGs and limited PEGylation strategies, it is challenging to quantitatively fine-tune and accurat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01425
更新日期:2020-02-10 00:00:00