Presentation of epitopes on genetically engineered peptides and selection of lymphoma-targeting moieties based on epitope biorecognition.


:A His-tagged coiled coil stem loop peptide with stable secondary structure was designed and biosynthesized. A series of oligopeptides related to the EBV envelope glycoprotein 350/220 N-terminal nonapeptide as potential CD21 receptor-binding epitopes were engineered into the loop region of the peptide scaffold. It was shown that these peptides had a stable alpha-helical coiled coil structure and assumed a monomeric form in PBS. Biorecognition of the epitopes was studied by immobilizing the epitope-containing peptides on complexed Ni2+-containing surfaces through His-Ni2+ chelation and incubating with purified soluble CD21 receptor or CD21+ cells. The results showed that the potential epitopes bound to CD21 and CD21+ cells at different affinities depending on oligopeptide structures. This approach allows for the evaluation of epitope biorecognizabilities and the selection of optimal oligopeptides among sequences for use as targeting moieties in the design of new lymphoma-targeting polymeric drug carriers.






Tang A,Kopecek J





Has Abstract


2002-05-01 00:00:00














  • Nature of Amorphous Hydrophilic Block Affects Self-Assembly of an Artificial Viral Coat Polypeptide.

    abstract::Consensus motifs for sequences of both crystallizable and amorphous blocks in silks and natural structural analogues of silks vary widely. To design novel silklike polypeptides, an important question is therefore how the nature of either the crystallizable or the amorphous block affects the self-assembly and resulting...


    pub_type: 杂志文章


    authors: Willems L,van Westerveld L,Roberts S,Weitzhandler I,Calcines Cruz C,Hernandez-Garcia A,Chilkoti A,Mastrobattista E,van der Oost J,de Vries R

    更新日期:2019-10-14 00:00:00

  • Pectic polysaccharides from Panax ginseng as the antirotavirus principals in ginseng.

    abstract::To evaluate the antidiarrheal effect of ginseng, the active principals of ginseng were studied in vitro model of rotavirus infection, the leading cause of severe diarrhea. Two pectic polysaccharides, named as GP50-dHR (56.0 kDa) and GP50-eHR (77.0 kDa), were purified from hot water extract of ginseng by bioassay-linke...


    pub_type: 杂志文章


    authors: Baek SH,Lee JG,Park SY,Bae ON,Kim DH,Park JH

    更新日期:2010-08-09 00:00:00

  • Potato phosphorylase catalyzed synthesis of amylose-lipid complexes.

    abstract::On-line size-exclusion chromatography monitoring of potato phosphorylase catalyzed amylose synthesis--starting from alpha-D-glucose-1-P and maltohexaose--revealed rather monodisperse amylose populations. In the presence of lipids, amylose-lipid complexes spontaneously formed and precipitated. They were recovered by ce...


    pub_type: 杂志文章


    authors: Gelders GG,Goesaert H,Delcour JA

    更新日期:2005-09-01 00:00:00

  • Mechanism and kinetics of phase separation in a gelatin/maltodextrin mixture studied by small-angle light scattering.

    abstract::Phase separation mechanisms and kinetics were studied using small-angle light scattering in a gelatin/maltodextrin system where phase separation could be studied in both liquid and gelled states. Nucleation and growth or spinodal decomposition occurred, depending on the quench depth. The transition between the two mec...


    pub_type: 杂志文章


    authors: Butler MF

    更新日期:2002-07-01 00:00:00

  • Synthesis of a chitosan-dendrimer hybrid and its biodegradation.

    abstract::Chitosan-dendrimer hybrids having various functional groups such as carboxyl, ester, and poly(ethylene glycol) groups were prepared successfully using dendrimer acetal by reductive N-alkylation. The synthetic procedure could be accomplished by one-step reaction without organic solvent. The degree of substitution of de...


    pub_type: 杂志文章


    authors: Sashiwa H,Yajima H,Aiba S

    更新日期:2003-09-01 00:00:00

  • Synthesis of poly[(R)-3-hydroxybutyric acid) in the cytoplasm of Pichia pastoris under oxygen limitation.

    abstract::We have constructed a tandem gene expression cassette containing three Ralstonia eutropha poly[(R)-3-hydroxybutyrate] (PHB) synthesis genes under the control of the Pichia pastoris glyceraldehyde-3-phosphate promoter and the green fluorescent protein (Gfp) under the control of the P. pastoris alcohol oxidase promoter....


    pub_type: 杂志文章


    authors: Vijayasankaran N,Carlson R,Srienc F

    更新日期:2005-03-01 00:00:00

  • Investigation of layer-by-layer assembly of polyelectrolytes on fully functional human red blood cells in suspension for attenuated immune response.

    abstract::The encapsulation of live cells with polymeric coat-ings is a versatile approach to modulate or control the response cells to their environment. The layer-by-layer (LbL) self-assembly of nonimmunogenic polyelectrolytes is employed here to attenuate or suppress the binding of antibodies to live red blood cells (RBCs) a...


    pub_type: 杂志文章


    authors: Mansouri S,Merhi Y,Winnik FM,Tabrizian M

    更新日期:2011-03-14 00:00:00

  • Improved stability and antidiabetic potential of insulin containing folic acid functionalized polymer stabilized multilayered liposomes following oral administration.

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    pub_type: 杂志文章


    authors: Agrawal AK,Harde H,Thanki K,Jain S

    更新日期:2014-01-13 00:00:00

  • Enzymatic copolymerization alters the structure of unpolymerized mixtures of the biomimetic monomers: the amphiphilic decyl ester of L-tyrosine and L-tyrosineamide--an AFM investigation of nano- to micrometer-scale structure differences.

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    pub_type: 杂志文章


    authors: Marx KA,Lee JS,Sung C

    更新日期:2004-09-01 00:00:00

  • Wormlike Nanovector with Enhanced Drug Loading Using Blends of Biodegradable Block Copolymers.

    abstract::The application of nanoparticles comprising amphiphilic block copolymers for the delivery of drugs is a subject of great interest as they hold promise for more effective and selective therapies. In order to achieve this ambition, it is of critical importance to develop our understanding of the self-assembly mechanisms...


    pub_type: 杂志文章


    authors: Ridolfo R,Arends JJ,van Hest JCM,Williams DS

    更新日期:2020-06-08 00:00:00

  • Genetically Encoded Inverse Bolaamphiphiles.

    abstract::Developing new protein-based materials with a programmable assembly is of great scientific interest and technological importance. Inspired by nature's use of post-translational modifications (PTMs) to control the function and location of proteins, we have leveraged lipidation-the PTM of proteins with lipids-to synthes...


    pub_type: 杂志文章


    authors: Hossain MS,Liu X,Maynard TI,Mozhdehi D

    更新日期:2020-02-10 00:00:00

  • Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin.

    abstract::Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or kappa-carrageenan, activation with glutaraldehyde, glycidol, or epichlorohydrin, and addition of microorganisms followed by ce...


    pub_type: 杂志文章


    authors: Adriano WS,Mendonça DB,Rodrigues DS,Mammarella EJ,Giordano RL

    更新日期:2008-08-01 00:00:00

  • Tea stains-inspired initiator primer for surface grafting of antifouling and antimicrobial polymer brush coatings.

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    pub_type: 杂志文章


    authors: Pranantyo D,Xu LQ,Neoh KG,Kang ET,Ng YX,Teo SL

    更新日期:2015-03-09 00:00:00

  • Enzymatic synthesis of inulin-containing hydrogels.

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    pub_type: 杂志文章


    authors: Ferreira L,Carvalho R,Gil MH,Dordick JS

    更新日期:2002-03-01 00:00:00

  • A Route to Aliphatic Poly(ester)s with Thiol Pendant Groups: From Monomer Design to Editable Porous Scaffolds.

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    pub_type: 杂志文章


    authors: Fuoco T,Finne-Wistrand A,Pappalardo D

    更新日期:2016-04-11 00:00:00

  • Tensile Reinforcement of Silk Films by the Addition of Telechelic-Type Polyalanine.

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    pub_type: 杂志文章


    authors: Tsuchiya K,Masunaga H,Numata K

    更新日期:2017-03-13 00:00:00

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    pub_type: 杂志文章


    authors: Zinchenko AA,Baigl D,Chen N,Pyshkina O,Endo K,Sergeyev VG,Yoshikawa K

    更新日期:2008-07-01 00:00:00

  • Library of Derivatizable Multiblock Copolymers by Nucleophilic Substitution Polymerization and Targeting Specific Properties.

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    pub_type: 杂志文章


    authors: Nutan B,Kumar A,Jewrajka SK

    更新日期:2020-12-14 00:00:00

  • Pectin-GPTMS-Based Biomaterial: toward a Sustainable Bioprinting of 3D scaffolds for Tissue Engineering Application.

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    pub_type: 杂志文章


    authors: Lapomarda A,De Acutis A,Chiesa I,Fortunato GM,Montemurro F,De Maria C,Mattioli Belmonte M,Gottardi R,Vozzi G

    更新日期:2020-02-10 00:00:00

  • PGSE-NMR and SANS studies of the interaction of model polymer therapeutics with mucin.

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    pub_type: 杂志文章


    authors: Griffiths PC,Occhipinti P,Morris C,Heenan RK,King SM,Gumbleton M

    更新日期:2010-01-11 00:00:00

  • Chromatographic and enzymatic strategies to reveal differences between amidated pectins on a molecular level.

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    pub_type: 杂志文章


    authors: Guillotin SE,Mey N,Ananta E,Boulenguer P,Schols HA,Voragen AG

    更新日期:2006-06-01 00:00:00

  • Transiently Responsive Block Copolymer Micelles Based on N-(2-Hydroxypropyl)methacrylamide Engineered with Hydrolyzable Ethylcarbonate Side Chains.

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    pub_type: 杂志文章


    authors: Kasmi S,Louage B,Nuhn L,Van Driessche A,Van Deun J,Karalic I,Risseeuw M,Van Calenbergh S,Hoogenboom R,De Rycke R,De Wever O,Hennink WE,De Geest BG

    更新日期:2016-01-11 00:00:00

  • Pectin-based injectable biomaterials for bone tissue engineering.

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    pub_type: 杂志文章


    authors: Munarin F,Guerreiro SG,Grellier MA,Tanzi MC,Barbosa MA,Petrini P,Granja PL

    更新日期:2011-03-14 00:00:00

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    pub_type: 杂志文章


    authors: Hoyer B,Bernhardt A,Heinemann S,Stachel I,Meyer M,Gelinsky M

    更新日期:2012-04-09 00:00:00

  • Dielectric relaxation spectroscopy of hydrated and dehydrated silk fibroin cast from aqueous solution.

    abstract::The dynamics of silk protein in the presence and absence of water has been investigated by dielectric relaxation spectroscopy (DRS). The silk fibroin film cast from its water solution contains 4-7 wt % bound water molecules, which can be removed by dehydration at 165 °C. Temperature and frequency scans were performed ...


    pub_type: 杂志文章


    authors: Yu L,Hu X,Kaplan D,Cebe P

    更新日期:2010-10-11 00:00:00

  • Protective effects and mechanisms of G5 PAMAM dendrimers against acute pancreatitis induced by caerulein in mice.

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    pub_type: 杂志文章


    authors: Tang Y,Han Y,Liu L,Shen W,Zhang H,Wang Y,Cui X,Wang Y,Liu G,Qi R

    更新日期:2015-01-12 00:00:00

  • Molecular orientation distributions in a biaxially oriented poly(L-lactic acid) film determined by polarized Raman spectroscopy.

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    pub_type: 杂志文章


    authors: Tanaka M,Young RJ

    更新日期:2006-09-01 00:00:00

  • Poly(N-vinylpyrrolidone)-block-poly(vinyl acetate) as a drug delivery vehicle for hydrophobic drugs.

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    pub_type: 杂志文章


    authors: Bailly N,Thomas M,Klumperman B

    更新日期:2012-12-10 00:00:00

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    abstract::Novel biodegradable poly(carbonate ester)s with photolabile protecting groups were synthesized by ring-opening copolymerization of L-lactide (LA) with 5-methyl-5-(2-nitro-benzoxycarbonyl)-1,3-dioxan-2-one (MNC) with diethyl zinc (Et2Zn) as catalyst. The poly(L-lactide-co-5-methyl-5-carboxyl-1,3-dioxan-2-one) (P(LA-co-...


    pub_type: 杂志文章


    authors: Xie Z,Hu X,Chen X,Sun J,Shi Q,Jing X

    更新日期:2008-01-01 00:00:00

  • Enzymatic synthesis and characterization of novel biodegradable copolymers of 5-benzyloxy-trimethylene carbonate with 1,4-dioxan-2-one.

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    pub_type: 杂志文章


    authors: He F,Jia HL,Liu G,Wang YP,Feng J,Zhuo RX

    更新日期:2006-08-01 00:00:00