Abstract:
:A His-tagged coiled coil stem loop peptide with stable secondary structure was designed and biosynthesized. A series of oligopeptides related to the EBV envelope glycoprotein 350/220 N-terminal nonapeptide as potential CD21 receptor-binding epitopes were engineered into the loop region of the peptide scaffold. It was shown that these peptides had a stable alpha-helical coiled coil structure and assumed a monomeric form in PBS. Biorecognition of the epitopes was studied by immobilizing the epitope-containing peptides on complexed Ni2+-containing surfaces through His-Ni2+ chelation and incubating with purified soluble CD21 receptor or CD21+ cells. The results showed that the potential epitopes bound to CD21 and CD21+ cells at different affinities depending on oligopeptide structures. This approach allows for the evaluation of epitope biorecognizabilities and the selection of optimal oligopeptides among sequences for use as targeting moieties in the design of new lymphoma-targeting polymeric drug carriers.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Tang A,Kopecek Jdoi
10.1021/bm015606+keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
421-31issue
3eissn
1525-7797issn
1526-4602pii
bm015606+journal_volume
3pub_type
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journal_title:Biomacromolecules
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