Abstract:
:Silicatein α exists within the protein filament of silica spicules of the marine sponge Tethya aurantium in a predominantly β-sheet structure. However, it is produced in a soluble form with mixed α-helix/β-sheet structure akin to its cathepsin L homologue. To understand this conformational transition in the context of enzyme catalyzed silica condensation, we used a functional, recombinant silicatein α termed 4SER. In solution, 4SER becomes conformationally unstable at pH 7 and readily unfolds to a soluble β-sheet intermediate, losing the majority of its helical structure. This β-sheet intermediate is present following adsorption of 4SER to a silica surface from solution. 4SER is particularly surface active, forming a near saturated monolayer on SiO2 from low bulk concentrations, without transition to multilayers at high bulk concentrations. The adsorbed intermediate remains stable during silica condensation and drying. We propose that the β-sheet structure for silicatein α in marine sponge spicules represents a stable structural intermediate, formed upon adsorption to the silica surface.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Patwardhan SV,Holt SA,Kelly SM,Kreiner M,Perry CC,van der Walle CFdoi
10.1021/bm100932esubject
Has Abstractpub_date
2010-11-08 00:00:00pages
3126-35issue
11eissn
1525-7797issn
1526-4602journal_volume
11pub_type
杂志文章abstract::PLGA nanoparticles are among the most studied polymer nanoformulations for several drugs against different kinds of malignant diseases, thanks to their in vivo stability and tumor localization exploiting the well-documented "enhanced permeation and retention" (EPR) effect. In this paper, we have developed uniform curc...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3017324
更新日期:2013-03-11 00:00:00
abstract::The effect of solution pH during layer-by-layer assembly of polyelectrolyte multilayer (PEM) coatings on properties relevant to orthopedic implant success was investigated. Bone morphogenetic protein 2 (BMP-2), a potent osteoconductive growth factor, was adsorbed onto the surface of anodized titanium, and PEM coatings...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01730
更新日期:2016-06-13 00:00:00
abstract::The aim of this investigation was to understand and engineer the interactions between endothelial cells and the electrospun (ES) polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) nanofiber surfaces and evaluate their potential for endothelialization. Elastomeric PVDF-HFP samples were electrospun to evaluate th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401825c
更新日期:2014-04-14 00:00:00
abstract::A systematic study is carried out to understand how physical and covalent crosslinks affect the mechanical properties of an eight-arm poly(ethyleneglycol)-based hydrogel. Heparin and heparin-binding peptide are used as a physical crosslinker, and an enzymatically cleavable peptide with a cysteine on each end serves as...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801270k
更新日期:2009-05-11 00:00:00
abstract::Nanofibrous biocomposite scaffolds of type I collagen and nanohydroxyapatite (nanoHA) of varying compositions (wt %) were prepared by electrostatic cospinning. The scaffolds were characterized for structure and morphology by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), atomic fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060879w
更新日期:2007-02-01 00:00:00
abstract::Antibacterial nanodevices could bring coatings of plastic materials and wound dressings a big step forward if the release of the antibacterial agents could be triggered by the presence of the bacteria themselves. Here, we show that novel hyaluronic acid (HA)-based nanocapsules containing the antimicrobial agent polyhe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm302003m
更新日期:2013-04-08 00:00:00
abstract::Bioresponsive poly(amidoamine)s (PAA)s are currently under development as endosomolytic polymers for intracellular delivery of proteins and genes. Here for the first time, small-angle neutron scattering (SANS) is used to systematically investigate the pH-dependent conformational change of an endosomolytic polymer, the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049936g
更新日期:2004-07-01 00:00:00
abstract::Ruthenium(II) tris(bipyridine)-centered poly(ethylenimine) (Ru PEI) was synthesized via acid hydrolysis of Ru tris(bipyridine)-centered poly(2-ethyl-2-oxazoline) (Ru PEOX), and the luminescence, DNA entrapment, and transfection efficiencies were evaluated. Emission maxima for Ru PEI samples are red-shifted compared to...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700481h
更新日期:2007-09-01 00:00:00
abstract::Spider silk has been extensively studied for its outstanding mechanical properties. Partial intermediate and C-terminal sequences of different spider silk proteins have been determined, and during the past decade also N-terminal domains have been characterized. However, only some of these N-terminal domains have been ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060693x
更新日期:2006-11-01 00:00:00
abstract::Positive strand RNA viruses replicate in specialized niches called membranous web within the cytoplasm of host cells. These virus replication organelles sequester viral proteins, RNA, and a variety of host factors within a fluid, amorphous matrix of clusters of endoplasmic reticulum (ER) derived vesicles. They are tho...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00081
更新日期:2019-04-08 00:00:00
abstract::The ability of microbally produced poly(gamma,d-glutamic acid) to form stable polyelectrolyte-opposite charged surfactant complexes was investigated. A sonicated sample of polyacid with a molecular weight about 10(5) Da and a content of d enantiomer higher than 90% was used in this study. Nearly stoichiometric complex...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034267c
更新日期:2004-01-01 00:00:00
abstract::Sulfate cellulose nanocrystal (CNC) dispersions always present specific self-assembled cholesteric mesophases which is easily affected by the inherent properties of particle size, surface charge, and repulsion or affinity interaction, and external field force generated from ionic potential of added electrolytes, magne...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00927
更新日期:2017-10-09 00:00:00
abstract::Synthetic lethality is a molecular-targeted therapy for selective killing of cancer cells. We exploited a lethal interaction between superoxide dismutase 1 inhibition and Bloom syndrome gene product (BLM) defect for the treatment of colorectal cancer (CRC) cells (HCT 116) with a customized lung cancer screen-1-loaded ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01607
更新日期:2018-03-12 00:00:00
abstract::A strategy to light-trigger ionic cross-linking of alginates by incorporating a photosensitive Ca2+ cage (nitr-T) is presented. Upon irradiation, free Ca2+ was released, and this caused gelation of the alginate solution. Addition of Ca2+ "on-demand" allowed us to obtain homogeneous alginate (ALG) gels using concentrat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400022h
更新日期:2013-05-13 00:00:00
abstract::Smart nanocarriers for the transport of drugs to tumor cells are nowadays of great interest for treating cancer. The use of enzymatic stimuli to cleave peptide-based drug nanocapsules for the selective release of nanocapsule cargo in close proximity to tumor cells opens new possibilities in cancer research. In the pre...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00240
更新日期:2020-04-13 00:00:00
abstract::Thermoresponsive oligo(ethylene glycol)-based copolymers were investigated for trypsin conjugation. These copolymers have been synthesized by atom transfer radical polymerization of 2-(2-methoxyethoxy)ethyl methacrylate (MEO(2)MA) with oligo(ethylene glycol) methyl ether methacrylate (OEGMA(475), M(n) = 475 g.mol(-1))...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1005036
更新日期:2010-08-09 00:00:00
abstract::Recently, some attempts have been made to enhance the gas barrier properties of semicrystalline polymers by precisely controlling the arrangement of their impermeable crystalline lamellae. However, it is still a great challenge to achieve regular arrangement of the lamellae along the direction perpendicular to the gas...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500167u
更新日期:2014-04-14 00:00:00
abstract::A one-pot synthetic methodology for fabricating stimuli-responsive hemicellulose-based hydrogels was developed that consists of the in situ formation of magnetic iron oxide (Fe3O4) nanoparticles during the covalent cross-linking of O-acetyl-galactoglucomannan (AcGGM). The Fe3O4 nanoparticle content controlled the ther...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00801
更新日期:2015-08-10 00:00:00
abstract::New therapeutic strategies for the treatment of neoplastic pathologies and, in particular, metastasis processes are based on the inhibitory effect of angiogenic processes. The present article deals with the design, preparation, and application of new "polymer drugs" with a clear inhibitory effect of the activation of ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100223d
更新日期:2010-07-12 00:00:00
abstract::A polyethylene glycol (PEG) retinylamine (Ret-NH2) conjugate PEG-GFL-NH-Ret with a glycine-phenylalanine-leucine (GFL) spacer was synthesized for controlled oral delivery of Ret-NH2 to treat retinal degenerative diseases, including Stargardt disease (STGD) and age-related macular degeneration (AMD). The peptide spacer...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501352s
更新日期:2014-12-08 00:00:00
abstract::Escherichia coli serogroup O5 comprises two different subgroups (O5ab and O5ac), which are indiscernible from the point of view of standard immunological serotyping. The structural similarities between the O-antigen polysaccharides (PSs) of these two strains are remarkable, with the only difference being the glycosidi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400354y
更新日期:2013-07-08 00:00:00
abstract::This study investigated the impact of the protein adsorption mechanism(s) on the efficiency of thermally controlled cell adhesion and release from poly(N-isopropyl acrylamide) brushes. Large format polymer gradients were used to screen for grafting densities and substrate chemistries that alter both cell adhesion at 3...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301390q
更新日期:2013-01-14 00:00:00
abstract::Dextran surface grafting density was systematically varied via a two-step process involving SiO(2) amination by aminopropyltriethoxy silane (APTES) followed by oxidized dextran (M(w) = 110 kDa) chemisorption. Dextran oxidation kinetics with sodium metaperiodate (NaIO(4)) were quantified by (1)H NMR and pH measurements...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050601o
更新日期:2006-02-01 00:00:00
abstract::Enzymatic adsorption and degradation of three types of aliphatic polyester films and two types of polyolefin films by the extracellular PHB depolymerase from Alcaligenes faecalis T1 have been studied by using a quartz crystal microbalance (QCM) technique. Hydrolysis of poly[(R)-3-hydroxybutyrate] was quantitatively fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0000844
更新日期:2001-04-01 00:00:00
abstract::The interaction of polysaccharides with water has a critical impact on their biological function as well as their technological applications. We performed ellipsometry experiments at different relative humidities (RH) to measure the equilibrium swelling of ultrathin films of different polysaccharides: native and modif...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01098
更新日期:2020-12-14 00:00:00
abstract::Tissue engineering approaches need biomaterials with suitable properties to provide an appropriate environment for cell attachment and growth. The performance of these biomaterials can be greatly enhanced through the incorporation of bioactive agents. For this reason, we developed chitosan films with cell-attachment a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701049g
更新日期:2008-02-01 00:00:00
abstract::Immobilizing zwitterionic molecules on material surfaces has been a promising strategy for creating antifouling surfaces. Herein, we show the ability to surface derivatize an allyl-ether-functionalized thermoplastic polyurethane (TPU) with a zwitterionic thiol in a radically induced thiol-ene reaction. The thermoplast...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00456
更新日期:2020-07-13 00:00:00
abstract::The unsatisfied results of cancer therapy are caused by many issues and metastasis of cancer cells is one of the major challenge. It has been reported that inhibiting the SDF1/CXCR4 interaction can significantly reduce the metastasis of breast cancer cells to regional lymph nodes and lung. Herein, a nanogel system equ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00208
更新日期:2017-06-12 00:00:00
abstract::We have found that dialysis of 5 mg/mL collagen solution into the phosphate solution with a pH of 7.1 and an ionic strength of 151 mM [corrected] at 25 °C results in a collagen gel with a birefringence and tubular pores aligned parallel to the growth direction of the gel. The time course of averaged diameter of tubula...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200869p
更新日期:2012-01-09 00:00:00
abstract::To evaluate the antidiarrheal effect of ginseng, the active principals of ginseng were studied in vitro model of rotavirus infection, the leading cause of severe diarrhea. Two pectic polysaccharides, named as GP50-dHR (56.0 kDa) and GP50-eHR (77.0 kDa), were purified from hot water extract of ginseng by bioassay-linke...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100397p
更新日期:2010-08-09 00:00:00