Abstract:
:The effect of solution pH during layer-by-layer assembly of polyelectrolyte multilayer (PEM) coatings on properties relevant to orthopedic implant success was investigated. Bone morphogenetic protein 2 (BMP-2), a potent osteoconductive growth factor, was adsorbed onto the surface of anodized titanium, and PEM coatings prepared from solutions of poly-l-histidine and poly(methacrylic acid) were built on top of the BMP-2. High levels of BMP-2 released over several months were achieved. Approximately 2 μg/cm(2) of BMP-2 were initially adsorbed on the anodized titanium and a pH-dependent release behavior was observed, with more stable coatings assembled at pH = 6-7. Three different diffusion regimes could be determined from the release profiles: an initial burst release, a sustained release regime, and a depletion regime. BMP-2 was shown to maintain bioactivity after release from a PEM and the presence of a PEM was shown to preserve BMP-2 structure. No visible change was observed in surface roughness as the assembly pH was varied, whereas the surface energy decreased for samples prepared at more basic pH. These results indicate that the initial BMP-2 layer affects PEM surface structure, but not the functional groups exposed on the surface.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Salvi C,Lyu X,Peterson AMdoi
10.1021/acs.biomac.5b01730subject
Has Abstractpub_date
2016-06-13 00:00:00pages
1949-58issue
6eissn
1525-7797issn
1526-4602journal_volume
17pub_type
杂志文章abstract::Articular cartilage defects, caused by trauma, osteoarthritis, or other diseases, always lead to severe joint pain and joint dysfunction. Without access to progenitor cells and the supply of blood and nutrients, the impaired articular cartilage would be short of the capability to self-repair. Although the present clin...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/acs.biomac.9b00043
更新日期:2019-04-08 00:00:00
abstract::Poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are biopolyesters reported to provide favorable microenvironments for cell culture and possess potential for tissue engineering applications. Both biopolymers have been investigated for applications in a variety of medical scenarios,...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1007579
更新日期:2010-10-11 00:00:00
abstract::The oxidation-responsive behaviors of OEGylated poly-L-cysteine homopolypeptides, that is, poly(L-EG(x)MA-C)n, were investigated. These poly-L-cysteine derivatives adopted mixed conformation in water, in which the β-sheet accounted for a significant proportion. Upon oxidation, the thioethers in polypeptide side chains...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm5000554
更新日期:2014-03-10 00:00:00
abstract::Developing new protein-based materials with a programmable assembly is of great scientific interest and technological importance. Inspired by nature's use of post-translational modifications (PTMs) to control the function and location of proteins, we have leveraged lipidation-the PTM of proteins with lipids-to synthes...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01380
更新日期:2020-02-10 00:00:00
abstract::The islet amyloid polypeptide (IAPP) is a regulatory peptide that can aggregate into fibrillar structures associated with type 2 diabetes. In this study, the IAPP21-27 segment was modified with a biotin linker at the N-terminus (Btn-GNNFGAIL) to immobilize peptide fibrils on streptavidin-coated surfaces. Key residues ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01466
更新日期:2020-02-10 00:00:00
abstract::Angiogenesis has long been a desired therapeutic approach to improve clinical outcomes of conditions typified by ischemia. Vascular endothelial growth factor (VEGF) has demonstrated the ability to generate new blood vessels in vivo, but trials using intravenous delivery have not yet produced clinical success. Localize...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm061211k
更新日期:2007-05-01 00:00:00
abstract::Homocystinuria due to loss of cystathionine beta-synthase (CBS) causes accumulation of homocysteine and depletion of cysteine. Current treatments are suboptimal, and thus the development of an enzyme replacement therapy based on PEGylated human truncated CBS (PEG-CBS) has been initiated. Attenuation of potency was obs...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00154
更新日期:2017-06-12 00:00:00
abstract::Hemicellulose-based hydrogels were prepared by radical polymerization of 2-hydroxyethyl methacrylate or poly(ethylene glycol) dimethacrylate with oligomeric hydrosoluble hemicellulose modified with well-defined amounts of methacrylic functions. The polymerization reaction was carried out in water at 40 degrees C using...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049515z
更新日期:2005-03-01 00:00:00
abstract::Lignin-carbohydrate complexes (LCCs) were prepared in quantitative yield from spruce wood and from the corresponding kraft and oxygen-delignified pulps and were separated into different fractions on the basis of their carbohydrate composition. To obtain an understanding of the differences in lignin structure and react...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm058014q
更新日期:2005-11-01 00:00:00
abstract::Light-harvesting antenna core (LH1-RC) complexes isolated from Rhodoseudomonas palustris were self-assembled on a gold electrode modified with self-assembled monolayers (SAMs) of the alkanethiols NH2(CH2)nSH, n = 2, 6, 8, 11; HOOC(CH2)7SH; and CH3(CH2)7SH, respectively. Adsorption of the LH1-RC complexes on the SAMs d...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070352z
更新日期:2007-08-01 00:00:00
abstract::Injectable gels that support load are desirable for restoring the mechanical properties of degenerated load-bearing tissue. As these gels become increasingly sophisticated, the need to remotely image them and monitor their swelling increases. However, imaging such gels and monitoring their swelling using noninvasive m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00463
更新日期:2019-07-08 00:00:00
abstract::Recent developments in ionic liquid electrolytes for cellulose or biomass dissolution has also allowed for high-resolution 1H and 13C NMR on very high molecular weight cellulose. This permits the development of advanced liquid-state quantitative NMR methods for characterization of unsubstituted and low degree of subst...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00295
更新日期:2018-07-09 00:00:00
abstract::Poly((N-vinylpyrrolidone)-block-poly(vinyl acetate)) (PVP-b-PVAc) block copolymers of varying molecular weight and hydrophobic block lengths were synthesized via controlled radical polymerization and investigated as carriers for the solubilization of highly hydrophobic riminophenazine compounds. These compounds have r...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301410d
更新日期:2012-12-10 00:00:00
abstract::Nucleic acids have been engineered to participate in a wide variety of tasks. Among them, the enzyme-free amplification modes, enzyme-free DNA circuits (EFDCs), and hybridization chain reactions (HCRs) have been widely applied in a series of studies of bioanalysis. We demonstrated here an ultrasensitive hairpin probe-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00959
更新日期:2015-11-09 00:00:00
abstract::Cell cultivation on the surface of a novel poly(N-isopropylacrylamide) (PNIPA) hydrogel (N-NC gel), consisting of a specific type of organic (PNIPA)/inorganic (clay) network, was studied using three cell types; HepG2 human hepatoma cells, human dermal fibroblasts, and human umbilical vein endothelial cells. For the fi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060549b
更新日期:2006-11-01 00:00:00
abstract::We present a synthetic approach toward soft, glycooligomer-functionalized microgel particles mimicking carbohydrate presenting cell surfaces and analyze their specific binding to a model lectin (Concanavalin A, ConA). Focusing on multivalent presentation, a series of sequence-controlled glycooligomers with varying spa...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00790
更新日期:2018-08-13 00:00:00
abstract::A variety of important biological events are mediated by the multivalent interaction between relevant oligosaccharides and multiple saccharide receptors on lectins, toxins, and cell surfaces; a variety of glycopolymeric materials have therefore been investigated in studies aimed at manipulating these events. The synth...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050672n
更新日期:2006-02-01 00:00:00
abstract::Structures of three pectic arabinogalactans, one from Vernonia kotschyana (Vk2a) and two from Cochlospermum tinctorium (Ct50A1 and Ct50A2), and their complement fixation and induction of B cell proliferation in vitro were compared. The polysaccharide Vk2a expressed potent biological activity in both assays compared wi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050355g
更新日期:2006-01-01 00:00:00
abstract::The interaction between positively charged poly(amido amine) (PAMAM) dendrimers of generation 4 and DNA has been investigated for two DNA lengths; 2000 basepairs (bp; L = 680 nm) and 4331 bp (L = 1472.5 nm) using a theoretical model by Schiessel for a semiflexible polyelectrolyte and hard spheres. The model was modifi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9000662
更新日期:2009-07-13 00:00:00
abstract::In the present contribution, two-dimensional ATR-FTIR spectroscopy was used to study the diffusion of water in poly(epsilon-caprolactone) (PCL). In the spectral region of the nu(OH) stretching vibration of water, four absorption bands (3635, 3560, 3411, and 3220 cm(-1)) can be identified. The higher wavenumber band pa...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0340624
更新日期:2003-07-01 00:00:00
abstract::Water soluble polymer anticancer conjugates can improve the pharmacokinetics of covalently bound drugs by limiting cellular uptake to the endocytic route, thus prolonging plasma circulation time and consequently facilitating tumor targeting by the enhanced permeability and retention (EPR) effect. Many of the first gen...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049381p
更新日期:2005-03-01 00:00:00
abstract::Peptide ligation is an indispensable step in the chemical synthesis of target peptides and proteins that are difficult to synthesize at once by a solid-phase synthesis. The ligation reaction is generally conducted with two peptide fragments at a high aqueous concentration to increase the reaction rate; however, this o...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01655
更新日期:2019-03-11 00:00:00
abstract::The copolymer of methyl methacrylate (MMA) and 2-[2',3',5'-triiodobenzoyl]oxoethyl methacrylate (1), ratio 3:1 (mass:mass), was prepared via a free-radical polymerization in bulk. The copolymer (M(w) = 97.8 kD and M(n) = 41.5 kD) was dissolved in chloroform and subsequently transformed into beads with a diameter in th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm030002m
更新日期:2003-05-01 00:00:00
abstract::The dynamics of silk protein in the presence and absence of water has been investigated by dielectric relaxation spectroscopy (DRS). The silk fibroin film cast from its water solution contains 4-7 wt % bound water molecules, which can be removed by dehydration at 165 °C. Temperature and frequency scans were performed ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm1008316
更新日期:2010-10-11 00:00:00
abstract::Protein transduction domains (PTDs) that readily transverse cellular membranes are of great interest and are attractive tools for the intracellular delivery of bioactive molecules. Learning to program synthetic polymers and oligomers with the appropriate chemical information to capture adequately the biological activi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200694u
更新日期:2011-08-08 00:00:00
abstract::In this work, biodegradable branched polycationic polymers were synthesized by Michael addition polymerization from different amine monomers and the triacrylate monomer trimethylolpropane triacrylate. The polymers varied in the number of amines that dissociate in different pH ranges, which are considered to be benefic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901147k
更新日期:2010-03-08 00:00:00
abstract::Water-soluble conjugated polymers (WCPs) composed of a hydrophobic polythiophene main chain with hydrophilic tertiary amine side-chains can directly self-assemble into sphere-like nano-objects in an aqueous solution due to phase separation between the hydrophilic and hydrophobic segments of the polymeric structure. Du...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01336
更新日期:2020-12-14 00:00:00
abstract::Super-resolution fluorescence microscopy, specifically stochastic reconstruction microscopy (STORM), and atomic force microscopy (AFM) were used to image the self-assembly processes of the peptide surfactant I3K. The peptide surfactants self-assembled into giant helical fibrils with diameters between 5 and 10 nm with ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00465
更新日期:2017-11-13 00:00:00
abstract::Biocompatible photoresponsive materials are of interest for targeted drug delivery, tissue engineering, 2D and 3D protein patterning, and other biomedical applications. We prepared light degradable hydrogels using a natural alginate polysaccharide cross-linked with iron(III) cations. The "hard" iron(III) cations used ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm300707a
更新日期:2012-08-13 00:00:00
abstract::Controlled reeling is a powerful tool to investigate the details of silk processing. However, consistent forced reeling of silkworms is hindered by the significant degree of behaviorally induced variation caused by the animal. This paper proposes silkworm paralysis as a novel method to control the animal and thus in v...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401013k
更新日期:2013-10-14 00:00:00