Abstract:
:The aim of this investigation was to understand and engineer the interactions between endothelial cells and the electrospun (ES) polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) nanofiber surfaces and evaluate their potential for endothelialization. Elastomeric PVDF-HFP samples were electrospun to evaluate their potential use as small diameter artificial vascular graft scaffold (SDAVG) and compared with solvent cast (SC) PVDF-HFP films. We examined the consequences of fibrinogen adsorption onto the ES and SC samples for endothelialisation. Bone marrow derived endothelial cells (BMEC) of human origin were incubated with the test and control samples and their attachment, proliferation, and viability were examined. The nature of interaction of fibrinogen with SC and ES samples was investigated in detail using ELISA, XPS, and FTIR techniques. The pristine SC and ES PVDF-HFP samples displayed hydrophobic and ultrahydrophobic behavior and accordingly, exhibited minimal BMEC growth. Fibrinogen adsorbed SC samples did not significantly enhance endothelial cell binding or proliferation. In contrast, the fibrinogen adsorbed electrospun surfaces showed a clear ability to modulate endothelial cell behavior. This system also represents an ideal model system that enables us to understand the natural interaction between cells and their extracellular environment. The research reported shows potential of ES surfaces for artificial vascular graft applications.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Ahmed F,Dutta NK,Zannettino A,Vandyke K,Choudhury NRdoi
10.1021/bm401825csubject
Has Abstractpub_date
2014-04-14 00:00:00pages
1276-87issue
4eissn
1525-7797issn
1526-4602journal_volume
15pub_type
杂志文章abstract::Glycosaminoglycans (GAGs) and glycoproteins are vital components of the extracellular matrix, directing cell proliferation, differentiation, and migration and tissue homeostasis. Here, we demonstrate supramolecular GAG-like glycopeptide nanofibers mimicking bioactive functions of natural hyaluronic acid molecules. Sel...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01669
更新日期:2016-02-08 00:00:00
abstract::The controlled delivery of growth factors is a very challenging task because many different issues have to be addressed to develop the best suited system. A wide range of approaches have been employed for the controlled delivery of growth factors by hydrogels. Direct loading, electrostatic interaction, covalent bindin...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm801103c
更新日期:2009-01-12 00:00:00
abstract::A dual-sensitive polymeric drug conjugate (HA-SS-MP) was synthesized by conjugating hydrophobic 6-mercaptopurine (MP) to thiolated hyaluronic acid (HA) as the carrier and ligand to deliver doxorubicin (Dox) to parental colon cancer and colon cancer stem cells. Because of the amphiphilic nature of HA-SS-MP, it was self...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00856
更新日期:2018-09-10 00:00:00
abstract::We report the engineering of intracellular redox-responsive nanoporous poly(ethylene glycol)-poly(l-lysine) particles (NPEG-PLLs). The obtained particles exhibit no toxicity while maintaining the capability to deliver a small interfering RNA sequence (siRNA) targeting the anti-apoptotic factor, survivin, in prostate c...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00562
更新日期:2015-07-13 00:00:00
abstract::Hierarchical nanocomposite films with Pd-PdO nanoparticles anchored uniformly on the inner surface of TiO2 nanotubes were achieved through a stepwise bioredox/artificial oxygenation approach by using the natural eggshell membrane (ESM) as a template. The Pd content ratio of Pd-PdO loading could be arbitrarily varied f...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700993m
更新日期:2008-02-01 00:00:00
abstract::Incorporating charged amino acid side chains in polypeptide polymer backbones to improve solubility usually leads to reduced secondary structuring. Here we show that highly water soluble (>15 mg.mL-1) β-sheets can be obtained via nucleotide monophosphate grafting onto simple poly(γ-propargyl- L-glutamate) backbone. Th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01084
更新日期:2018-10-08 00:00:00
abstract::The present study demonstrates the controlled synthesis and biological potential of poly( N-acryloyl-l-methionine methyl sulfonium salt)s (poly(A-Met(S+)-OH)s), which mimic dimethylsulfoniopropionate (DMSP), a compound produced by marine algae to protect their proteins. The novel sulfonium-containing zwitterionic poly...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01542
更新日期:2019-02-11 00:00:00
abstract::Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. He...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b00823
更新日期:2015-09-14 00:00:00
abstract::Gastric mucin, a high molecular weight glycoprotein, is responsible for providing the gel-forming properties and protective function of the gastric mucus layer. Bulk rheology measurements in the linear viscoelastic regime show that gastric mucin undergoes a pH-dependent sol-gel transition from a viscoelastic solution ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0609691
更新日期:2007-05-01 00:00:00
abstract::Physicochemical properties of a homogeneous series of chitosans with different degrees of acetylation and almost the same degree of polymerization were investigated in an ammonium acetate buffer. Techniques such as interferometry, static light scattering (in batch or coupled on line with a chromatographic system), and...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025724c
更新日期:2003-05-01 00:00:00
abstract::The carboxyl-terminal domain (CTD, residues 146-231) of the HIV-1 capsid (CA) protein plays an important role in the CA-CA dimerization and viral assembly of the human immunodeficiency virus type 1. Disrupting the native conformation of the CA is essential for blocking viral capsid formation and viral replication. Thu...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801151r
更新日期:2009-02-09 00:00:00
abstract::Imbalanced homeostasis and oligomerization of the amyloid-β (Aβ) peptide in the brain are hallmarks of Alzheimer's disease (AD). Microglia and macrophages play a critical role in the etiology of AD either by clearing Aβ from the brain or inducing inflammation. Recent evidence suggests that clearance of Aβ by microglia...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301401b
更新日期:2013-01-14 00:00:00
abstract::The structure of an extracellular polysaccharide (EPS) from Streptococcus thermophilus THS has been determined. A combination of component analysis, methylation analysis and NMR spectroscopy shows that the polysaccharide is composed of pentasaccharide repeating units. Sequential information was obtained by two-dimensi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0496514
更新日期:2005-01-01 00:00:00
abstract::This paper describes the synthesis and cytotoxicity of poly(anhydride esters) that are composed of several salicylate derivatives, including halogenated salicylates, aminosalicylates, salicylsalicylic acid, and thiolsalicylic acid. The incorporation of these nonsteroidal antiinflammatory drugs (NSAIDs) into a biodegra...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049544+
更新日期:2005-01-01 00:00:00
abstract::Electrospinning is a versatile process used to prepare micro- and nano- sized fibers from various polymers dissolved in volatile solvents. In this report, cellulose and cellulose-heparin composite fibers are prepared from nonvolatile room temperature ionic liquid (RTIL) solvents by electrospinning. RTILs are extracted...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050837s
更新日期:2006-02-01 00:00:00
abstract::Polymer therapeutics, including polymeric drugs and polymer-protein conjugates, are clinically established as first-generation nanomedicines. Knowing that the coiled-coil peptide motif is fundamentally important in the regulation of many cellular and pathological processes, the aim of these studies was to examine the ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100843e
更新日期:2011-01-10 00:00:00
abstract::An injectable, heparin-based hydrogel system with the potential to be gelled with cells was developed. First, heparin was modified to have thiol groups by the modification of carboxylic groups of heparin with cysteamine using carbodiimide chemistry. Thiol functionalization of heparin carboxylic groups was controlled f...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0701189
更新日期:2007-06-01 00:00:00
abstract::Although micelles are commonly used for drug delivery purposes, their long-term fate is often unknown due to photobleaching of the fluorescent labels or the use of toxic materials. Here, we present a metal-free, nontoxic, nonbleaching, fluorescent micelle that can address these shortcomings. A simple, yet versatile, p...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01653
更新日期:2016-12-12 00:00:00
abstract::Cellulose and starch nanocrystals obtained from the acid hydrolysis of ramie fibers and waxy maize starch granules, respectively, were subjected to isocyanate-mediated reaction to graft polycaprolactone (PCL) chains with various molecular weights on their surface. Grafted nanoparticles were characterized by X-ray diff...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm8001717
更新日期:2008-07-01 00:00:00
abstract::Herein we present a unique method of using dynamic cross-links, which are dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and dibenzaldhyde capped poly(ethylene oxide) formed the ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b00452
更新日期:2017-07-10 00:00:00
abstract::The mechanical rigidity and degradation rate of hydrogels utilized as cell transplantation vehicles have been regarded as critical factors in new tissue formation. However, conventional approaches to accelerate the degradation rate of gels deteriorate their function as a mechanical support in parallel. We hypothesized...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049879r
更新日期:2004-09-01 00:00:00
abstract::The effect of guluronate oligomers on the barrier properties of mucous matrices was investigated in terms of the mobility of nanoparticles in mucous matrices by fluorescence recovery after photobleaching (FRAP), cellular uptake of nanoparticles in mucus secreting cells (HT29-MTX), and mucin matrix architecture by scan...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500464b
更新日期:2014-06-09 00:00:00
abstract::The intermolecular interactions of lignin with a hydrophilic polymer, poly(vinyl alcohol) (PVA), were studied using thermal analyses and FT-IR spectroscopy of a series of PVA/hardwood kraft lignin blend fibers prepared by thermal extrusion. Although two phases are observed in this blend system, some of the lignin was ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025727p
更新日期:2003-05-01 00:00:00
abstract::N-Alkylated chitosan (NACS) may improve the blood clotting efficiency of chitosan (CS). To study its blood coagulation capability, a series of NACSs with various carbon chain lengths and degrees of substitution (DS) of alkyl groups were synthesized and characterized by FTIR, NMR, elemental analysis, and X-ray diffract...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.7b01492
更新日期:2018-03-12 00:00:00
abstract::In this work, biodegradable branched polycationic polymers were synthesized by Michael addition polymerization from different amine monomers and the triacrylate monomer trimethylolpropane triacrylate. The polymers varied in the number of amines that dissociate in different pH ranges, which are considered to be benefic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901147k
更新日期:2010-03-08 00:00:00
abstract::Developing green and nontoxic biomaterials, derived from renewable sources and processable through 3D bioprinting technologies, is an emerging challenge of sustainable tissue engineering. Here, pectin from citrus peels was cross-linked for the first time with (3-glycidyloxypropyl)trimethoxysilane (GPTMS) through a one...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01332
更新日期:2020-02-10 00:00:00
abstract::Photo-crosslinkable dendritic macromolecules are attractive materials for the preparation of cartilage tissue engineering scaffolds that may be optimized for in situ formation of hydrated, mechanically stable, and well-integrated hydrogel scaffolds supporting chondrocytes and chondrogenesis. We designed and synthesize...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050663e
更新日期:2006-01-01 00:00:00
abstract::Immobilizing zwitterionic molecules on material surfaces has been a promising strategy for creating antifouling surfaces. Herein, we show the ability to surface derivatize an allyl-ether-functionalized thermoplastic polyurethane (TPU) with a zwitterionic thiol in a radically induced thiol-ene reaction. The thermoplast...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00456
更新日期:2020-07-13 00:00:00
abstract::Native hemicellulose lacks many of the properties that make fossil fuel-based polymers excellent for use in today's industrial products and processes. The mechanical and thermal properties of the hemicellulose can, however, be modified, and its processability increased. We functionalized galactoglucomannan to lower it...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01730
更新日期:2020-05-11 00:00:00
abstract::Injectable gels that support load are desirable for restoring the mechanical properties of degenerated load-bearing tissue. As these gels become increasingly sophisticated, the need to remotely image them and monitor their swelling increases. However, imaging such gels and monitoring their swelling using noninvasive m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b00463
更新日期:2019-07-08 00:00:00