Exploration of Blood Coagulation of N-Alkyl Chitosan Nanofiber Membrane in Vitro.

Abstract:

:N-Alkylated chitosan (NACS) may improve the blood clotting efficiency of chitosan (CS). To study its blood coagulation capability, a series of NACSs with various carbon chain lengths and degrees of substitution (DS) of alkyl groups were synthesized and characterized by FTIR, NMR, elemental analysis, and X-ray diffraction (XRD). The corresponding NACS nanofiber membranes (NACS-NM) were subsequently fabricated by electronic spinning technique. SEM, XRD, DSC, surface area, porosity, contact angle, blood absorption, and mechanical properties were used to characterize the CS-NM/NACS-NM. Moreover, cytotoxicity, coagulation, activated partial thromboplastin time, plasma prothrombin time, thrombin time, and platelet aggregation tests were performed to evaluate the biocompatibility and blood coagulation properties of NACS-NM. The results showed that NACS-NM was not cytotoxic. NACS-NM with DS of 19.25% for N-hexane CS (CS6b), 17.87% for N-dodecane CS (CS12b), and 8.97% for N-octadecane CS (CS18a) exhibited good blood clotting performance. Moreover, NACS-NMs favored the activation of coagulation factors and platelets. In addition, intracellular Ca2+ was not related to platelet activation. The above results suggested that NACS-NM would be an effective hemostatic agent.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Wang X,Guan J,Zhuang X,Li Z,Huang S,Yang J,Liu C,Li F,Tian F,Wu J,Shu Z

doi

10.1021/acs.biomac.7b01492

subject

Has Abstract

pub_date

2018-03-12 00:00:00

pages

731-739

issue

3

eissn

1525-7797

issn

1526-4602

journal_volume

19

pub_type

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