Differentiation of osteoblasts on pectin-coated titanium.

Abstract:

:The gold standard for implant metals is titanium, and coatings such as collagen-I, RGD-peptide, chondroitin sulfate, and calcium phosphate have been used to modify its biocompatibility. We investigated how titanium coated with pectins, adaptable bioactive plant polysaccharides with anti-inflammatory effects, supports osteoblast differentiation. MC3T3-E1 cells, primary murine osteoblasts, and human mesenchymal cells (hMC) were cultured on titanium coated with rhamnogalacturonan-rich modified hairy regions (MHR-A and MHR-B) of apple pectin. Alkaline phosphatase (ALP) expression and activity, calcium deposition, and cell spreading were investigated. MHR-B, but not MHR-A, supports osteoblast differentiation. The MHR-A surface was not mineralized, but on MHR-B, the average mineralized area was 14.0% with MC3T3-E1 cells and 26.6% with primary osteoblasts. The ALP activity of hMCs on MHR-A was 58.3% at day 7 and 9.3% from that of MHR-B at day 10. These data indicate that modified pectin nanocoatings may enhance the biocompatibility of bone and dental implants.

journal_name

Biomacromolecules

journal_title

Biomacromolecules

authors

Kokkonen H,Cassinelli C,Verhoef R,Morra M,Schols HA,Tuukkanen J

doi

10.1021/bm800356b

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

2369-76

issue

9

eissn

1525-7797

issn

1526-4602

journal_volume

9

pub_type

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