Abstract：
:Cellular model systems are essential platforms used across multiple research fields for exploring the fundaments of biology and biochemistry. Here, we present giant plasma membrane vesicles (GPMVs) as a platform of cell-like compartments that will facilitate the study of particles within a biorelevant environment and promote their further development. We studied how cellularly taken up nanoparticles (NPs) can be transferred into formed GPMVs and which are the molecular factors that play a role in successful transfer (size, concentration, and surface charge along with 3 different cell lines: HepG2, HeLa, and Caco-2). We observed that polystyrene (PS) carboxylated NPs with a size of 40 and 100 nm were successfully and efficiently transferred to GPMVs derived from all cell lines. We then investigated the distribution of NPs inside formed GPMVs and established the average number of NPs/GPMVs and the percentage of all GPMVs with NPs in their cavity. We pave the way for GPMV usage as superior cell-like mimics in medically relevant applications.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Zartner L,Garni M,Craciun I,Einfalt T,Palivan CGdoi
10.1021/acs.biomac.0c00624subject
Has Abstractpub_date
2021-01-11 00:00:00pages
106-115issue
1eissn
1525-7797issn
1526-4602journal_volume
22pub_type
杂志文章abstract：:The remarkable diversity of the self-assembly behavior of PEG-peptides is reviewed, including self-assemblies formed by PEG-peptides with β-sheet and α-helical (coiled-coil) peptide sequences. The modes of self-assembly in solution and in the solid state are discussed. Additionally, applications in bionanotechnology a...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm500246w
更新日期：2014-05-12 00:00:00
abstract：:The interaction between DNA and different types of amino acid-based cationic surfactants was investigated. Particular attention was directed to determine the extent of influence of surfactant head-group geometry toward tuning the interaction behavior of these surfactants with DNA. An overview is obtained by gel retard...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm8000765
更新日期：2008-07-01 00:00:00
abstract：:The need for thermosets from renewable resources is continuously increasing to find eco-friendly alternatives to petroleum-derived materials. Products obtained from biomass have shown to play an important role in this challenge. Here, we present the structural characterization of new biobased thermosets made of humins...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01248
更新日期：2020-02-10 00:00:00
abstract：:Cancer cells have a large amount of ROS (reactive oxygen species) because of disturbed ROS homeostasis. Cancer cells therefore undertake redox adaptation to drive proliferation in tumor environments and even survive during anticancer treatment by upregulating endogenous antioxidants. As one of antioxidant defense syst...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01802
更新日期：2019-02-11 00:00:00
abstract：:Horseradish peroxidase-catalyzed polymerization of cardanol in aqueous organic solvent was investigated in the presence of a redox mediator. Cardanol is a phenol derivative from a renewable resource mainly having a C15 unsaturated hydrocarbon chain with mostly 1-3 double bonds at a meta position. Unlike soybean peroxi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034325u
更新日期：2004-01-01 00:00:00
abstract：:Silk fibroin (SF) protein, produced by silkworm Bombyx mori, is a promising biomaterial, while sophorolipid (SL) is an amphiphilic functional biosurfactant synthesized by nonpathogenic yeast Candida bombicola. SL is a mixture of two forms, acidic (ASL) and lactonic (LSL), which when added to SF results in accelerated ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01069
更新日期：2016-10-10 00:00:00
abstract：:Biodegradable aliphatic polyesters such as poly(lactide) and poly(ε-caprolactone), largely used in tissue engineering applications, lack suitable functional groups and biological cues to enable interactions with cells. Because of the ubiquity of thiol groups in the biological environment and the pliability of thiol ch...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00005
更新日期：2016-04-11 00:00:00
abstract：:The synthesis, characterization, and degradation kinetics of three alpha-cyclodextrin (alpha-CD)-poly(ethylene glycol) (PEG) polyrotaxanes with endcaps that were installed using Cu(I)-catalyzed Huisgen cyclization is reported. PEG1500, azidated with azidoacetic acid, was threaded with alpha-CD to form a pseudopolyrota...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0602076
更新日期：2006-09-01 00:00:00
abstract：:The objective of this study is to investigate the influence of point mutations on the structural stability of coiled coil fragments of the human hair intermediate filament by molecular dynamics simulations and free energy calculations. Mutations in the helix termination motif of human hair keratin gene hHb6 seem to be...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049788u
更新日期：2004-11-01 00:00:00
abstract：:The intermolecular interactions of lignin with a hydrophilic polymer, poly(vinyl alcohol) (PVA), were studied using thermal analyses and FT-IR spectroscopy of a series of PVA/hardwood kraft lignin blend fibers prepared by thermal extrusion. Although two phases are observed in this blend system, some of the lignin was ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025727p
更新日期：2003-05-01 00:00:00
abstract：:We examined the antibacterial and hemolytic activities in a series of amphiphilic block and random copolymers of poly(vinyl ether) derivatives prepared by base-assisting living cationic polymerization. Block and random amphiphilic copolymers with similar monomer compositions showed the same level of activity against ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200780r
更新日期：2011-10-10 00:00:00
abstract：:Inflammation plays an important role in symptomatic intervertebral disc degeneration and is associated with the production of neurotrophins in sensitizing innervation into the disc. The use of high molecular weight (HMw) hyaluronic acid (HA) hydrogels offers a potential therapeutic biomaterial for nucleus pulposus (NP...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00168
更新日期：2015-06-08 00:00:00
abstract：:n-Heptyl α-d-mannose (HM) is a nanomolar antagonist of FimH, a virulence factor of E. coli. Herein we report on the construction of multivalent HM-based glycopolymers as potent antiadhesives of type 1 piliated E. coli. We investigate glycopolymer/FimH and glycopolymer/bacteria interactions and show that HM-based glyco...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00413
更新日期：2015-06-08 00:00:00
abstract：:Recently antifreeze proteins (AFP) have been the subject of many structure-function relationship studies regarding their antifreeze activity. Attempts have been made to elucidate the structure-function relationship by various amino acid substitutions, but to our knowledge there has been no successful from first princi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm000004w
更新日期：2000-07-01 00:00:00
abstract：:The controlled delivery of growth factors is a very challenging task because many different issues have to be addressed to develop the best suited system. A wide range of approaches have been employed for the controlled delivery of growth factors by hydrogels. Direct loading, electrostatic interaction, covalent bindin...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm801103c
更新日期：2009-01-12 00:00:00
abstract：:Hydrophilic copolymers with high refractive index and bactericide properties based on quaternary ammonium salts monomers and methacrylates bearing benzothiazole moieties have been developed for application as foldable intraocular lenses. Composition of the systems was adjusted to get materials with optimized flexibili...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9006997
更新日期：2009-11-09 00:00:00
abstract：:Double-network (DN) hydrogels are fabricated from poly(2-ethyl-2-oxazoline) (PEOXA)-peptide conjugates, which can be enzymatically crosslinked in the presence of Sortase A (SA), and physical networks of alginate (Alg), yielding matrices with improved mechanical properties with respect to the corresponding PEOXA and Al...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01266
更新日期：2019-12-09 00:00:00
abstract：:A hyperbranched conjugated polyelectrolyte (HCPE) with a core-shell structure is designed and synthesized via alkyne polycyclotrimerization and click chemistry. The HCPE has an emission maximum at 565 nm with a quantum yield of 12% and a large Stokes shift of 143 nm in water. By virtue of its poly(ethylene glycol) she...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200563a
更新日期：2011-08-08 00:00:00
abstract：:A new mathematical model is presented for the analysis of the substituent distribution in the polymer chain of polysaccharide derivatives. For the first time, the influence of substitution on the reactivity of neighbored monomer units is taken into account. The model was applied to various cellulose and amylose ethers...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm000093c
更新日期：2001-04-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060597w
更新日期：2006-12-01 00:00:00
abstract：:The bioconjugation of proteins and peptides with synthetic polymers is a promising method for tailoring the chemical, biological, and physical properties of both the polymeric and protein-based components. Here, we describe macromolecular assemblies of polyethylene glycol-coiled-coil alternating multiblock polymers gu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900474k
更新日期：2009-10-12 00:00:00
abstract：:N-(3-Aminopropyl)pyrrole was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a pyrrole-alginate conjugate for subsequent use in biosensor applications. The pyrrole-alginate conjugate was quantified by UV spectroscopy at 230 nm, by an HPSEC...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm050339j
更新日期：2005-11-01 00:00:00
abstract：:An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201143c
更新日期：2012-01-09 00:00:00
abstract：:Insulin was loaded into hydrogel microparticles after two hours with loading efficiencies greater than 70% for both poly(methacrylic acid-grafted-ethylene glycol) (P(MAA-g-EG)) and poly(methacrylic acid-grafted-ethylene glycol) functionalized with wheat germ agglutinin (P(MAA-g-EG) WGA). The pH-responsive release resu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm701274p
更新日期：2008-04-01 00:00:00
abstract：:New pH-responsive polymersomes for active anticancer oligonucleotide delivery were prepared from triblock copolymers. The delivery systems were formed by two terminal hydrophilic blocks, PEG and polyglycerolmethacrylate (poly-GMA), and a central weakly basic block, polyimidazole-hexyl methacrylate (poly-ImHeMA), which...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00286
更新日期：2015-07-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301865g
更新日期：2013-03-11 00:00:00
abstract：:Genome-editing technologies hold tremendous potential for treating genetic diseases. However, the efficient and safe delivery of genome-editing elements to the location of interest, and the achievement of specific targeted gene correction without off-target side effect remains a big challenge. In this Perspective, we ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00783
更新日期：2019-09-09 00:00:00
abstract：:Self-assembly in aqueous solution of two oppositely charged globular proteins, hen egg white lysozyme (LYS) and bovine calcium-depleted α-lactalbumin (apo α-LA), was investigated at pH 7.5. The aggregation rate of equimolar mixtures of the two proteins was determined using static and dynamic light scattering as a func...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200264m
更新日期：2011-05-09 00:00:00
abstract：:Two kinds of regioselectively substituted cellulose derivatives, i.e., 6-O-triphenylmethylcellulose (6TC) and 2,3-di-O-acetyl-6-O-triphenylmethylcellulose (2,3Ac6TC), were prepared via cellulose. In these samples, C-6 position hydroxyls in the anhydroglucose units (AGU) along the cellulose chain were selectively subst...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm010069e
更新日期：2001-10-01 00:00:00
abstract：:Hydrogels with pH-sensitive poly(acrylic acid) (PAAc) chains and biodegradable acryloyl-poly(-caprolactone)-2-hydroxylethyl methacrylate (AC-PCL-HEMA) chains were designed and synthesized. The morphology of hydrogel was observed by scanning electron microscopy. The degradation of the hydrogel in the presence of Pseudo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm7010328
更新日期：2008-04-01 00:00:00
