Nonviral Nanoparticles for CRISPR-Based Genome Editing: Is It Just a Simple Adaption of What Have Been Developed for Nucleic Acid Delivery?

abstract：:Two linear alpha-(1,4)-D-glucans substrates, of degrees of polymerization DP approximately 150 and 6000, were exposed to maize starch-branching enzyme IIa (mSBEIIa) in vitro. The resulting branched alpha-glucans and their constituent chains (obtained by debranching) were analyzed by nuclear magnetic resonance (NMR) an...

journal_title：Biomacromolecules

authors： Hernández JM,Gaborieau M,Castignolles P,Gidley MJ,Myers AM,Gilbert RG

更新日期：2008-03-01 00:00:00

abstract：:Spider silk has been extensively studied for its outstanding mechanical properties. Partial intermediate and C-terminal sequences of different spider silk proteins have been determined, and during the past decade also N-terminal domains have been characterized. However, only some of these N-terminal domains have been ...

journal_title：Biomacromolecules

authors： Rising A,Hjälm G,Engström W,Johansson J

更新日期：2006-11-01 00:00:00

abstract：:The ability to direct neurite growth into a close proximity of stimulating elements of a neural prosthesis, such as a retinal or cochlear implant (CI), may enhance device performance and overcome current spatial signal resolution barriers. In this work, spiral ganglion neurons (SGNs), which are the target neurons to b...

journal_title：Biomacromolecules

authors： Tuft BW,Zhang L,Xu L,Hangartner A,Leigh B,Hansen MR,Guymon CA

更新日期：2014-10-13 00:00:00

abstract：:The self-assembly properties of fibrous proteins such as collagen are frequently used to form three-dimensional scaffolds. In this study we investigated the effect of nonuniform alternating and static electric-fields on the self-assembly properties of a dilute solution of vimentin. In the presence of both types of fie...

journal_title：Biomacromolecules

authors： Colbourne TR,Hill IG,Kreplak L

更新日期：2009-07-13 00:00:00

abstract：:Nanofibrous biocomposite scaffolds of type I collagen and nanohydroxyapatite (nanoHA) of varying compositions (wt %) were prepared by electrostatic cospinning. The scaffolds were characterized for structure and morphology by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), atomic fo...

journal_title：Biomacromolecules

authors： Thomas V,Dean DR,Jose MV,Mathew B,Chowdhury S,Vohra YK

更新日期：2007-02-01 00:00:00

abstract：:The present study reports the folic acid (FA) functionalized insulin loaded stable liposomes with improved bioavailability following oral administration. Liposomes were stabilized by alternating coating of negatively charged poly(acrylic acid) (PAA) and positively charged poly(allyl amine) hydrochloride (PAH) over lip...

journal_title：Biomacromolecules

authors： Agrawal AK,Harde H,Thanki K,Jain S

更新日期：2014-01-13 00:00:00

abstract：:Assembly of (bio)polymers into long-range anisotropic nanostructured gels and aerogels is of great interest in advanced material engineering since it enables directional tuning of properties, such as diffusivity, light, heat, and sound propagation, cell proliferation, and mechanical properties. Here we present an appr...

journal_title：Biomacromolecules

authors： Plappert SF,Nedelec JM,Rennhofer H,Lichtenegger HC,Bernstorff S,Liebner FW

更新日期：2018-11-12 00:00:00

abstract：:Electrospun poly(glycolide-co-lactide) (PLA10GA90, LA/GA ratio 10/90) biodegradable nanofiber membranes possessed very high surface area to volume ratios and were completely noncrystalline with a relatively lowered glass transition temperature. These characteristics led to very different structure, morphology, and pro...

journal_title：Biomacromolecules

authors： Zong X,Ran S,Kim KS,Fang D,Hsiao BS,Chu B

更新日期：2003-03-01 00:00:00

abstract：:In a previous study, we have reported chemical synthesis of novel aliphatic poly(butylene succinate-co-cyclic carbonate) P(BS-co-CC)s bearing various functionalizable carbonate building blocks, and this work will continue to present our new studies on their enzymatic degradation and in vitro cell biocompatibility assa...

journal_title：Biomacromolecules

authors： Yang J,Tian W,Li Q,Li Y,Cao A

更新日期：2004-11-01 00:00:00

abstract：:The storage and transport of cells is a fundamental technology which underpins cell biology, biomaterials research, and emerging cell-based therapies. Inspired by antifreeze and ice-binding proteins in extremophiles, macromolecular (polymer) cryoprotectants are emerging as exciting biomaterials to enable the reduction...

journal_title：Biomacromolecules

authors： Murray KA,Gibson MI

更新日期：2020-07-13 00:00:00

abstract：:Inspired by tea stains, plant polyphenolic tannic acid (TA) was beneficially employed as the primer anchor for functional polymer brushes. The brominated TA (TABr) initiator primer was synthesized by partial modification of TA with alkyl bromide functionalities. TABr with trihydroxyphenyl moieties can readily anchor o...

journal_title：Biomacromolecules

authors： Pranantyo D,Xu LQ,Neoh KG,Kang ET,Ng YX,Teo SL

更新日期：2015-03-09 00:00:00

abstract：:A one-pot synthetic methodology for fabricating stimuli-responsive hemicellulose-based hydrogels was developed that consists of the in situ formation of magnetic iron oxide (Fe3O4) nanoparticles during the covalent cross-linking of O-acetyl-galactoglucomannan (AcGGM). The Fe3O4 nanoparticle content controlled the ther...

journal_title：Biomacromolecules

authors： Zhao W,Odelius K,Edlund U,Zhao C,Albertsson AC

更新日期：2015-08-10 00:00:00

abstract：:Small-angle X-ray scattering (SAXS) experiments with in situ dehydration have been performed on small bundles of flax fibers. Analysis indicated that the structure of the cellulose microfibrils is consistent with a cross section of approximately 10 x 50 A2. The microfibril misalignment was Gaussian with a full width a...

journal_title：Biomacromolecules

authors： Astley OM,Donald AM

更新日期：2001-10-01 00:00:00

abstract：:Mammalian synovial joints are extremely efficient lubrication systems reaching friction coefficient μ as low as 0.001 at high pressures (up to 100 atm) and shear rates (up to 10(6) to 10(7) Hz); however, despite much previous work, the exact mechanism responsible for this behavior is still unknown. In this work, we st...

journal_title：Biomacromolecules

authors： Seror J,Merkher Y,Kampf N,Collinson L,Day AJ,Maroudas A,Klein J

更新日期：2011-10-10 00:00:00

abstract：:The present study focuses on the formation of microcapsules containing catalytically active L-asparaginase (L-ASNase), a protein drug of high value in antileukemic therapy. We make use of the layer-by-layer (LbL) technique to coat protein-loaded calcium carbonate (CaCO3) particles with two or three poly dextran/poly-L...

journal_title：Biomacromolecules

authors： Karamitros CS,Yashchenok AM,Möhwald H,Skirtach AG,Konrad M

更新日期：2013-12-09 00:00:00

abstract：:A unified kinetic pathway for the enzyme-catalyzed polymerization and degradation of poly(ε-caprolactone) was developed. This model tracks the complete distribution of individual chain lengths, both enzyme-bound and in solution, and successfully predicts monomer conversion and the molecular mass distribution as a func...

journal_title：Biomacromolecules

authors： Johnson PM,Kundu S,Beers KL

更新日期：2011-09-12 00:00:00

abstract：:Steady state shear flow of different types of cellulose (microcrystalline, spruce sulfite and bacterial) dissolved in 1-ethyl-3-methylimidazolium acetate was studied in a large range of concentrations (0-15%) and temperatures (0-100 degrees C). Newtonian flow was recorded for all experimental conditions; these viscosi...

journal_title：Biomacromolecules

authors： Gericke M,Schlufter K,Liebert T,Heinze T,Budtova T

更新日期：2009-05-11 00:00:00

abstract：:Encapsulation of hydrophilic cancer drugs in polymeric nanocapsules was achieved in a one-pot process via the inverse miniemulsion periphery RAFT polymerization (IMEPP) approach. The chosen guest molecule was gemcitabine hydrochloride, which is used as the first-line treatment of pancreatic cancer. The resulting nanoc...

journal_title：Biomacromolecules

authors： Utama RH,Jiang Y,Zetterlund PB,Stenzel MH

更新日期：2015-07-13 00:00:00

abstract：:In this work, we have applied an electrospinning method to control wettability and further hydrophobic modification of a hydrophobic polymer mat of poly(vinylidene fluoride-co-hexafluoropropylene). A correlation between the processing parameters, rheological properties of polymer solutions, and electrospinning ability...

journal_title：Biomacromolecules

authors： Ahmed F,Choudhury NR,Dutta NK,Zannettino A,Knott R

更新日期：2013-11-11 00:00:00

abstract：:External magnetic field is a powerful approach to induce orientational order in originally disordered suspensions of magneto-responsive anisotropic particles. By small angle neutron scattering and optical birefringence measurement technology, we investigated the effect of magnetic field on the spatial ordering of hybr...

journal_title：Biomacromolecules

authors： Zhao J,Bolisetty S,Isabettini S,Kohlbrecher J,Adamcik J,Fischer P,Mezzenga R

更新日期：2016-08-08 00:00:00

abstract：:PEO-PPO-PEO triblock copolymers have opposing effects on lipid membrane integrity: they can behave either as membrane sealants or as membrane permeabilizers. To gain insights into their biomembrane activities, the fundamental interactions between a series of PEO-based polymers and phospholipid vesicles were investigat...

journal_title：Biomacromolecules

authors： Wang JY,Marks J,Lee KY

更新日期：2012-09-10 00:00:00

abstract：:Water-soluble T-antigen containing glycopolymers [Gal beta(1,3)-GalNAc alpha) having a high degree of lipophilicity were synthesized from poly[N-(acryloxy)succinimide] (6-10) by amidation with an amine-ending T-antigen derivative (3) and various amines of increasing alkyl chain length (ammonia and methyl-, ethyl-, and...

journal_title：Biomacromolecules

authors： Baek MG,Roy R

更新日期：2000-01-01 00:00:00

abstract：:Bovine serum albumin (BSA) and poly(diallyldimethylammonium chloride) (PDADMAC) spontaneously form, over a range of ionic strength I and pH, dense fluids rich in both macroions. To study their nanostructure, these coacervates were prepared at low I and high pH (strong interaction) or at high I and lower pH (weaker int...

journal_title：Biomacromolecules

authors： Bohidar H,Dubin PL,Majhi PR,Tribet C,Jaeger W

更新日期：2005-05-01 00:00:00

abstract：:The time-dependent diffusion and mechanical properties of gelatin in solution, in the gel state, and during the sol/gel transition were determined using fluorescence recovery after photobleaching (FRAP) and rheology. The parameters in the experimental design were 2% w/w and 5% w/w gelatin concentration; 15, 20, and 25...

journal_title：Biomacromolecules

authors： Hagman J,Lorén N,Hermansson AM

更新日期：2010-12-13 00:00:00

abstract：:Surface pressure isotherms and structural and surface dilatational properties of three hydroxypropylmethycelluloses (HPMCs, called E4M, E50LV, and F4M) adsorbed films at the air-water interface were determined. In this work we present evidence that HPMC molecules are able to diffuse and saturate the air-water interfac...

journal_title：Biomacromolecules

authors： Pérez OE,Sánchez CC,Rodríguez Patino JM,Pilosof AM

更新日期：2006-01-01 00:00:00

abstract：:A way to convert the volume change of a biochemo-mechanical gel into the change in liquid column length was developed. Our trial sensor device consisted of a small compartment for incorporating the gel, a flow channel with a filled dye solution, and a poly(dimethylsiloxane) (PDMS) diaphragm by which the gel and the dy...

journal_title：Biomacromolecules

authors： Suzuki H,Kumagai A,Ogawa K,Kokufuta E

更新日期：2004-03-01 00:00:00

abstract：:Cationic derivatives of cellulose and other polysaccharides are attractive targets for biomedical applications due to their propensity for electrostatically binding with anionic biomolecules, such as nucleic acids and certain proteins. To date, however, relatively few practical synthetic methods have been described fo...

journal_title：Biomacromolecules

authors： Liu S,Liu J,Esker AR,Edgar KJ

更新日期：2016-02-08 00:00:00

abstract：:(1,3)(1,4)-β-D-Glucan (mixed-linkage glucan or MLG), a characteristic hemicellulose in primary cell walls of grasses, was investigated to determine both its role in cell walls and its interaction with cellulose and other cell wall polysaccharides in vitro. Binding isotherms showed that MLG adsorption onto microcrystal...

journal_title：Biomacromolecules

authors： Kiemle SN,Zhang X,Esker AR,Toriz G,Gatenholm P,Cosgrove DJ

更新日期：2014-05-12 00:00:00

abstract：:To investigate the influence of surface-functionalized substrates with nanostructures on the behaviors of mesenchymal stem cells, we conjugated bone morphogenetic protein 2 (BMP2) onto TiO(2) nanotubes with different diameter sizes of 30, 60, and 100 nm for in vitro study. Polydopamine was employed as the intermediate...

journal_title：Biomacromolecules

authors： Lai M,Cai K,Zhao L,Chen X,Hou Y,Yang Z

更新日期：2011-04-11 00:00:00

abstract：:This paper describes the synthesis and characterization of a novel organic polymer coating for the prevention of the growth of Pseudomonas aeruginosa on the solid surface of three-dimensional objects. Substrata were encapsulated with polyterpenol thin films prepared from terpinen-4-ol using radio frequency plasma enha...

journal_title：Biomacromolecules

authors： Bazaka K,Jacob MV,Truong VK,Wang F,Pushpamali WA,Wang JY,Ellis AV,Berndt CC,Crawford RJ,Ivanova EP

更新日期：2010-08-09 00:00:00