Abstract:
:To investigate the influence of surface-functionalized substrates with nanostructures on the behaviors of mesenchymal stem cells, we conjugated bone morphogenetic protein 2 (BMP2) onto TiO(2) nanotubes with different diameter sizes of 30, 60, and 100 nm for in vitro study. Polydopamine was employed as the intermediate layer for the conjugation of BMP2. The successful conjugation of BMP2 onto TiO(2) nanotubes was revealed by field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Immunofluorescence staining of vinculin, osteocalcin (OCN), and osteopontin (OPN) revealed that BMP2-functionalized TiO(2) nanotubes was favorable for cell growth. More importantly, MSCs cultured onto BMP2-functionalized TiO(2) nanotubes displayed significantly higher (p < 0.05 or p < 0.01) differentiation levels of ALP and mineralization after 7 and 14 day cultures, respectively. The results suggested that surface functionalization of TiO(2) nanotubes with BMP2 was beneficial for cell proliferation and differentiation. The approach presented here has potential application for the development of titanium-based implants for enhanced bone osseointegration.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Lai M,Cai K,Zhao L,Chen X,Hou Y,Yang Zdoi
10.1021/bm1014365subject
Has Abstractpub_date
2011-04-11 00:00:00pages
1097-105issue
4eissn
1525-7797issn
1526-4602journal_volume
12pub_type
杂志文章abstract::Cellulose-based dendrimerized material was prepared and its quality was assessed by determining the number of amine functional groups incorporated. Based on the results for a series of preparations, the material was obtained in a highly reproducible manner thanks to the particular chemical construction method used. Th...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701380a
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
pub_type: 杂志文章
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更新日期:2016-03-14 00:00:00
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
pub_type: 杂志文章
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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journal_title:Biomacromolecules
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