Abstract：
:Monoclonal antibodies (mAb) are a major focus of the pharmaceutical industry, and polyclonal immunoglobulin G (IgG) therapy is used to treat a wide variety of health conditions. As some individuals require mAb/IgG therapy their entire life, there is currently a great desire to formulate antibodies for bolus injection rather than infusion. However, to achieve the required doses, very concentrated antibody solutions may be required. Unfortunately, mAb/IgG self-assembly at high concentration can produce an unacceptably high viscosity for injection. To address this challenge, this study expands the concept of "dynamic covalent chemistry" to "dynamic bioconjugation" in order to reduce viscosity by interfering with antibody-antibody interactions. Ultra-sub-stoichiometric amounts of dynamic PEGylation agents (down to the nanomolar) significantly reduced the viscosity of concentrated antibody solutions by interfering with oligomerization.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Gong Y,Soleymani Abyaneh H,Drossis N,Niederquell A,Kuentz M,Leroux JC,de Haan HW,Gauthier MAdoi
10.1021/acs.biomac.9b00867subject
Has Abstractpub_date
2019-09-09 00:00:00pages
3557-3565issue
9eissn
1525-7797issn
1526-4602journal_volume
20pub_type
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