Abstract：
:Photoinitiated polymerization remains a robust method for fabrication of hydrogels, as these reactions allow facile spatial and temporal control of gelation and high compatibility for encapsulation of cells and biologics. The chain-growth reaction of macromolecular monomers, such as acrylated PEG and hyaluronan, is commonly used to form hydrogels, but there is growing interest in step-growth photopolymerizations, such as the thiol-ene "click" reaction, as an alternative. Thiol-ene reactions are not susceptible to oxygen inhibition and rapidly form hydrogels using low initiator concentrations. In this work, we characterize the differences in recovery of bioactive proteins when exposed to similar photoinitiation conditions during thiol-ene versus acrylate polymerizations. Following exposure to chain polymerization of acrylates, lysozyme bioactivity was approximately 50%; after step-growth thiol-ene reaction, lysozyme retained nearly 100% of its prereaction activity. Bioactive protein recovery was enhanced 1000-fold in the presence of a thiol-ene reaction, relative to recovery from solutions containing identical primary radical concentrations, but without the thiol-ene components. When the cytokine TGFβ was encapsulated in PEG hydrogels formed via the thiol-ene reaction, full protein bioactivity was preserved.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
McCall JD,Anseth KSdoi
10.1021/bm300671ssubject
Has Abstractpub_date
2012-08-13 00:00:00pages
2410-7issue
8eissn
1525-7797issn
1526-4602journal_volume
13pub_type
杂志文章abstract：:Although research on nanopaper structures from cellulose nanofibrils (CNFs) is well established, the mechanical behavior is not well understood, especially not when CNF is combined with hard nanoparticles. Cationic CNF (Q-CNF) was prepared and successfully decorated by anionic nanodiamond (ND) nanoparticles in hydroco...
journal_title：Biomacromolecules
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journal_title：Biomacromolecules
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journal_title：Biomacromolecules
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journal_title：Biomacromolecules
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journal_title：Biomacromolecules
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journal_title：Biomacromolecules
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更新日期：2010-11-08 00:00:00
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journal_title：Biomacromolecules
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更新日期：2011-08-08 00:00:00
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更新日期：2009-11-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
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更新日期：2008-10-01 00:00:00
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journal_title：Biomacromolecules
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