Abstract：
:Enzymatic adsorption and degradation of three types of aliphatic polyester films and two types of polyolefin films by the extracellular PHB depolymerase from Alcaligenes faecalis T1 have been studied by using a quartz crystal microbalance (QCM) technique. Hydrolysis of poly[(R)-3-hydroxybutyrate] was quantitatively followed by the QCM technique. Adsorption of the enzymes to films was also quantitatively detected by the QCM. Kinetic study on enzymatic adsorption suggests that the PHB depolymerase binds to the substrates not only by hydrophobic interaction but also by specific interaction between the ester bonds of polyesters and the binding domain of the enzyme. The results show that the QCM technique is a sensitive tool to study enzymatic degradation kinetics of biodegradable polyesters.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Yamashita K,Aoyagi Y,Abe H,Doi Ydoi
10.1021/bm0000844keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
25-8issue
1eissn
1525-7797issn
1526-4602pii
bm0000844journal_volume
2pub_type
杂志文章abstract：:Electrospinning of cellulose nanocrystals (CNC)/poly(lactic acid) (PLA) emulsions has been demonstrated to be an effective dispersion and alignment method to control assembly of CNC into continuous composite ultrafine fibers. CNC-PLA nanocomposite random-fiber mats and aligned-fiber yarns were prepared by emulsion ele...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm400540v
更新日期：2013-11-11 00:00:00
abstract：:Cellular model systems are essential platforms used across multiple research fields for exploring the fundaments of biology and biochemistry. Here, we present giant plasma membrane vesicles (GPMVs) as a platform of cell-like compartments that will facilitate the study of particles within a biorelevant environment and ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00624
更新日期：2021-01-11 00:00:00
abstract：:Surface-immobilized liposome layers are of interest for various potential applications such as localized drug delivery, but their characterization is challenging. We have employed an AFM method and fluorescent dye release to analyze anchored liposomes. In addition, we studied whether the liposomes are surface-bound so...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049941k
更新日期：2004-07-01 00:00:00
abstract：:Type I collagen is explored heavily for use in biomaterials, but the role of other extracellular matrix components in regulating collagen organization is gaining attention. We show that as the ratio of type III to type I collagen increases, fibril diameter decreases. A mixture of the two collagen types results in a mo...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm800888u
更新日期：2009-01-12 00:00:00
abstract：:Biopolymers bearing hydrophobic side-chains, such as hydrophobically modified chitosan (hmC), can connect liposomes into a gel network via hydrophobic interactions. In this paper, we show that such liposome gels possess an attractive combination of properties for certain drug delivery applications. Their shear-thinnin...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm301143d
更新日期：2012-10-08 00:00:00
abstract：:Cationic kraft lignin (CKL) macromolecules were produced via polymerizing kraft lignin (KL) with [2-(acryloyloxy)ethyl]trimethylammonium chloride (ATAC) or [2-(methacryloyloxy)ethyl]trimethylammonium methyl sulfate (METAM). Despite slightly different charge densities (2.3-2.5 mmol/g) of CKL, lignin-METAM (KL-METAM) ha...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01016
更新日期：2019-10-14 00:00:00
abstract：:A series of copolymers of trimethylene carbonate (TMC) and L-lactide (LLA) were synthesized and evaluated as scaffolds for the production of artificial blood vessels. The polymers were end-functionalized with acrylate, cast into films, and cross-linked using UV light. The mechanical, degradation, and biocompatibility ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201291e
更新日期：2011-11-14 00:00:00
abstract：:A lipid bilayer was deposited on a 3 μm diameter polystyrene (PS) bead via hydrophobic anchoring of bicelles containing oxyamine-bearing cholesteric moieties reacting with the aldehyde functionalized bead surface. Discoidal bicelles were formed by mixing dimyristoylphosphatidylcholine (DMPC), dihexanoylphosphatidylcho...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00042
更新日期：2015-03-09 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700690p
更新日期：2007-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00327
更新日期：2018-04-09 00:00:00
abstract：:It is demonstrated that the molecular solubility of softwood hemicelluloses is significantly influenced by pretreatment of the fibers, extraction, and downstream processing. To quantify these effects, four hemicellulose samples were extracted from different thermomechanical pulps of Norway spruce. The molecular solubi...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b00088
更新日期：2018-04-09 00:00:00
abstract：:The Bacillus subtilis protease Proleather FG-F catalyzed the transesterification of inulin with vinyl acrylate (VA) in dimethylformamide (DMF). The reaction conversion for different VA concentrations was greater than 57% after 96 h at 50 degrees C. The degree of substitution (DS, defined as the amount of acrylate grou...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm010150h
更新日期：2002-03-01 00:00:00
abstract：:Plastics ending up in soil or landfill environment will eventually be degraded. At the same time a slow migration of additives from the plastics will occur. The additives and the degradation products of these and the polymer are potential compounds for bioconversion. To predict the rate of the migration of antioxidant...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0000283
更新日期：2000-10-01 00:00:00
abstract：:We investigate lysozyme-lysozyme and lysozyme-salt interactions in electrolyte solutions using a molecular-thermodynamic model. An equation of state based on the statistical mechanical perturbation theory is applied to describe the interactions. The perturbation term includes a new square-well potential of mean force,...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm0300406
更新日期：2003-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00589
更新日期：2019-07-08 00:00:00
abstract：:Coiled coils are a common structural motif in many natural proteins that can also be utilized in the design and preparation of drug delivery systems for the noncovalent connection of two macromolecules. In this work, two different pairs of peptides forming coiled coil hetero-oligomers were designed, synthesized, and c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500436p
更新日期：2014-07-14 00:00:00
abstract：:The microstructure and the rheological properties of pure HM (high methoxyl) and LM (low methoxyl) pectin gels and of mixed HM/LM pectin gels have been investigated. Gel formation of either the HM or LM pectin, or both, was initiated in the mixed gels by varying the sucrose and Ca(2+) content. The microstructure was c...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm020044v
更新日期：2002-11-01 00:00:00
abstract：:Immunological detection has been developed into a sensitive and versatile technique and is based on two requisite elements: targeting antibodies and an indicator, usually in the form of horseradish peroxidase or alkaline phosphatase. The specificity and turnover rate of these enzymes provide an efficient means of sign...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401511a
更新日期：2014-01-13 00:00:00
abstract：:Controlled reeling is a powerful tool to investigate the details of silk processing. However, consistent forced reeling of silkworms is hindered by the significant degree of behaviorally induced variation caused by the animal. This paper proposes silkworm paralysis as a novel method to control the animal and thus in v...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm401013k
更新日期：2013-10-14 00:00:00
abstract：:Hydrogels are promising materials for biomedical applications such as tissue engineering and controlled drug release. In the past two decades, the peptide hydrogel subclass has attracted an increasing level of interest from the scientific community because of its numerous advantages, such as biocompatibility, biodegra...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b01840
更新日期：2017-03-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201712z
更新日期：2012-03-12 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700967k
更新日期：2008-01-01 00:00:00
abstract：:Understanding the self-assembly mechanism of caged proteins provides clues to develop their potential applications in nanotechnology, such as a nanoscale drug delivery system. The E2 protein from Bacillus stearothermophilus , with a virus-like caged structure, has drawn much attention for its potential application as...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201587q
更新日期：2012-03-12 00:00:00
abstract：:Recent research indicates that the progression of Parkinson's disease can start from neurons of the enteric nervous system, which are in close contact with the gastrointestinal epithelium: α-synuclein molecules can be transferred from these epithelial cells in a prion-like fashion to enteric neurons. Thin mucus layers...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00905
更新日期：2019-12-09 00:00:00
abstract：:We examine the protein distribution within an electrospun polymer nanofiber using polyvinyl alcohol and bovine serum albumin as a model system. We hypothesize that the location of the protein within the nanofiber can be controlled by carefully selecting the pH and the applied polarity of the electric field as the pH a...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2017146
更新日期：2012-05-14 00:00:00
abstract：:The inferior in vivo stability of micellar drugs has been a prime challenge for their application in targeted drug delivery. Here we report on novel galactose-decorated covalently cross-linked biodegradable micelles based on photo-cross-linkable poly(ethylene glycol)-b-poly(acryloyl carbonate)-b-poly(ε-caprolactone) (...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm2006856
更新日期：2011-08-08 00:00:00
abstract：:Genome-editing technologies hold tremendous potential for treating genetic diseases. However, the efficient and safe delivery of genome-editing elements to the location of interest, and the achievement of specific targeted gene correction without off-target side effect remains a big challenge. In this Perspective, we ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b00783
更新日期：2019-09-09 00:00:00
abstract：:Thermoresponsive polymers with lower critical solution temperatures (LCSTs) are of significant interest for a wide range of applications from sensors to drug delivery vehicles. However, the most widely investigated LCST polymers have nondegradable backbones, limiting their applications in vivo or in the environment. D...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c00899
更新日期：2020-09-14 00:00:00
abstract：:It was aim of the study to investigate the in vivo potential of a novel insulin-thiomer complex nanoparticulate delivery system. Insulin loaded nanoparticles were obtained by the formation of hydrogen bonds between poly(vinyl pyrrolidone) (PVP) and poly(acrylic acid)-cysteine (PAA-Cys) or poly(acrylic acid) (PAA), res...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm700916h
更新日期：2008-01-01 00:00:00
abstract：:Selectively spooling single plasmid DNA (pDNA), as a giant polyelectrolyte, into a nanosized toroidal structure or folding it into a rod-like structure has been accomplished by polyion complexation with block catiomers to form polymeric micelles in varying NaCl concentrations. The interactive potency between the pDNA ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b00491
更新日期：2015-09-14 00:00:00
